Effect of cadmium on diaphorase activity and nitric oxide production in barley root tips

Valentovičová, Katarína; Halušková, Ľubica; Huttová, Jana; Mistrı́k, Igor; Tamás, Ladislav

doi:10.1016/j.jplph.2009.06.018

Cited by 69 publications

(35 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The function of NO may be different in various cell types and is probably dependent also on the severity and duration of stress conditions. It has previously been shown that a marked Cd-induced NO generation in pericycle, parenchymatic stellar cells and companion cells of protophloem of barley roots is associated with accelerated differentiation of root tips, which causes shortening of the root elongation zone and subsequent reduction in root growth during prolonged Cd-exposure (Valentovičová et al 2010). In contrast to these results, short-term exposure of roots to Cd-stimulated NO generation in the transition and elongation zone of root tip mitigating the Cd-induced root growth inhibition.…”

Section: Discussionmentioning

confidence: 99%

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

et al. 2015

Self Cite

View full text Add to dashboard Cite

Background and Aims The objective of this study was to analyse the role of nitric oxide (NO) in relation to reactive oxygen species (ROS) production and root growth inhibition in the early response of barley root tip to cadmium. Methods We used cell-permeable NO-binding dye DAF-2DA for the NO localization and cellimpermeable dye DAR-4M for the quantification of NO in barley root apex. Results A considerable increase in NO production was detected in the transition zone of root tips already 30 min after the immersion of barley roots into 15 μM Cd. This Cd-induced NO generation further increased during the recovery period and reached its maximum level 2 h after the short-term treatment. At this stage an enhanced Cd-induced NO generation was observed in the meristematic, transition and in the beginning of elongation zone in comparison with control roots. Inhibitor of NO synthase activity markedly reduced root growth in control seedlings and had a synergistic growth inhibitory effect with Cd. On the contrary, this Cdinduced root growth inhibition was markedly alleviated by the NO donor. Exposure of roots to Cd markedly increased the production of ROS in the proximal elongation zone of root. While NO did not affect ROS generation, inhibition of its synthesis slightly decreased ROS generation in both control and Cd-treated roots. Conclusions Our results revealed that enhanced NO production is a very early response of barley root to Cd stress and it is involved in the regulation of root growth mitigating Cd-induced root growth inhibition.

show abstract

Section: Discussionmentioning

confidence: 99%

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, the enhanced stress signal might be a result of higher levels of NO acting as a negative regulator of the resistance response in plants against metals. Recent reports revealed that endogenous NO generation contributes to Cd-induced root growth inhibition in plants (Valentovicova et al 2010;Ye et al 2013). Plant responses to Cu stress also involve shifts in NO levels, but the role of endogenous NO production remains incompletely understood.…”

Section: Discussionmentioning

confidence: 99%

Early generation of nitric oxide contributes to copper tolerance through reducing oxidative stress and cell death in hulless barley roots

2016

J Plant Res

View full text Add to dashboard Cite

The objective of this study was to investigate the specific role of nitric oxide (NO) in the early response of hulless barley roots to copper (Cu) stress. We used the fluorescent probe diaminofluorescein-FM diacetate to establish NO localization, and hydrogen peroxide (H2O2)-special labeling and histochemical procedures for the detection of reactive oxygen species (ROS) in the root apex. An early production of NO was observed in Cu-treated root tips of hulless barley, but the detection of NO levels was decreased by supplementation with a NO scavenger, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). Application of sodium nitroprusside (a NO donor) relieved Cu-induced root inhibition, ROS accumulation and oxidative damage, while c-PTIO treatment had a synergistic effect with Cu and further enhanced ROS levels and oxidative stress. In addition, the Cu-dependent increase in activities of superoxide dismutase, peroxidase and ascorbate peroxidase were further enhanced by exogenous NO, but application of c-PTIO decreased the activities of catalase and ascorbate peroxidase in Cu-treated roots. Subsequently, cell death was observed in root tips and was identified as a type of programed cell death (PCD) by terminal deoxynucleotidyl transferase dUTP nick end labeling assay. The addition of NO prevented the increase of cell death in root tips, whereas inhibiting NO accumulation further increased the number of cells undergoing PCD. These results revealed that NO production is an early response of hulless barley roots to Cu stress and that NO contributes to Cu tolerance in hulless barley possibly by modulating antioxidant defense, subsequently reducing oxidative stress and PCD in root tips.

show abstract

“…Nitric oxide (NO) might be one of the most promising candidates to mediate this process, since several studies have shown that Cd treatments can increase NO production in roots (Arasimowicz-Jelonek et al 2011;Besson-Bard et al 2009;De Michele et al 2009;Valentovičová et al 2011). Our study also confirmed such an accumulation of NO in Cd-treated roots (Fig.…”

Section: Discussionmentioning

confidence: 99%

Cadmium impairs iron homeostasis in Arabidopsis thaliana by increasing the polysaccharide contents and the iron-binding capacity of root cell walls

Lin

Lai

2015

Plant Soil

View full text Add to dashboard Cite

Background and aims Iron (Fe) deficiency is a wellknown symptom of cadmium (Cd) toxicity. Here, the mechanisms underlying Cd impairment of Fe homeostasis in Arabidopsis were investigated. Methods Arabidopsis plants were subjected to 0 (CK) or 10 μM Cd treatments. After the treatments period, Fe concentrations, expression levels of Fe uptake-and translocation-related genes, polysaccharide concentrations, pectin methylesterase activities (PME) and the degree of pectin methylation (DM) were analyzed.

show abstract

Effect of cadmium on diaphorase activity and nitric oxide production in barley root tips

Cited by 69 publications

References 51 publications

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

Enhanced nitric oxide generation in root transition zone during the early stage of cadmium stress is required for maintaining root growth in barley

Early generation of nitric oxide contributes to copper tolerance through reducing oxidative stress and cell death in hulless barley roots

Cadmium impairs iron homeostasis in Arabidopsis thaliana by increasing the polysaccharide contents and the iron-binding capacity of root cell walls

Contact Info

Product

Resources

About