Effect of calcium alginate concentration on viability and proliferation of encapsulated fibroblasts

Bohari, Siti Pauliena Mohd; Hukins, D.W.L.; Grover, Liam M.

doi:10.3233/bme-2011-0665

Cited by 51 publications

(46 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This observation is in line with previous reports of limited proliferation of cells encapsulated in alginate hydrogels [27,28]. Live/dead staining confirmed a high ratio of viable to dead cells 4 hours after seeding as well as after 2 weeks of culture in both scaffold types (Fig.…”

Section: Comparison Of Cell Spreading and Proliferation Within Silk Asupporting

confidence: 92%

Silk assembly integrates cells into a 3D fibrillar network that promotes cell spreading and proliferation

Johansson

Widhe

et al. 2018

Preprint

View full text Add to dashboard Cite

Tissues are built of cells integrated in an extracellular matrix (ECM) which provides a threedimensional (3D) fibrillar network with specific sites for cell anchorage. By genetic engineering, motifs from the ECM can be functionally fused to recombinant silk proteins. Such a silk protein, FN-silk, which harbours a motif from fibronectin, has the ability to selfassemble into fibrillar networks under physiological-like conditions. Herein we describe a method by which mammalian cells are added to the silk solution before assembly, and thereby get uniformly integrated between the formed fibrils. In the resulting 3D scaffold, the cells proliferate and spread out with tissue-like morphology. Elongated cells containing filamentous actin and defined focal adhesion points confirm proper cell attachment to the FN-silk. The cells remain viable in culture for at least 90 days. The method is also scalable to macro-sized 3D cultures. Silk fibers with integrated cells are both strong and extendable, with mechanical properties similar to that of artery walls. The described method enables both differentiation of stem-or precursor cells in 3D and facile co-culture of several different cell types. We show that inclusion of endothelial cells leads to the formation of vessel-like structures throughout the tissue constructs. Hence, silk-assembly in presence of cells constitutes a viable option for 3D culture of cells integrated in a fibrillary ECM-like network, with potential as base for engineering of functional tissue.

show abstract

Section: Comparison Of Cell Spreading and Proliferation Within Silk Asupporting

confidence: 92%

Silk assembly integrates cells into a 3D fibrillar network that promotes cell spreading and proliferation

Johansson

Widhe

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Alginate is one of the hydrogels used for the biofabrication of scaffolds used for tissue engineering applications, as reported in numerous studies [16][17][18][19][20][21][22][23]. Specifically, one of the approaches to improve the printability of alginate scaffolds fabricated by extrusion-based bioprinting method is to mix alginate with other types of hydrogels [24].…”

Section: Introductionmentioning

confidence: 99%

Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

et al. 2019

View full text Add to dashboard Cite

Extrusion-based bioprinting of hydrogel scaffolds is challenging due to printing-related issues, such as the lack of capability to precisely print or deposit hydrogels onto three-dimensional (3D) scaffolds as designed. Printability is an index to measure the difference between the designed and fabricated scaffold in the printing process, which, however, is still under-explored. While studies have been reported on printing hydrogel scaffolds from one or more hydrogels, there is limited knowledge on the printability of hydrogels and their printing processes. This paper presented our study on the printability of 3D printed hydrogel scaffolds, with a focus on identifying the influence of hydrogel composition and printing parameters/conditions on printability. Using the hydrogels synthesized from pure alginate or alginate with gelatin and methyl-cellulose, we examined their flow behavior and mechanical properties, as well as their influence on printability. To characterize the printability, we examined the pore size, strand diameter, and other dimensions of the printed scaffolds. We then evaluated the printability in terms of pore/strand/angular/printability and irregularity. Our results revealed that the printability could be affected by a number of factors and among them, the most important were those related to the hydrogel composition and printing parameters. This study also presented a framework to evaluate alginate hydrogel printability in a systematic manner, which can be adopted and used in the studies of other hydrogels for bioprinting.

show abstract

“…Alginate is well known for its ability to increase the shelflife of meat and meat products and has a wide range of application in food and pharmaceutical industries (Dalmoro, Barba, Lamberti, Grassi, & d'Amore, 2012;Xiao, Tong, & Lim, 2012). It is better known for its beneficial properties such as low toxicity, ability to gel under gentle conditions, controlling moisture transfer, good mechanical characteristics, emulsion stabilizers, and decrease oxygen transfer (Bohari, Hukins, & Grover, 2010;Galus & Lenart, 2013;Hambleton, Voilley, & Debeaufort, 2011). Chitosan is a linear polymer of mucopolysaccharide from animal origin composed of β-1-4 D-glucosamine units.…”

Section: Introductionmentioning

confidence: 99%

Development of enrobed fish products: Improvement of functionality of coated materials by added aquatic polymers

Hauzoukim

Xavier

Kannuchamy

et al. 2019

J Food Process Engineering

View full text Add to dashboard Cite

The study was aimed at evaluating three polymer hydrocolloids from aquatic sources in batter composition for developing enrobed fish fingers with less oil uptake and improved coating properties. Alginate, chitosan, and fish gelatin were incorporated at 1% (wt/wt) level in batter mix and coating parameters such as coating pickup, adhesion degree, frying loss, and cooked yield were studied. Proximate composition, chemical quality parameters, and oil absorption/oil reduction due to hydrocolloids were studied, and the results indicated that chitosan‐treated fish fingers are found to have better coating functions, biochemical quality with lower total volatile basic nitrogen value (6.02 mg/100 g), peroxide value (0.05 meq O2/kg), and thiobarbituric acid reactive substances (0.15 MDA/kg fat) than alginate and gelatin treated samples. The results indicated that lowest fat uptake (3.73%), lowest hardness value, and highest fat reduction were observed in fish finger coated with chitosan containing batter. Among the three aquatic polymers evaluated, chitosan was found to be more effective in oil reduction and physicochemical quality improvement as compared to alginate and fish gelatin. Practical applications This study highlights the screening and evaluation of three biological macromolecules of aquatic origins such as alginate, chitosan, and gelatin for the development of battered and breaded fish finger with better functional qualities including minimum oil absorption and improved shelf‐life. The outcome of this work can be used to extend the development of functional fishery products, and it can be utilized to extend the market potential of alginate and chitosan and promote the battered and breaded product consumption with more health benefits.

show abstract

Effect of calcium alginate concentration on viability and proliferation of encapsulated fibroblasts

Cited by 51 publications

References 28 publications

Silk assembly integrates cells into a 3D fibrillar network that promotes cell spreading and proliferation

Silk assembly integrates cells into a 3D fibrillar network that promotes cell spreading and proliferation

Printability of 3D Printed Hydrogel Scaffolds: Influence of Hydrogel Composition and Printing Parameters

Development of enrobed fish products: Improvement of functionality of coated materials by added aquatic polymers

Contact Info

Product

Resources

About