Effect of caffeine on the post-thaw motility of buffalo spermatozoa

Fattouh, El-S.M.; Abdou, M.S.S.

doi:10.1016/0093-691x(91)90444-i

Cited by 11 publications

(3 citation statements)

References 10 publications

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“…Also, Daader et al (1982) found that the highest sperm viability with the highest level of catalse addition, being 38, 42, 50, 52 and 58% with levels of 0, 125, 250, 500 and 1000 U/ml, respectively. Moreover, El-Gaafary et al (1990) and Fatouh and Abdou (1991) observed an increase in sperm motility of diluted bull semen supplemented with different levels of catalase to egg yolk extender in semen stored at 5°C.…”

Section: Effect Of Types and Levels Of Antioxidants On: Sperm Motilitmentioning

confidence: 93%

Effect of Different Types and Levels of Antioxidants on Viability and Acrosomal Status of Frozen-Thawed Spermatozoa of Buffalo Bulls.

Abdel-Khalek,¹,

Eid²,

Gabr³

et al. 2009

Journal of Animal and Poultry Production

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Semen was collected twice daily (100 ejaculates) from 5 sexually mature buffalo bulls (7-10 y old and 550-620 kg LBW) during the interval from January to April 2007 to study the effect of different types and levels of antioxidants on sperm motility and acrosome status in post-thawed buffalo semen. The main extender used for semen dilution was Tris-egg-yolk-citrate containing 7% glycerol. Total of 10 extenders, 9 with 3 types and 3 levels of antioxidants, including catalase (250, 500 and 1000 IU), glutathione (GSH, 0.4, 0.8 and 1.2 mM) and ascorbic acid (AA, 0.5, 1.0 and 1.5 g/l) were compared to unsupplemented extender (control). Semen was extended with different extenders, frozen in Liquid nitrogen (-196 o C) and thawed at rates of 15/60, 35/30 and 55/15 o C/sec. Percentages of sperm motility and damage acrosome were determined in post-thawed semen. Conception rate was detected in 100 sexually mature buffalo cows. Results revealed that adding all antioxidants to semen extenders increased percentage of sperm motility compared with the control, except for the highest level of AA (1.5 g/l), which did not affect sperm motility percentage. The best improvement was catalase at a level of 1000 IU (62.1%). Adding all levels of catalase and the lowest level of GSH (0.4 mM) or AA (0.5 g/l) decreased percentage of sperm with damage acrosome as compared to the control. The highest level of catalase (1000 IU) showed the highest impact on reducing percentage of sperm with damage acrosome (6.0%). Sperm motility percentage was higher with a thawed rate of 55/15 than 35/30 than 15 o C/60 sec, being 43.0, 32.11 and 27.0%, respectively). Adding catalase (1000 IU) or AA (0.5 or 1.0 g/l) increased sperm motility percentage in semen thawed by different rates, being 38.3, 37.4 and 37.1%, respectively. The highest conception rate (80%) was obtained from buffalo cows inseminated with semen extended using catalase (1000 IU). In conclusion, Tris-based extender containing catalase at a level of 1000 IU in frozen semen thawed at a rate of 55 o C/15 sec showed the highest post-thawing motility and the best fertilizing capacity of buffalo spermatozoa.

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Section: Effect Of Types and Levels Of Antioxidants On: Sperm Motilitmentioning

confidence: 93%

Effect of Different Types and Levels of Antioxidants on Viability and Acrosomal Status of Frozen-Thawed Spermatozoa of Buffalo Bulls.

Abdel-Khalek,¹,

Eid²,

Gabr³

et al. 2009

Journal of Animal and Poultry Production

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“…Indeed, in vitro tests on animal models have reported that the addition of caffeine to semen samples increases motility and stimulates capacitation and spontaneous acrosome reaction of the spermatozoa (Yamaguchi et al ., ; Yamaguchi & Funahashi, ). In addition, Fattouh & Abdou () demonstrated that the addition of caffeine to buffalo semen post‐cryopreservation can increase sperm motility by up to 114% compared to control samples.…”

Section: Introductionmentioning

confidence: 99%

Effects of caffeine supplementation in post-thaw human semen over different incubation periods

Pariz

Hallak

2016

Andrologia

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This study aimed to evaluate the effects of caffeine supplementation in post-cryopreservation human semen over different incubation periods. After collection by masturbation, 17 semen samples were analysed according to World Health Organization criteria, processed and cryopreserved with TEST-yolk buffer (1 : 1) in liquid nitrogen. After a thawing protocol, samples were incubated with 2 mm of caffeine for 0, 5, 15, 30 or 60 min, followed by analysis of motility and mitochondrial activity using 3,3'-diaminobenzidine (DAB). Mean variance analysis was performed, and P < 0.05 was the adopted significance threshold. Samples incubated for 15 min showed increased progressive motility compared to other periods of incubation, as well as a reduced percentage of immotile spermatozoa (P < 0.05). In samples incubated for 5 min, increased mitochondrial activity above 50% was observed (DABI and DABII). Although cryosurvival rates were low after the cryopreservation process, incubation with caffeine was associated with an increase in sperm motility, particularly 15-min incubation, suggesting that incubation with caffeine can be an important tool in patients with worsening seminal quality undergoing infertility treatment.

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“…Caffeine, a metabolic stimulant enhances motility, respiration and fructolysis of epididymal and ejaculated spermatozoa of cattle and buffalo bulls [11]- [13]. It has also been reported to improve progressive motility of both fresh and preserved sperms of various species [14] [15].…”

Section: Introductionmentioning

confidence: 99%

Incorporation of Ascorbic Acid, Caffeine and Chloroquine Diphosphate in Dilutor Improves Structural and Functional Status of Frozen Semen

Srivastava¹,

Kumar²

2014

OALib

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Effect of supplementation of ascorbic acid, caffeine and chloroquine diphosphate in the dilutor on post-thaw quality of spermatozoa was studied. Thirty-two semen ejaculates (8 each from 2 Holstein Friesian and 2 crossbred; 50% Friesian x 50% Haryana) were studied. Ascorbic acid, caffeine and chloroquine diphosphate were added in Tris-egg yolk-glycerol (control) extender at the concentration of 10 mM, 0.54 mM and 7 mM, respectively. The post-thaw semen parameters studied were structural (acrosomal integrity and livability), functional [motility, per cent sperm responding to hypo-osmotic swelling test, i.e. HOS positive sperm and penetrability of sperm in bovine cervical mucus, i.e. sperm penetration distance-SPD] and measurement of activities of Glutamic oxaloacetic transaminase (GOT), Glutamic pyruvic transaminase (GPT), Alkaline transaminase (AKP) and Acid transaminase (ACP). Certain enzymes in the seminal plasma. The semen from crossbred bull had significantly lower mass activity (P ≤ 0.01), initial progressive motility (P ≤ 0.01), sperm penetration distance (SPD value) (P ≤ 0.01), HOS positive sperm and seminal plasma GOT activity, but higher abnormal sperm. Incorporation of additives in the dilutor significantly improved the post-thaw semen quality, especially the post-thaw motility, post-thaw per cent live sperm, sperm penetration distance and sperm responding to hypo-osmotic solution. The leakage of all the 4 enzymes (GOT, GPT, ACP and AKP) was significantly less in the samples frozen in presence of ascorbic acid and chloroquine as compared to control. Ascorbic acid resulted in maximum improvement to the quality of frozen-thawed semen followed by chloroquine and caffeine. The study concludes that post-thaw quality of frozen semen of HF and crossbred bulls significantly improves if the dilutor is supplemented with 10 mM concentration of ascorbic acid.

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Effect of caffeine on the post-thaw motility of buffalo spermatozoa

Cited by 11 publications

References 10 publications

Effect of Different Types and Levels of Antioxidants on Viability and Acrosomal Status of Frozen-Thawed Spermatozoa of Buffalo Bulls.

Effect of Different Types and Levels of Antioxidants on Viability and Acrosomal Status of Frozen-Thawed Spermatozoa of Buffalo Bulls.

Effects of caffeine supplementation in post-thaw human semen over different incubation periods

Incorporation of Ascorbic Acid, Caffeine and Chloroquine Diphosphate in Dilutor Improves Structural and Functional Status of Frozen Semen

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