Effect of calcium carbonate particle shape on phagocytosis and pro-inflammatory response in differentiated THP-1 macrophages

Tabei, Yosuke; Sugino, Sakiko; Eguchi, Kenichiro; Tajika, Masahiko; Abe, Hiroko; Nakajima, Yoshihiro; Horie, Masanori

doi:10.1016/j.bbrc.2017.06.069

Cited by 19 publications

(11 citation statements)

References 29 publications

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“…Inflammation in turn triggers microcalcification [ 2 , 3 ]; thus, the AGE/RAGE cascade mediates vascular calcification oxidative stress mechanisms [ 2 , 4 , 5 ]. Endogenous mineral particles can activate macrophages [ 6 , 7 ] and neutrophils [ 8 , 9 ], inducing ROS and cytokine production. Biomineralization is characterized by the inclusion of proteins and other biomolecules into the calcifications [ 10 ], modulating their neutrophil-activating capacity [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin

Mikhalchik

Иванов

Borodina

et al. 2022

IJMS

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Hyperglycemia-induced protein glycation and formation of advanced glycation end-products (AGEs) plays an important role in the pathogenesis of diabetic complications and pathological biomineralization. Receptors for AGEs (RAGEs) mediate the generation of reactive oxygen species (ROS) via activation of NADPH-oxidase. It is conceivable that binding of glycated proteins with biomineral particles composed mainly of calcium carbonate and/or phosphate enhances their neutrophil-activating capacity and hence their proinflammatory properties. Our research managed to confirm this hypothesis. Human serum albumin (HSA) was glycated with methylglyoxal (MG), and HSA-MG was adsorbed onto mineral microparticles composed of calcium carbonate nanocrystals (vaterite polymorph, CC) or hydroxyapatite nanowires (CP). As scopoletin fluorescence has shown, H2O2 generation by neutrophils stimulated with HSA-MG was inhibited with diphenyleneiodonium chloride, wortmannin, genistein and EDTA, indicating a key role for NADPH-oxidase, protein tyrosine kinase, phosphatidylinositol 3-kinase and divalent ions (presumably Ca2+) in HSA-MG-induced neutrophil respiratory burst. Superoxide anion generation assessed by lucigenin-enhanced chemiluminescence (Luc-CL) was significantly enhanced by free HSA-MG and by both CC-HSA-MG and CP-HSA-MG microparticles. Comparing the concentrations of CC-bound and free HSA-MG, one could see that adsorption enhanced the neutrophil-activating capacity of HSA-MG.

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Section: Introductionmentioning

confidence: 99%

Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin

Mikhalchik

Иванов

Borodina

et al. 2022

IJMS

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“…On the contrary, several studies have indicated that IL-8 gene expression is induced by the increase of [Ca 2+ ] i in various cell types, including neutrophils, monocytes, and epithelial cells 50–53 . We also reported that [Ca 2+ ] i elevation resulted in an increase of IL-8 production in macrophages using calcium-based fine particles 54 . Then, to investigate whether [Ca 2+ ] i elevation by High Fe-CNT is caused by IL-8 stimulus, dHL-60 cells were pre-treated with BAY 11–7082, an inhibitor of NF-κB activation, for 30 min and subsequently exposed to High Fe-CNT for an additional 24 h. Treatment of dHL-60 cells with BAY 11–7082 resulted in the attenuation of the increase of IL-8 production caused by High Fe-CNT exposure (Fig.…”

Section: Resultsmentioning

confidence: 82%

Effect of iron overload from multi walled carbon nanotubes on neutrophil-like differentiated HL-60 cells

Tabei

Fukui

Nishioka

et al. 2019

Sci Rep

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Multi walled carbon nanotubes (MWCNTs) are one of the most intensively explored nanomaterials because of their unique physical and chemical properties. Due to the widespread use of MWCNTs, it is important to investigate their effects on human health. The precise mechanism of MWCNT toxicity has not been fully elucidated. The present study was designed to examine the mechanisms of MWCNT toxicity toward human promyelocytic leukemia HL-60 cells. First, we found that MWCNTs decreased the viability of neutrophil-like differentiated HL-60 cells but not undifferentiated HL-60 cells. Because neutrophil-like differentiated HL-60 cells exhibit enhanced phagocytic activity, the cytotoxicity of MWCNTs is dependent on the intracellularly localized MWCNTs. Next, we revealed that the cytotoxicity of MWCNTs is correlated with the intracellular accumulation of iron that is released from the engulfed MWCNTs in an acidic lysosomal environment. The intracellular accumulation of iron was repressed by treatment with cytochalasin D, a phagocytosis inhibitor. In addition, our results indicated that iron overload enhanced the release of interleukin-8 (IL-8), a chemokine that activates neutrophils, and subsequently elevated intracellular calcium concentration ([Ca2+]i). Finally, we found that the sustained [Ca2+]i elevation resulted in the loss of mitochondrial membrane potential and the increase of caspase-3 activity, thereby inducing apoptotic cell death. These findings suggest that the iron overload caused by engulfed MWCNTs results in the increase of IL-8 production and the elevation of [Ca2+]i, thereby activating the mitochondria-mediated apoptotic pathway.

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“…According to the previous report, ZnO NP has a diameter of 20-50 nm and a specific surface area of 31.5 m 2 /g, and ZnO-Si NP has a diameter of 10-100 nm and a specific surface area of 23.2 m 2 /g [35]. The NP-medium dispersions were prepared as described previously [36]. In brief, ZnO NPs or ZnO-Si NPs were dispersed in 1% bovine serum albumin at the concentration of 10 mg/mL by sonication prior to use.…”

Section: Preparation Of Zno or Zno-si Dispersionsmentioning

confidence: 99%

Development of fibrin hydrogel–based in vitro bioassay system for assessment of skin permeability to and pro-inflammatory activity mediated by zinc ion released from nanoparticles

et al. 2020

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Nanoparticles (NPs) are promising products in industry and medicine due to their unique physicochemical properties. In particular, zinc oxide (ZnO) NPs are extensively incorporated into sunscreens to protect the skin from exposure to ultraviolet radiation. However, there are several health concerns about skin penetration and the resultant toxicity. As methodologies for evaluating NP toxicity are under development, it is difficult to fully assess the toxicity of ZnO NPs toward humans. In this study, we developed a platform to simultaneously detect skin permeability to and pro-inflammatory activity mediated by zinc ion released from NPs. First, we generated a stable reporter cell line expressing green fluorescent protein (GFP) under the control of interleukin-8 (IL-8) promoter activity. The expression levels of GFP induced by zinc reflected the endogenous IL-8 expression levels and the pro-inflammatory responses. Next, we found that fibrin hydrogel can reproduce permeability to zinc ion of a human skin equivalent model and is therefore a promising material to assess skin permeability to zinc ion. Then, we constructed a fibrin hydrogel-based in vitro bioassay system for the simultaneous detection of skin permeability to and pro-inflammatory activity mediated by zinc ion released from NPs by using a stable reporter cell line and a fibrin hydrogel layer. This bioassay system is a promising in vitro permeation test due to its technical simplicity and good predictability. Overall, we believe that our bioassay system can be widely used in the cosmetics and pharmaceutical industries.

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Effect of calcium carbonate particle shape on phagocytosis and pro-inflammatory response in differentiated THP-1 macrophages

Cited by 19 publications

References 29 publications

Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin

Neutrophil Activation by Mineral Microparticles Coated with Methylglyoxal-Glycated Albumin

Effect of iron overload from multi walled carbon nanotubes on neutrophil-like differentiated HL-60 cells

Development of fibrin hydrogel–based in vitro bioassay system for assessment of skin permeability to and pro-inflammatory activity mediated by zinc ion released from nanoparticles

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