Effect of broodstock diets on lipid classes and their fatty acid composition in eggs of gilthead sea bream (Sparus aurata L.)

Abstract

Total lipid content, total lipid fatty acids, lipid classes and their fatty acid compositions were studied in eggs of gilthead sea bream from two different broodstocks.The two broodstock diets used had the same total lipid content, but differed with respect to their fatty acid levels. The lipid class composition of eggs from different origins was not significantly different. The predominant polar lipids were phosphatidylcholine, phosphatidylethanolamine and sphingomyelin, and the main neutral lipid classes were cholesterol esters and triacylglycerols.The fatty acid composition of total lipids and main lipid classes in the two groups of eggs showed marked differences, reflecting the influence of fatty acid levels in the broodstock diets.

Metabolism and Functions of Lipids and Fatty Acids in Teleost Fish

“…Marine fish eggs with relatively low levels of lipid include those from herring, haddock, whiting (Merlangus merlangus), saithe (Pollachius virens) (Tocher and Sargent, 1984b), cod (Fraser et al, 1988) and halibut (Falk-Petersen et al, 1989), but freshwater species such as perch (Perca fluviatilis), northern pike (Esox lucius), tilapia and roach (Leucisus rutilis) also have low lipid eggs (Henderson and Tocher, 1987). In contrast, eggs from salmon, rainbow trout, striped bass and whitefish (Coregonus albula) have higher lipid contents, and eggs from the marine fish gilthead sea bream (Mourente and Odriozola, 1990;Ronnestad et al, 1994), Senegal sole (Solea senagalensis) (Vazquez et al, 1994), common dentex (Dentex dentex) (Mourente et al, 1999a), sea bass (Ronnestad et al, 1998) and turbot (Silversand et al, 1996) all have higher levels of neutral lipids (>50% of total lipid), with the eggs from all these species having oil globules similar to those found in the relatively lipid -rich eggs of sand eel (Ammodytes lancea) and capelin (Tocher and Sargent, 1984b). The polar lipids of most fish eggs are dominated by phosphoglycerides, particularly PtdCho, followed by PtdEtn, PtdSer and PtdIns.…”

Effect of broodstock diets on total lipids and fatty acid composition of larvae of gilthead sea bream (Sparus aurata L.) during yolksac stage

“…The two broodstocks (named FI and NC), their respective different diets and the feeding pattern used in this study were the same as described in the previous paper (Mourente and Odriozola 1989).…”

“…The eggs were produced naturally as described previously (Mourente and Odriozola 1989). Floating viable eggs were placed in cylindrical egg incubators of PVC and 0.5 m of diameter with two small feeding tubes of running sea water, placed diametrically opposed to one another to oxygenate the water, and produce a gentle circular current inside the incubator tank.…”

Effect of diet (fatty acid and protein) content during spawning season on fertility, eggs and larvae quality of common porgy (Pagrus pagrus, Linnaeus 1758)

“…In some fish species, LC-PUFA (Long chain polyunsaturated fatty acids) in broodstock nutrition increase fertility, fertilization and egg quality. This has been described in species such as sea bream, Sparus aurata (Mourente and Odriozola, 1990, Fernandez-Palacios et al, 1995, halibut, Hippoglossus hipoglossus (Bruce et al, 1993;Mazorra et al, 2003), sea bass, Dicentrarchus labrax (Cerdá et al, 1994b;Carrillo et al, 1995;Navas et al, 1997), Japanese sea bream, Pagrus major (Watanabe et al, 1984a,b,c,d;1985 a,b;1991), trout rainbow, Oncorhynchus mykis (Washburn et al, 1990;Choubert and White, 1993;Choubert et al, 1998) Atlantic salmon, Salmo salar (Eskelinen, 1989;Christiansen and Torrissen, 1997), coho salmon, Oncorhynchus kisutch (Hardy et al, 1984;, tilapia, Oreochromis niloticus (Cumaratunga and Mallika, 1991;Gunasekera et al, 1995Gunasekera et al, , 1996aGunasekera et al, , b, 1997Siddiqui et al, 1998), common carp Cyprinus carpio (Manissery et al, 2001)Japanese flounder, Paralichthys olivaceus (Furuita et al, 2003, ) yellowtail Seriola quinqueradiata (Agius et al, 2001), Senegal sole, Solea senegalensis (Dinis et al, 1999), milkfish, Chanos chanos (Emata et al, 2000), turbot, Scophthalmus maximus (Lavens et al, 1999), and the catfish, Ictalurus punctatus (Torrans and Lowell, 2001). The aim of this study is to determine how food quality of broodstock could affect fertility and egg quality.…”