Effect of captopril on oxidative damage in pentylenetetrazole induced SHSY-5Y human neuroblastoma cell line

Abstract

Epilepsy is a crucial brain disorder that seizures could cause a neuronal loss in the hippocampus. Oxidative stress has an important role in the pathology of this way. The aim of this study was to investigate the neuroprotective effect of captopril, on pentylenetetrazole (PTZ) induced epileptic seizures in SH-SY5Y cell line. Method: In this XTT cell viability assay, captopril was performed in vitro SH-SY5Y cell culture to evaluate PTZ-induced neurotoxicity. Tissue TOS concentrations at the cell supernatants were quantified with the automated assay method. Hydrogen peroxide was used for the calibration of the assay. Results: When XTT cell viability results were evaluated, captopril did not affect neuronal viability in SH-SY5Y cell line. Moreover, captopril did not have significant effect on TOS levels (***P>0. 001) Conclusions: Results showed that, captopril did not have neuroprotective properties in SH-SY5Y cell line after PTZ-induced neurotoxicity.

Assessment of mechanism involved in the apoptotic and anti-cancer activity of Quercetin and Quercetin-loaded chitosan nanoparticles

“…Total antioxidant status (TAS) and Total oxidant status (TOS) assessment in Quercetin and Quercetinloaded NP treated and untreated SH-SY5Y cells The total antioxidant status assay kit (Rel Assay Diagnostics, Turkey) and total oxidant status assay kit (Rel Assay Diagnostics, Turkey) were used to evaluate TAS and TOS quantities in Quercetin and Quercetin-loaded NP treated and untreated SH-SY5Y cells, respectively [33]. SH-SY5Y cells were treated with 2 μg/mL Quercetin and Quercetin-loaded NP for 24 h. For TAS and TOS, the data were expressed as mmol Trolox Equiv./L and mol H 2 O 2 Equiv./L, respectively.…”