Effect of C-reactive protein on the production of nitric oxide by mouse peritoneal macrophages

Abstract

C-reactive protein in concentrations of 1-20 mg/ml induces the production of nitrogen oxide by mouse peritoneal macrophages attracted by peptone solution. Protein kinases A and C and RNA and protein production inhibitors abolish this effect, while inhibitors of the lipoxigenase and cycloxigenase pathways of arachidonic acid metabolism attenuate it.

Changes in Superoxide Dismutase Activity and Peroxynitrite Content in Rat Peritoneal Macrophages Exposed to He-Ne Laser Radiation

“…Nitric oxide production was deter mined according to the content of NO 2 -ions in the cell incubation medium by the Griss method described by Shebzukhov et al [15] and Ding et al [16]. A sample for determination of NO 2 -concentration contained about 1 ml of distilled water, 150 µl of culture medium, 75 µl of reagent A (1.5% sulfanilamide in 1 N HCl), and 75 µl of reagent B (0.15% naphthylethylenediaminechloride in distilled water).…”