Effect of Ca on Al-induced Activation of Antioxidant Enzymes in the Needles of Hinoki Cypress (<i>Chamaecyparis obtusa</i>)

Ogawa, Tadashi; Matsumoto, Chisato; Takenaka, Chisato; Tezuka, Takafumi

doi:10.1007/bf02762524

Cited by 10 publications

(5 citation statements)

References 22 publications

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“…While the stimulation in roots may be explained by the local impacts of Al on cell structure and function, the stimulation of antioxidant enzymes in leaf tissue suggests that the observed changes in plant nutrition alter leaf cell integrity or there may be some mechanism that transmits a stress signal from roots to leaves under Al exposure in the root or both. The increase in CAT activity in leaves of poplar due to rhizosphere Al stress is consistent with responses observed in Hinoki cypress (Ogawa et al 2000a(Ogawa et al , 2000b and in Norway spruce (Nagy et al 2004).…”

Section: Discussionsupporting

confidence: 86%

Rhizosphere carbon deposition, oxidative stress and nutritional changes in two poplar species exposed to aluminum

Naik¹,

Smith²,

Cumming³

2009

Tree Physiology

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Species and hybrids in the genus Populus have become the focus of investigation for use in biofuels production and their capacity to sequester carbon (C) in the environment. The identification of species resistant to marginal edaphic sites may be important in both of these endeavors. Plant growth, total dissolved organic carbon (TOC) and low molecular weight organic acid (OA) production, antioxidative enzyme activities and mineral content were assessed in Populus tremuloides L. and Populus trichocarpa Torr. & Gray seedlings under exposure to aluminum (Al). Both species were sensitive to Al, with significant reductions in shoot and root biomass at and above 50 microM Al. Exposure to Al induced 40-fold increases in TOC deposition in P. tremuloides and 100-fold increases in P. trichocarpa. In P. tremuloides, Al treatment induced root exudation of malic and citric acids, while Al increased exudation of citrate and oxalate in P. trichocarpa. Organic acids accounted for 20-64% of total C released upon Al exposure, with the proportion of OAs increasing in P. tremuloides and decreasing in P. trichocarpa. Dose-dependent responses of catalase and ascorbate peroxidase were observed in both root and leaf tissues, indicating that Al exposure induced oxidative stress in poplar. Treatment at and above 100 microM Al reduced the concentrations of calcium (Ca) and magnesium (Mg) in roots and leaves, whereas Al at or above 50 microM reduced root and leaf phosphorous (P) concentrations. The majority of Al taken up was retained in the root system. Even with the induction of OA exudation and accumulation, P. tremuloides and P. trichocarpa remained sensitive to Al, as evidenced by elevated antioxidative enzyme activities, which may reflect inhibition of Ca or P uptake and destabilization of cell homeostasis in these poplar species. Although plants exhibited reductions in growth and evidence of oxidative and nutritional stress, total C rhizodeposition rates for both species increased with increasing Al exposure concentration. Estimated C deposition rates of 16 mg C plant(-1) day(-1) were four-times larger than previously reported values for forest tree species, indicating that edaphic stress plays an important role in C flux to the rhizosphere.

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Section: Discussionsupporting

confidence: 86%

Rhizosphere carbon deposition, oxidative stress and nutritional changes in two poplar species exposed to aluminum

Naik¹,

Smith²,

Cumming³

2009

Tree Physiology

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“…Nevertheless, based on the results presented here the growth responses of tea roots to Al in the presence of other micronutrients was similar to that of the cells in simple salt solutions without other micronutrients. These results may cast in doubt the previous hypotheses that explained the beneficial effect of Al exclusively based on the interaction of Al with other micronutrients and alleviation of their toxicity (Konishi et al, 1985;Ogawa et al, 2000). The possible interaction of Al at high concentrations with other micronutrients however, cannot be excluded and is currently under investigation.…”

Section: Discussioncontrasting

confidence: 50%

“…A main protective role against ROS is attributed to SOD in catalyzing and dismutation of superoxide anions to O 2 and H 2 O 2 (Sreenivasulu et al, 2000) . The great difference between tea plant (and probably other Al-tolerant species, (Ogawa et al, 2000;Ezaki et al, 2001), and sensitive species (Cakmak and Horst, 1991), can be explained upon the upregulation of SOD followed by other ROS scavenging enzymes, in particular CAT and APX. In Al-sensitive plants, SOD activity is mainly triggered by Al that in turn results in dismutation of O 2 AE) and more production of another toxic species, H 2 O 2 .…”

Section: Plant Materialsmentioning

confidence: 98%

Effects of Aluminum on the Growth of Tea Plant and Activation of Antioxidant System

2005

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A possible connection between the effects of aluminum (Al) on the growth of tea plants and the active oxygen species scavenging system in root tips of intact tea plants and suspension-cultured tea cells was examined. Intact tea plants were treated with or without Al in a modified Hoagland solution, while suspension-cultured tea cells were treated with or without Al in a simple salt solution containing 3% sucrose and 0.2 mM calcium. Compared with the control treatments without Al, the activities of superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) increased by Al both in roots of intact plants and cultured cells. The level of peroxidation of membrane lipids, as well as the activity of wall-bound peroxidas, the content of lignin and wall-bound phenols, however, reduced by the treatment with Al either in tea roots or in cultured tea cells. The results indicated that Al-induced increase in the activities of antioxidant enzymes, resulting in increased membrane integrity and delayed lignification and aging, can be considered as a possible reason for the stimulatory effects of Al on the growth of the tea plants and this is irrespect of the presence of other micronutrients and their interaction with Al.Abbreviations: APX -ascorbate peroxidase; CAT -catalase; CPO -covalently bound peroxidase; IPOionically bound peroxidase; MDA -malondialdehyde; PAL -phenylalanine ammonia-lyase; PO -peroxidase; ROS -reactive oxygen species; SOD -superoxide dismutase; SPO -soluble peroxidase.

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“…This suggests that some mechanism for transmission of stress from roots to needles operated at Ca/Al ratios of 0.16 (group 4) and 1.0 (group 3) within 3 days of applying the high Al solution to the roots. The quick increase in SOD activity in needles due to rhizosphere Al stress is consistent with the early response to Al stress observed by other authors (Ogawa et al 2000a(Ogawa et al , 2000b. However, Al itself was probably not transferred from roots to needles.…”

Section: Discussionsupporting

confidence: 88%

“…Catalase converts hydrogen peroxide to H 2 O and O 2 (Scandalios 1993). Activity levels of these two enzymes have been used to evaluate plant stress (Inaba et al 1998;Ogawa et al 1998Ogawa et al , 2000aOgawa et al , 2000b. The purpose of this study was to investigate the mitigation of Al toxicity by Ca and Mg in seedlings of Japanese cedar (Cryptomeria japonica D. Don).…”

mentioning

confidence: 98%

Mitigation of aluminum toxicity by calcium and magnesium in Japanese cedar (Cryptomeria japonica)

Takami

Takenaka

Tezuka

2005

Journal of Forest Research

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We investigated the short-term and long-term mitigation of Al toxicity by Ca and Mg in pot trials of Japanese cedar (Cryptomeria japonica D. Don). We found that in the initial stages of treatment, Al toxicity at high Al concentration (5 mM) was mitigated by Ca and Mg through the stimulation of antioxidant enzyme activities in needles. However, growth reduction occurred after 11 months' exposure to Al despite the coexistence of Ca and Mg. Growth reduction was related to Al 3ϩ activity in solution rather than the concentration of Ca and Mg. Therefore, when considering the influence of soil acidification on Al toxicity in forest ecosystems, it is important to consider not only the potential for mitigation of Al toxicity by base cations, but also the potential for factors in the soil solution to change the chemical form of Al.

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Effect of Ca on Al-induced Activation of Antioxidant Enzymes in the Needles of Hinoki Cypress (Chamaecyparis obtusa)

Cited by 10 publications

References 22 publications

Rhizosphere carbon deposition, oxidative stress and nutritional changes in two poplar species exposed to aluminum

Rhizosphere carbon deposition, oxidative stress and nutritional changes in two poplar species exposed to aluminum

Effects of Aluminum on the Growth of Tea Plant and Activation of Antioxidant System

Mitigation of aluminum toxicity by calcium and magnesium in Japanese cedar (Cryptomeria japonica)

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