Effect of Breast Cancer and Adjuvant Therapy on Adipose-Derived Stromal Cells: Implications for the Role of ADSCs in Regenerative Strategies for Breast Reconstruction

Challapalli, Ritihaas Surya; Dwyer, Róisín M.; McInerney, Niall; Kerin, Michael J.; Lowery, Aoife

doi:10.1007/s12015-020-10038-1

Cited by 6 publications

(7 citation statements)

References 142 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo and vitro, Cordelier observed that ADSCs may impede tumor cell proliferation by disrupting the tumor cell cycle in pancreatic cancer [14]. Additionally, Azuma proposed in their experiment that ADSCs may mediate the inhibition of prostate cancer through signaling pathways involving TGF-β signaling pathway [35]. Our contribution to this eld sheds light on an alternative perspective, elucidating the anti-tumor effect of ADSCs by impeding tumor cell growth through recombinant tumor metabolism.…”

Section: Discussionmentioning

confidence: 74%

Single-cell RNA sequencing has unveiled the contribution of ADSCs in reprogramming metabolism in triple-negative breast cancer

Zhang,

Xiao,

Zhang

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: Mesenchymal stem cells (MSCs) constitute a significant element of the tumor microenvironment (TME), exerting dynamic influences on various tumors through mechanisms such as chemotaxis, interaction with immune cells. Therefore, MSCs have the ability to dynamically affect various types of tumors. The purpose of this study was to determine the impact of three common MSCs on tumors using single-cell analysis technology. Methods: We accessed single-cell sequencing date for mesenchymal stromal stem cells from adipose tissue (ADSCs), human bone marrow (BMSCs), and stem cells from human exfoliated deciduous teeth (SHED) from GEO database (GSE157863). We utilized R packages, including Seurat and Rtsne, for analyzing difference between ADSCs and other MSCs. Subsequently, ADSCs were injected into nude mice bearing mammary tumor cells, and PET-CT was conducted to compare metabolic absorption rates before and after injection. Additionally, bioinformatic analysis and Immunohistochemistry (IHC) were utilized to investigate the function and distribution of CCDC80. Results: Bioinformatic analysis revealed that ADSCs differed from BMSCs and SHED, suggesting a potential inhibition of tumor growth through alterations in glycine metabolism. In vivo experiments using a triple-negative breast cancer model demonstrated that the injection of ADSCs inhibited tumor growth. Furthermore, our findings implicated CCDC80 as a potentially pivotal gene influencing metabolism. Conclusion: The data derived from this study indicate that ADSCs possess unique capabilities to modulate metabolism and suppress tumor growth. Consequently, ADSCs may emerge as a promising cellular treatment option for triple-negative breast cancer, a subtype currently lacking effective therapeutic interventions.

show abstract

Section: Discussionmentioning

confidence: 74%

Single-cell RNA sequencing has unveiled the contribution of ADSCs in reprogramming metabolism in triple-negative breast cancer

Zhang,

Xiao,

Zhang

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…These newly discovered pathways of resistin may be mediated through ADSCs in the breast tumor microenvironment via CXCL5 secretion, leading to the malignant behaviors of breast cancer cells. This may have implications not only on that the resistin-ADSC-CXCL5 pathway as a therapeutic target, but on the re-evaluation of factors that may affect ADSC activities in the tumor microenvironment, such as neoadjuvant and adjuvant therapies, and post-surgical autologous fat grafting in breast cancer patients 47 . Furthermore, the co-culture model of ADSCs with breast cancer cell lines reported in this study may be applied to investigate the impact of ADSCs on patient-derived tumor xenografts (PDXs) to recapitulate the complexity of the breast tumor microenvironment 48 .…”

Section: Discussionmentioning

confidence: 99%

ADSCs stimulated by resistin promote breast cancer cell malignancy via CXCL5 in a breast cancer coculture model

Hung

et al. 2022

Sci Rep

View full text Add to dashboard Cite

The tumor microenvironment represents one of the main obstacles in breast cancer treatment owing to the presence of heterogeneous stromal cells, such as adipose-derived stem cells (ADSCs), that may interact with breast cancer cells and promote cancer development. Resistin is an adipocytokine associated with adverse breast cancer progression; however, its underlying mechanisms in the context of the breast tumor microenvironment remain largely unidentified. Here, we utilized a transwell co-culture model containing patient-derived ADSCs and breast cancer cell lines to investigate their potential interaction, and observed that breast cancer cells co-cultured with resistin-treated ADSCs (R-ADSCs) showed enhanced cancer cell growth and metastatic ability. Screening by proteome arrays revealed that C-X-C motif chemokine ligand 5 (CXCL5) was released in the conditioned medium of the co-culture system, and phosphorylated ERK was increased in breast cancer cells after co-culture with R-ADSCs. Breast cancer cells treated with the recombinant proteins of CXCL5 showed similarly enhanced cell migration and invasion ability as occurred in the co-culture model, whereas application of neutralizing antibodies against CXCL5 reversed these phenomena. The orthotopic xenograft in mice by breast cancer cells after co-culture with R-ADSCs had a larger tumor growth and more CXCL5 expression than control. In addition, clinical analysis revealed a positive correlation between the expression of resistin and CXCL5 in both tumor tissues and serum specimens of breast cancer patients. The current study suggests that resistin-stimulated ADSCs may interact with breast cancer cells in the tumor microenvironment via CXCL5 secretion, leading to breast cancer cell malignancy.

show abstract

“…1 Advocates for fat grafting to healthy breast tissue remain cautious about potential risks of breast cancer development in these patients. 26,[28][29][30] These proponents emphasize the necessity of continued breast cancer screening and long-term studies to assess any potential increased risk introduced to individuals undergoing these procedures. 1…”

Section: Discussionmentioning

confidence: 99%

“…Lastly, analyses of large cohorts of data suggest that fat grafting was in fact preferred to breast implant therapy for minimizing short-term complication risk 1 . Advocates for fat grafting to healthy breast tissue remain cautious about potential risks of breast cancer development in these patients 26,28–30 . These proponents emphasize the necessity of continued breast cancer screening and long-term studies to assess any potential increased risk introduced to individuals undergoing these procedures 1 …”

Section: Discussionmentioning

confidence: 99%

The Safety of Fat Grafting: An Institutional Retrospective Review

et al. 2022

View full text Add to dashboard Cite

Background Autologous fat grafting is a popular technique for volume replacement in the breast and face. The efficacy, safety, and complication rate of this technique at the division of plastic surgery at the University of Alabama at Birmingham will be described in this review. Methods An institutional review board–approved retrospective review of patients undergoing fat grafting procedures from January 2015 to July 2018 was performed. Records were reviewed for fat graft recipient site, donor site, amount grafted, and complications. Continuous variables were compared using either a t test or one-way analysis of variance test. Categorical data were compared using χ2 test. A P value of 0.05 or less was considered statistically significant for all comparisons. Results A total of 396 patients who underwent fat grafting procedures of the face and body from January 2015 through July 2018 met inclusion criteria. Average amount of fat grafted for all grafts was 124.4 +/− 6.74 grams. Two hundred fifty of the grafts (62.7%) involved the bilateral breasts with an average of 140.6 +/− 93.97 g used, 70 per side. Of the 396 patients, 110 (27.8%) experienced complications. Forty three of the complications (10.9%) were considered to be major, which included hematomas/seromas, fat necrosis, dermatitis/cellulitis, and infection. No statistical differences were seen among recipient site complication rate. Types of minor complications were statistically significant per recipient sites with bilateral breasts more likely to experience asymmetry than the other recipient sites (20% for bilateral breasts vs 16% overall, P < 0.05). Fifty nine of the 110 patients (53.6%) had the complications reported to be resolved. Conclusions Fat grafting is a reliable method for volumization of the breasts and face. Minor complications were not infrequent in this case series; however, no life-threatening complications were observed. Continued work needs to be done to use fat grafting beyond traditional measures.

show abstract

Effect of Breast Cancer and Adjuvant Therapy on Adipose-Derived Stromal Cells: Implications for the Role of ADSCs in Regenerative Strategies for Breast Reconstruction

Cited by 6 publications

References 142 publications

Single-cell RNA sequencing has unveiled the contribution of ADSCs in reprogramming metabolism in triple-negative breast cancer

Single-cell RNA sequencing has unveiled the contribution of ADSCs in reprogramming metabolism in triple-negative breast cancer

ADSCs stimulated by resistin promote breast cancer cell malignancy via CXCL5 in a breast cancer coculture model

The Safety of Fat Grafting: An Institutional Retrospective Review

Contact Info

Product

Resources

About