Effect of BSA on carbon nanotube dispersion for<i>in vivo</i>and<i>in vitro</i>studies

Elgrabli, Dan; Abella-Gallart, Steve; Aguerre-Chariol, Olivier; Robidel, Franck; Rogerieux, Françoise; Boczkowski, Jorge; Lacroix, Ghislaine

doi:10.1080/17435390701775136

Cited by 63 publications

(64 citation statements)

References 26 publications

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“…Nanomaterial exposure media often contains biomolecules such as globular protein BSA (Elgrabli et al, 2007; Qiu et al, 2010; Saleh et al, 2010) and a more complex enzymetic and protein mixture of FBS (Hu et al, 2011) at different concentrations. Understanding the aggregate structure in presence of these essential biomolecular additives will likely provide further insight onto SWNT aggregation and its impact to biological responses (Pasquini et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Fractal structures of single-walled carbon nanotubes in biologically relevant conditions: Role of chirality vs. media conditions

et al. 2013

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Aggregate structure of covalently functionalized chiral specific semiconducting single-walled carbon nanotubes (SWNTs) was systematically studied employing static light scattering (SLS). Fractal dimensions (Df) of two specific chirality SWNTs—SG65 and SG76 with (6, 5) and (7, 6) chiral enrichments—were measured under four biological exposure media conditions, namely: Dulbecco’s Modified Eagle Medium (DMEM), Minimum Essential Medium (MEM), Roswell Park Memorial Institute (RPMI) 1640 medium, and 0.9% saline solution. The SWNTs exhibited chiral dependence on Df with SG65 showing more fractal or loosely bound aggregate structures, i.e., lower Df values (range of 2.24 ± 0.03 to 2.64 ± 0.05), compared to the SG76 sample (range of 2.58 ± 0.13 to 2.90 ± 0.08). All the Df values reported are highly reproducible, measured from multiple SLS runs and estimated with ‘random block-effects’ statistical analysis that yielded all p values to be <0.001. The key mechanism for such difference in Df between the SWNT samples was identified as the difference in van der Waals (VDW) interaction energies of these samples, where higher VDW of SG76 resulted in tighter packing density. Effect of medium type showed lower sensitivity; however, presence of di-valent cations (Ca2+) in DMEM and MEM media resulted in relatively loose or more fractal aggregates. Moreover, presence of fetal bovine serum (FBS) and bovine serum albumin (BSA), used to mimic the in vitro cell culture condition, reduced the Df values, i.e., created more fractal structures. Steric hindrance to aggregation was identified as the key mechanism for creating the fractal structures. Also, increase in FBS concentration from 1% to 10% resulted in increasingly lower Df values.

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Section: Resultsmentioning

confidence: 99%

Fractal structures of single-walled carbon nanotubes in biologically relevant conditions: Role of chirality vs. media conditions

et al. 2013

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“…However, the presence of proteins were reported to stabilize the suspension of CNT (Sager et al, 2007;Elgrabli et al, 2007). Since DLS technique has an upper limit of 6 µm, the presence of larger aggregates was evaluated by optical microscopy ( Figure 2b).…”

Section: Dispersion Of Mwcnt In Cellular Mediamentioning

confidence: 99%

The Role of Iron Impurities in the Toxic Effects Exerted by Short Multiwalled Carbon Nanotubes (MWCNT) in Murine Alveolar Macrophages

Aldieri

Fenoglio

Cesano

et al. 2013

Journal of Toxicology and Environmental Health, Part A

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Lung toxicity mediated by multiwalled carbon nanotubes (MWCNT) has been widely demonstrated and recently associated with induction of carcinogenic asbestos-like effects, but the chemical features which drive this toxic effect have still not been well elucidated. The presence of metals as trace contaminants during MWCNT preparation, in particular iron (Fe) impurities, plays an important role in determining a different cellular response to MWCNT.Our goal was to clarify the mechanisms underlying MWCNT toxicity in correlation to the presence of Fe impurities by exposing murine alveolar macrophages to two different MWCNT samples, which differ only for the presence or absence of Fe. Data show that only Fe-rich MWCNT were significantly cytotoxic, genotoxic and induced a potent cellular oxidative stress, while Fe-free MWCNT did not exert any of these toxic effects. These results confirm that Fe content represents a very important key constituent in promoting MWCNT induced toxicity, and this needs to be taken into consideration when planning new, safer, preparation routes.Running Title: The role of impurities in MWCNT toxicity.

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“…Pristine SWCNT were well-dispersed in NaCl in the presence of bovine serum albumin after at least 20 minutes sonication in an ultrasonic bath. 19 Functionalized SWCNT were purchased from Sigma-Aldrich (Lyon, France). They have an individual diameter of 1 to 2 nm and an individual length of 0.5 to 2 µm.…”

Section: Nanoparticle Suspensionmentioning

confidence: 99%

In vivo biodistribution and biological impact of injected carbon nanotubes using magnetic resonance techniques

Faraj¹,

Fauvelle²,

Luciani³

et al. 2011

IJN

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International audienceSingle-walled carbon nanotubes (SWCNT) hold promise for applications as contrast agents and target delivery carriers in the field of nanomedicine. When administered in vivo, their biodistribution and pharmacological profile needs to be fully characterized. The tissue distribution of carbon nanotubes and their potential impact on metabolism depend on their shape, coating, and metallic impurities. Because standard radiolabeled or fluorescentlylabeled pharmaceuticals are not well suited for long-term in vivo follow-up of carbon nanotubes, alternative methods are required

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Effect of BSA on carbon nanotube dispersion forin vivoandin vitrostudies

Cited by 63 publications

References 26 publications

Fractal structures of single-walled carbon nanotubes in biologically relevant conditions: Role of chirality vs. media conditions

Fractal structures of single-walled carbon nanotubes in biologically relevant conditions: Role of chirality vs. media conditions

The Role of Iron Impurities in the Toxic Effects Exerted by Short Multiwalled Carbon Nanotubes (MWCNT) in Murine Alveolar Macrophages

In vivo biodistribution and biological impact of injected carbon nanotubes using magnetic resonance techniques

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