Effect of Caffeine on Increasing the Motility of Frozen Human Sperm

Barkay, J.; Zuckerman, H; Sklan, D.; Gordon, Shmuel

doi:10.1016/s0015-0282(16)42378-2

Cited by 44 publications

(15 citation statements)

References 10 publications

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“…The effect of caffeine 6 mM on sperm motility support those of previous studies by Eliasson (1975). However, in our hands 6 mM caffeine was ineffective when added to thawed spermatozoa from a cryobank though Barkay et al (1977) have recently reported that a higher concentration (7.2 mM) is effective. Barkay et al (1977) used a sodium citrate buffer cryoprotective medium, final glycerol ccncentration 5 0 i 0 (Barkay et al 1974).…”

Section: Discussioncontrasting

confidence: 53%

Effect of Two Methylxanthine Derivatives and Four Prostaglandins on the Motility of Spermatozoa from Volunteers and Oligozoospermic Patients

Read

Schnieden

1978

Int J of Andrology

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Caffeine has a dose dependant effect in vitro on the motility of human spermatozoa obtained from volunteers. In doses of 6 mM and 60 mM it delayed the fall in motility with time of spermatozoa when added within an hour of ejaculation. 6 mM caffeine had similar effects on the motility of spermatozoa obtained from oligozoospermic patients. However, 6 mM caffeine was ineffective in modifying the motility of previously cryopre‐served spermatozoa. In vitro theophylline 6 mM was effective in delaying the fall in motility with time of human spermatozoa obtained from volunteers and oligozoospermic patients within one h of ejaculation. In the doses used (prostaglandin E1, 25 μg/ml, E2, 25 μg/ml, F1α. 5 μg/ml and F2α, 5 μg/ml) none of the prostaglandins delayed the fall in in vitro motility with time of human spermatozoa obtained from volunteers or oligozoospermic patients, in fact prostaglandins E1, E2, and F2α caused an inhibition of the motility of spermatozoa obtained from volunteers.

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Section: Discussioncontrasting

confidence: 53%

Effect of Two Methylxanthine Derivatives and Four Prostaglandins on the Motility of Spermatozoa from Volunteers and Oligozoospermic Patients

Read

Schnieden

1978

Int J of Andrology

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“…Relative to the control incubations, caffeine induced~80% increase in motility at all dose levels, which is in good accordance with the 81% increase in motility induced by 5 mM-caffeine in the study of Schill et al (1979). Barkay et al (1977) examined the influence of caffeine dose on the increase in motility of cryostored spermatozoa and concluded that 7-2 mM was the optimum concentration for samples stored in excess of 30 days, although the statistical significance of this claim was not calculated. In the present study 5 mMcaffeine gave the highest mean increase in motility (88%) although this was not statistically different from the degree of stimulation observed with the 2 and 10 mM doses.…”

Section: Discussionmentioning

confidence: 99%

“…The possible use of caffeine to stimulate the motility of cryostored human spermatozoa has been suggested by the positive results obtained by Barkay, Zuckerman, Sklan & Gordon (1977) and Schill, Pritsch & Preissler (1979), although caffeine was found to be ineffective in this respect by Read & Schnieden (1978). Whether caffeine could also affect the ability of cryostored spermatozoa to penetrate cervical mucus and fertilize ova has not been fully explored.…”

Section: Introductionmentioning

confidence: 99%

Influence of caffeine on movement characteristics, fertilizing capacity and ability to penetrate cervical mucus of human spermatozoa

Aitken¹,

Best²,

Richardson³

et al. 1983

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Summary. When added to frozen\p=n-\thawedhuman semen, the 3 doses of caffeine tested (2, 5 and 10 mM) induced a significant increase in the percentage of motile spermatozoa but did not influence the quality of movement. Considerable variability was noted between samples in their responsiveness to caffeine which, at the 5 and 10 mM doses, was significantly correlated with the degree of motility lost during cryostorage.Caffeine treatment of frozen\p=n-\thawedhuman spermatozoa also increased the number of spermatozoa penetrating cervical mucus in unit time, by increasing the frequency rather than the success of collisions between spermatozoa and the cervical mucus interface. When caffeine-stimulated spermatozoa were washed free of seminal plasma containing this compound they were no longer at an advantage with respect to their motility or fertilizing ability. When 2 mM-caffeine was added to washed suspensions of capacitated spermatozoa it failed to stimulate motility but did significantly enhance the fertilizing ability of the spermatozoa, indicating a possible clinical role for this compound in in-vitro fertilization therapy.

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“…The procedure for the centrifugation of semen samples, the wash of pellet I and the deter mination of triple ATPase enzyme activities were in all respects in accordance with our previous works [6,7], which was used to determine ATPase activity. Barkay et al [1] reported that the optimal concentration of added caffeine would appear to be about 7.2 vaM, while in sam ples treated with either lower or higher caffeine concen trations no significant differences in motility were ap parent after 10 or 30 days of frozen storage. The differ ence between two amounts of added caffeine may result from the effect of postthaw or centrifugation.…”

Section: Methodsmentioning

confidence: 98%

The Effects of Caffeine and Theophylline on the Triple Adenosine Triphosphatase Enzyme Activities of Spermatozoa from Males with Oligoasthenospermia

Karacagil¹,

İmamoğlu²,

Paşaoğlu³

et al. 1990

Eur Urol

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Effect of Caffeine on Increasing the Motility of Frozen Human Sperm

Cited by 44 publications

References 10 publications

Effect of Two Methylxanthine Derivatives and Four Prostaglandins on the Motility of Spermatozoa from Volunteers and Oligozoospermic Patients

Effect of Two Methylxanthine Derivatives and Four Prostaglandins on the Motility of Spermatozoa from Volunteers and Oligozoospermic Patients

Influence of caffeine on movement characteristics, fertilizing capacity and ability to penetrate cervical mucus of human spermatozoa

The Effects of Caffeine and Theophylline on the Triple Adenosine Triphosphatase Enzyme Activities of Spermatozoa from Males with Oligoasthenospermia

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