Effect of Buffer and pH on Detergent-Assisted Foam Fractionation of Cellulase

Abstract

Foam fractionation can be used to concentrate hydrophobic proteins (such as bovine serum albumin) in solution. However, it cannot be used directly to concentrate cellulase because cellulase solution alone produces only a small amount of foam when aerated. The addition of a surfactant to a cellulase solution makes it possible to foam nonfoaming proteins, such as cellulase. In this paper, three surfactants, namely, cetyltrimethylammonium bromide (CTAB, a cationic detergent), sodium dedecyl sulfate (SDS, an anionic detergent), and pluronic F-68 (a nonionic detergent), were individually added to a 200 mg/L cellulase solution and then aerated. The addition of these surfactants increased the amount of foam produced and enhanced the volume of foamate, the collapsed foam product. The detergent CTAB exhibited the highest enrichment and mass recovery ratios of the three detergents tested. Small changes in the pH in the cellulase foam fractionation process had a strong effect on the cellulase enrichment ratio. At an air flow rate of 4 mL/min, the enrichment ratio at pH 5.0 was 2.7, whereas the enrichment ratios at pH 4.8, 5.2, and 5.5 were less than 2.0. Increases in the buffer concentration resulted in increases in the mass recovery and decreases in the enrichment ratio.

Recovery of surfactant SDS and Cd2+ from permeate in MEUF using a continuous foam fractionator

“…There have been many studies on foam fractionation including the effect of operating mode [9,10] and foam properties [11,12]. Foam fractionation has been extensively studied for the purpose of removing pollutants such as heavy metal ions and proteins from water by adding surfactant [13][14][15][16]. Rubin et al [17] investigated the removal of lead (II) using sodium lauryl sulfate as a collector.…”

Isolation of soy whey proteins from isoflavones in the concentrated solution using foam fractionation

“…Interfacial adsorption and foam drainage are two important processes affecting the performances of foam fractionation. Some experiments had demonstrated that the surface excess concentration of a protein was the highest at its pI [32][33][34]. However, the maximal enrichment ratio of WSP did not occur at the range of pH from 4.5 to 6.0.…”

Study on Riboflavin Recovery from Wastewater by a Batch Foam Separation Process

“…Foam separation is a simple and low-cost method, belonging to the adsorptive bubble separation techniques (9). It has been extensively studied for the purpose of removing pollutants such as heavy metal ions and proteins from water by adding surfactant (10)(11)(12)(13). This paper investigates foam separation of riboflavin from industrial simulation wastewater solution.…”