Effect of broth dilution and its application in josamycin fermentation

Eiki, Hideo; Gushima, Hiroshi; Saito, Takeshi; Osono, Takashi

doi:10.1016/0922-338x(90)90280-a

Cited by 4 publications

(4 citation statements)

References 6 publications

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“…P6621 U-12-2 using fish meal (data not shown) or gluten meal as the nitrogen source demonstrated high apparent viscosity in culture broth (3,6). Results from these previous reports are in accord with the concept that filamentous grown mycelia are responsible for high apparent viscosity.…”

Section: Discussionsupporting

confidence: 88%

“…Gbewonyo and Wang (9) showed that the limitation of dissolved oxygen in Penicillum chrysogenum culture could restrict the growth of immobilized mycelia in Celite beads. Eiki et al (6) reported that the dilution of culture with sterilized water in josamycin production was very effective not only for obtaining high production yield but also for maintaining a lower apparent viscosity during the cultivation of S. narbonensis var jasamyceticus in a jar fermentor. Park et al (18) proposed that partial immobilization of the mycelia on cellulose was one method to reduce apparent viscosity in the culture of S. fradiae in an airlift bioreactor.…”

Section: Discussionmentioning

confidence: 99%

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Dependence of Apparent Viscosity on Mycelial Morphology of Streptomyces fradiae Culture in Various Nitrogen Sources

Choi

Park

Okabe

2000

Biotechnology Progress

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To examine what causes increased viscosity in culture broth in Streptomyces fradiae culture, various natural nitrogen sources were investigated. Extracellular protease activity increased with culture time and decomposed the natural nitrogen source into amino acids. In the case of gluten meal, after a culture time of 5 d, concentrations of glutamic acid and aspartic acid had increased to 600 and 200 mg/L, respectively, which were about 3- and 2-fold as high as levels in cultures under similar conditions using Pharmamedia. For various amino acids tested, the addition of glutamic acid or aspartic acid mixture to the culture medium raised the apparent viscosity to its highest demonstrated value, 260 mPa.s after 5 d of culture, which was 3-fold higher than without amino acids. Consumption of the decomposed glutamic acid and aspartic acid was dependent on the activities of glutamate dehydrogenase and aspartate aminotransferase, respectively. When ammonium ion was used as the nitrogen source, cell concentration reached 1.75 g/L measured as an intracellular nucleic acid concentration, which was about 2.3-fold higher than that with any other natural nitrogen source. However, apparent viscosity was only 75 mPa.s, a value one-third that of the amino acid mixture, and 70% of the pellets were bigger than 1.2 x 10(4) microm(2). In the case of gluten meal or the amino acid mixture, pellets bigger than 1.2 x 10(4) microm(2) comprised only 8%. This demonstrates that consumption of some amino acids affected the formation of filamentous morphology, which caused an increase in the apparent viscosity of the culture broth, and the apparent viscosity was not caused by the mycelial concentration but the mycelial morphology.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Dependence of Apparent Viscosity on Mycelial Morphology of Streptomyces fradiae Culture in Various Nitrogen Sources

Choi

Park

Okabe

2000

Biotechnology Progress

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“…Recently, we found that the culture of cephamycin‐producing Streptomyces sp. P6621 U‐12‐2 using fish meal (data not shown) or gluten meal as the nitrogen source demonstrated high apparent viscosity in culture broth (3, 6). Results from these previous reports are in accord with the concept that filamentous grown mycelia are responsible for high apparent viscosity.…”

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Glycaemic control with advanced new insulins for the treatment of diabetes

Day

2006

European Diabetes Nursing

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The benefits of maintaining blood glucose levels as near normal as possible are well appreciated. The advent of insulins with varying rates of onset and duration of action has made it possible to closely mimic normal physiological glycaemic control.This review considers advances in insulin therapy with attention focussed on the most recently introduced rapid-acting insulin analogue glulisine (Apidra ® ) and the novel inhaled insulin Exubera ® .

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A dense cell retention culture system using stirred ceramic membrane reactor

Suzuki

Sato²,

Kominami³

1994

Biotech & Bioengineering

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A novel reactor design incorporating porous ceramic tubes into a stirred jar fermentor was developed. The stirred ceramic membrane reactor has two ceramic tubular membrane units inside the vessel and maintains high filtration flux by alternating use for filtering and recovering from clogging. Each filter unit was linked for both extraction of culture broth and gas sparging. High permeability was maintained for long periods by applying the periodical control between filtering and air sparging during the stirred retention culture of Saccharomyces cerevisiae. The ceramic filter aeration system increased the k(L)a to about five times that of ordinary gas sparing. Using the automatic feeding and filtering system, cell mass concentration reached 207 g/L in a short time, while it was 64 g/L in a fed-batch culture. More than 99% of the growing cells were retained in the fermentor by the filtering culture. Both yield and productivity of cells were also increased by controlling the feeding of fresh medium and filtering the supernatant of the dense cells culture. (c) 1994 John Wiley & Sons, Inc.

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Effect of broth dilution and its application in josamycin fermentation

Cited by 4 publications

References 6 publications

Dependence of Apparent Viscosity on Mycelial Morphology of Streptomyces fradiae Culture in Various Nitrogen Sources

Dependence of Apparent Viscosity on Mycelial Morphology of Streptomyces fradiae Culture in Various Nitrogen Sources

Glycaemic control with advanced new insulins for the treatment of diabetes

A dense cell retention culture system using stirred ceramic membrane reactor

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