Effect of calcium-coacervate infiltration of artificial enamel caries lesions in de- and remineralizing conditions

Kharbot, Basel; Bulgun, Neziha; Cölfen, Helmut; Paris, Sebastian

doi:10.1016/j.jdent.2024.104838

Cited by 2 publications

(7 citation statements)

References 52 publications

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“…Previously, we were able to validate the X-ray diffraction patterns of HAP in CCinfiltrated artificial enamel lesions (Prause et al, in review) [26]. While the limited amount of Ca 2+ ions that can be transferred into the lesions is most probably the reason we only observed moderate immediate effects, we were able to show the protective effects of CCs against further demineralization with highly mineralized bands within the lesion bodies.…”

Section: Discussionsupporting

confidence: 59%

“…An a priori power analysis was conducted using Sample Size Calculator (Version 1.061) for sample size estimation. Based on a previous study [26], the effect size was considered to be small using Cohen's (1988) criteria. With a significance level of α = 0.05 and power = 0.80, the minimum sample size required was calculated to be 15 assuming a drop-off rate of 10%.…”

Section: Methodsmentioning

confidence: 99%

“…Specimens were subsequently cut perpendicularly to the lesions, with one half serving as baseline control (BL) while the other half (effect, E) underwent pHC. To test the reproducibility of a previous study in one group (CC + DS), the E-halves of CC-treated lesions were exposed to a demineralizing solution instead of pHC [26]. The change in integrated mineral loss by different treatment modalities was analyzed using transversal microradiography (TMR).…”

Section: Methodsmentioning

confidence: 99%

“…In this study, complex coacervation occurs based on the electrostatic interaction of polyacrylic acid and calcium ions in an aqueous solution, forming PAA-Ca 2+ droplets [40][41][42][43][44]. The infiltration of enamel caries lesions with a calcium coacervate emulsion (CC) in our previous studies did not yield significant, immediate remineralization, but rather showed protective effects against further demineralization with characteristic mineralized bands within the lesion bodies [26]. However, the question of whether this effect was achieved by the CC or one of its components could not be definitively answered.…”

Section: Introductionmentioning

confidence: 97%

“…Clinically, fluorides remain the cornerstone for early caries management with the highest level of supporting evidence, although they seem to be the most effective when used in combination with other treatment measures, such as resin infiltration, especially in high-risk patients [21][22][23][24][25]. In previous studies, we attempted to initiate remineralization processes in enamel lesions exploring bio-inspired possibilities of non-classical crystallization (NCC) pathways through liquid precursors [26].…”

Section: Introductionmentioning

confidence: 99%

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Biomimetic Remineralization of Artificial Caries Lesions with a Calcium Coacervate, Its Components and Self-Assembling Peptide P11-4 In Vitro

Kharbot,

Askar,

Gruber

et al. 2024

Bioengineering

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The application of calcium coacervates (CCs) may hold promise for dental hard tissue remineralization. The aim of this study was to evaluate the effect of the infiltration of artificial enamel lesions with a CC and its single components including polyacrylic acid (PAA) compared to that of the self-assembling peptide P11-4 in a pH-cycling (pHC) model. Enamel specimens were prepared from bovine incisors, partly varnished, and stored in demineralizing solution (DS; pH 4.95; 17 d) to create two enamel lesions per sample. The specimens were randomly allocated to six groups (n = 15). While one lesion per specimen served as the no-treatment control (NTC), another lesion (treatment, T) was etched (H3PO4, 5 s), air-dried and subsequently infiltrated for 10 min with either a CC (10 mg/mL PAA, 50 mM CaCl2 (Ca) and 1 M K2HPO4 (PO4)) (groups CC and CC + DS) or its components PAA, Ca or PO4. As a commercial control, the self-assembling peptide P11-4 (CurodontTM Repair, Credentis, Switzerland) was tested. The specimens were cut perpendicularly to the lesions, with half serving as the baseline (BL) while the other half was exposed to either a demineralization solution for 20 d (pH 4.95; group CC + DS) or pHC for 28 d (pH 4.95, 3 h; pH 7, 21 h; all five of the other groups). The difference in integrated mineral loss between the lesions at BL and after the DS or pHC, respectively, was analyzed using transversal microradiography (ΔΔZ = ΔZpHC − ΔZbaseline). Compared to the NTC, the mineral gain in the T group was significantly higher in the CC + DS, CC and PAA (p < 0.05, Wilcoxon). In all of the other groups, no significant differences between treated and untreated lesions were detected (p > 0.05). Infiltration with the CC and PAA resulted in a consistent mineral gain throughout the lesion body. The CC as well as its component PAA alone promoted the remineralization of artificial caries lesions in the tested pHC model. Infiltration with PAA further resulted in mineral gain in deeper areas of the lesion body.

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Section: Discussionsupporting

confidence: 59%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Biomimetic Remineralization of Artificial Caries Lesions with a Calcium Coacervate, Its Components and Self-Assembling Peptide P11-4 In Vitro

Kharbot,

Askar,

Gruber

et al. 2024

Bioengineering

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Antimicrobial, remineralization, and infiltration: advanced strategies for interrupting dental caries

Yang,

Li,

et al. 2024

Medical Review

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Dental caries, driven by plaque biofilm, poses a major oral health challenge due to imbalance in mineralization and demineralization. The primary objective in caries management is to maintain biofilm homeostasis while facilitating the repair and regeneration of dental hard tissues, thus restoring both structural integrity and functionality of affected teeth. Though antimicrobial and remineralization approaches haven shown promise, their standalone utilization without concurrent bacterial control or rebalancing lacks an integrated strategy to effectively arrest caries progression. Furthermore, according to the principles of minimally invasive dentistry, treatment materials should exhibit high permeability to ensure optimal sealing of demineralized tooth surfaces. The concept of interrupting dental caries (IDC) has emerged as a holistic approach, drawing upon extensive research encompassing three pivotal techniques: antibacterial strategies, remineralization therapies, and infiltration mechanisms, all of which are indispensable components in combating the progression of dental caries. In this review, we provide a comprehensive overview of the mechanisms and applications of antibacterial, remineralization, and infiltration technologies within the context of caries management. Additionally, we summarize advanced materials that align with the IDC concept, aiming to offer valuable insights for designing next-generation materials adept at preventing or halting caries progression efficiently.

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Effect of calcium-coacervate infiltration of artificial enamel caries lesions in de- and remineralizing conditions

Cited by 2 publications

References 52 publications

Biomimetic Remineralization of Artificial Caries Lesions with a Calcium Coacervate, Its Components and Self-Assembling Peptide P11-4 In Vitro

Biomimetic Remineralization of Artificial Caries Lesions with a Calcium Coacervate, Its Components and Self-Assembling Peptide P11-4 In Vitro

Antimicrobial, remineralization, and infiltration: advanced strategies for interrupting dental caries

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