Effect of breed on the expression of Sirtuins (Sirt1-7) and antioxidant capacity in porcine brain

Abstract

Sirtuins, NAD-dependent histone deacetylase (HDAC), are correlated to aging and antioxidant. The aim of this study was to determine breed differences of porcine Sirtuins expression and antioxidant capacity in brain between Jinhua pigs (a fatty breed of China) and Danish Landrace pigs (a leaner breed). Effect of age on Sirtuins' expression was also investigated. At the age of 180 days, the mRNA levels of Sirt1, as well as Sirt2 and Sirt4, in Jinhua pigs were greater, but the mRNA levels of Sirt3, Sirt5, Sirt6, and Sirt7 of Jinhua pigs were lower compared with Danish Landrace pigs. Likewise, at the same BW of 64 kg, the mRNA levels of Sirtuins, except Sirt5 and Sirt7, in Jinhua pigs were greater than Danish Landrace pigs. Meanwhile, Jinhua pigs possessed higher antioxidants activity than Danish Landrace pigs either at the same age or at the same BW. Furthermore, mRNA levels of Sirtuins were decreased with age in brain of the two breeds from 30 to 120 days. The results indicated that Sirtuins expression in brain was different between fatty and lean pigs, and Sirtuins expression may be correlated to antioxidant capacity. In addition, age could down-regulate Siruins expression in porcine brain.

Segmental Aging Underlies the Development of a Parkinson Phenotype in the AS/AGU Rat

“…Typically, respective sirtuin expression decreases with age, with the exception of Sirt7 [22,55,56]. This reduction may be indicative of DNA damage accumulation and may be a precursor to apoptosis when damage is too great to repair.…”

Changes in human sirtuin 6 gene promoter methylation during aging

“…However, Ren et al (17) demonstrated that SIRT6 mRNA levels in porcine brain decreased with age, which is an indicator of a possible increase in promoter methylation. By contrast, SIRT6 protein levels were found to be increased during aging in diabetic mice (18) and following exercise in young and older mice (19).…”

“…This study included 55 individuals (34 females and 21 males), aged 9-95 years, divided into 8 age groups with 10-year intervals, with the exception of the 1st (9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) and the 8th groups (80-95 years). Blood samples were collected at the Istanbul University Medico-Social Center, Turkey, with the written consent of the individuals who completed a form indicating their background regarding gender, age, inherited diseases, cancer, exposure to chemical or radioactive agents, permanent use of medicines, smoking habits and presence in the family of individuals aged >90 years.…”

Sirtuins and Their Roles in Brain Aging and Neurodegenerative Disorders

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