Effect of C60 fullerene nanoparticles on the diet-induced obesity in rats

Halenova, Tetiana; Raksha, Nataliia; Vovk, Tetiana; Savchuk, Olexiy; Li, Ostapchenko; Prylutskyy, Yu. І.; Kyzyma, O. A.; Ritter, Uwe; Scharff, P.

doi:10.1038/s41366-018-0016-2

Cited by 36 publications

(24 citation statements)

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“…Since oxidative stress is a major pathophysiological factor in the development of obesity and the progression of obesity-related complications, we hypothesized that DDW may also alleviate these conditions. To test this hypothesis, we used a rat DIO model [34,37,52]. To determine the effect of DDW on nutritional and metabolic parameters, rats were fed either a normal diet or a high-fat diet for 56 days and had ad libitum access to DDW (instead of MilliQ water) from the 35th day from the start of the experiment.…”

Section: Discussionmentioning

confidence: 99%

“…Obesity is a pathological state closely related to the impairment of glucose metabolism and insulin resistance development [2,34,35]. Indeed, as depicted in Table 1, the high-fat diet led to a 1.4-fold increase in blood glucose concentration (p < 0.05) as well as a 2.5-fold decrease in serum insulin level (p < 0.05) compared to the same parameters in the control rats.…”

Section: Biochemical Parameters At the End Of The Experiments In Contrmentioning

confidence: 90%

“…The diet-induced obesity (DIO) group (n = 20) was fed a high-fat diet (38.8% fat, 15.5% protein and 45.7% carbohydrate, 28.71 kJ·g −1 ). Normal rat chow was supplemented to create DIO food as follows: 60% standard chow, 10% pork fat, 10% eggs, 9% sucrose, 5% dry milk, 5% peanuts and 1% vegetable oil [34,52].…”

Section: Experimental Designmentioning

confidence: 99%

“…The concentrations of insulin and cytokines (interferon gamma (IFNγ), interleukins (ILs) -1β, -6, -4, -10 and transforming growth factor-alpha (TNFα)) were measured in serum by enzyme-linked immunosorbent assay following standard protocols. Detailed description of the methods was presented in a previous study [34]. Samples were contained in 96-well ELISA plates (Thermo Fisher Scientific, Waltham, MA, USA), the corresponding primary antibodies (Santa Cruz Biotechnology, Inc., USA), horseradish peroxidase (Sigma-Aldrich, St. Louis, MO, USA).…”

Section: Biochemical Analysismentioning

confidence: 99%

“…Measurements were performed on a spectrophotometer Smart SpecTMPlus (BioRad, Hercules, CA, USA). SOD activity was measured by a method [79] based on the capability of the enzyme to inhibit the autooxidation of adrenaline (epinephrine) [34]. Catalase activity was measured by a previously described method [80].…”

Section: Biochemical Analysismentioning

confidence: 99%

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Deuterium-Depleted Water as Adjuvant Therapeutic Agent for Treatment of Diet-Induced Obesity in Rats

et al. 2019

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In this study, we present the potential application of deuterium-depleted water (DDW) for the prevention and adjuvant treatment of obesity in rats. We tested the hypothesis that DDW can alleviate diet-induced obesity (DIO) and its associated metabolic impairments. Rats fed a high-fat diet had an increased body weight index (BWI), glucose concentration, and level of certain proinflammatory cytokines; decreased levels of insulin in the serum; decreased tryptophan and serotonin in the brain, and a decreased concentration of some heavy metals in the liver. Drinking DDW at a concentration of 10 ppm deuterium/protium (D/H) ad libitum for 3 weeks restored the BWI, glucose (serum), tryptophan (brain), and serotonin (brain) levels and concentration of Zn in the liver in the DIO animals to those of the controls. The levels of proinflammatory cytokines (IL-1β, IL-6, IFNγ) and anti-inflammatory TNFα were decreased in DIO rats, while anti-inflammatory cytokine (IL-4, IL-10) levels remained at the control levels, which is indicative of a pathophysiological syndrome. In contrast, in groups of rats treated with DDW, a significant increase in anti-inflammatory (IL-4, IL-10) and proinflammatory cytokines (IFNγ) was observed. This finding indicates a reduction in systemic inflammation in obese animals treated with DDW. Similarly, the high-fat diet caused an increased level of oxidative stress products, which was accompanied by decreased activity of both superoxide dismutase and catalase, whereas the administration of DDW decreased the level of oxidative stress and enhanced antioxidant enzyme activities.Molecules 2020, 25, 23 2 of 15 the development of new, safer and more effective pharmaceutical approaches for obesity treatment is essential.Natural water is a mixture of molecules containing stable isotopes 16 O, 17 O, 18 O, 1 H, and 2 H. The ratio of deuterium atoms to protium (D/H) in fresh and marine water is 132-156 µg/g (ppm) [7,8].Since the mass of protium H is half of the mass of deuterium D, effects arising from the replacement of isotopes influence biological systems; for example, the rates of chemical reactions between substances containing these isotopes may differ by a factor of 5-10 [9-11].Numerous publications over the last 20 years have been devoted to the study of the role of deuterium in natural (150 ppm) or reduced content in the human body [12]. It has been shown that deuterium-depleted water (DDW) exhibits antidotal properties with individual and combined effects on the dosage of pharmaceutical substances and auxiliary substances [13][14][15][16]. The mechanisms of such influence are due to the structure, physicochemical properties of DDW [17] and changes in ligand-receptor interactions in biological objects of different hierarchical levels [18]. DDW increases the rate of photosynthesis and promotes the growth of plants and aquatic animals [19,20]. In Europe, the USA, Japan, China and Russia, DDW is used for prophylactic and therapeutic purposes [21,22]. Currently, DDW is using as an adjuvant in cancer treatment ...

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Section: Discussionmentioning

confidence: 99%

Section: Biochemical Parameters At the End Of The Experiments In Contrmentioning

confidence: 90%

Section: Experimental Designmentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

Section: Biochemical Analysismentioning

confidence: 99%

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