Effect of Brassica oleracea L. (var. capitata) Hydroalcoholic Extract on Epithelial Layer Healing of Skin in Male Mice

Abstract

Aims: Wound healing is a complex physiological process involves a series of successive stages of hemostasis, inflammation, proliferation, and regeneration. This study aimed to investigate the effect of hydroalcoholic extract of Brassica oleracea L. (var. capitata) leaves in comparison with Phenytoin on epithelial layer healing and the holes in the skin of mice. Materials & Methods: This study was done on 40 male NIH Mice between 25-30gr. Animals were randomly divided into 4 groups (n=10). Negative control group received saline, positive control group administrated with 1% Phenytoin and treated groups received 10% and 20% hydroalcoholic extract twice a day until closing holes. Macroscopic and microscopic analysis was performed on days 1, 4, 7, 12 and 15. Statistical analysis was done by SPSS 16 software and Tukey test. Findings: A significant reduction was observed in average size of holes in 20% hydroalcoholic extract treated group compared to the negative control 10% extract at day 7 (p=0.001). There was a significant reduction in average size of holes in 20% extract group compared to 10% extract and negative control groups at day 12. (p=0.007). The skin holes in 20% extract group were closed earlier than the other groups. Epithelial thickness was increased in 20% extract group from 7th day the much faster than other groups. Conclusion: Brassica oleracea L. (var. capitata) has the same effect of phenytoin in healing of rapid closure of holes and proliferation of epithelial cells.