Effect of bowel transection and fecal passage deprivation on the enteric nervous system in neonatal rats

Govaert, B.; Heurn, Ernst van; Farla, Pascal; Gemert, Wim G. van; Baeten, C. G. M. I.

doi:10.1016/j.jpedsurg.2008.04.031

Cited by 4 publications

(3 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although we did not detect any changes in the ratio of VIP staining neurons, previous studies have described inhibitory and stimulatory effects of proximal and mid bowel resection on VIP abundance in the ENS [43, 44]. Inconsistencies in results could be due to the absence of transected bowel controls, or the effect of location and extent of resection on specific neuroeffectors [43–46]. Interestingly, neuronal responses evoked by intestinal resection were not recapitulated by exogenous GLP-2 infusion.…”

Section: Discussioncontrasting

confidence: 55%

Effects of exogenous glucagon-like peptide-2 and distal bowel resection on intestinal and systemic adaptive responses in rats

et al. 2017

View full text Add to dashboard Cite

ObjectiveTo determine the effects of exogenous glucagon-like peptide-2 (GLP-2), with or without massive distal bowel resection, on adaptation of jejunal mucosa, enteric neurons, gut hormones and tissue reserves in rats.BackgroundGLP-2 is a gut hormone known to be trophic for small bowel mucosa, and to mimic intestinal adaptation in short bowel syndrome (SBS). However, the effects of exogenous GLP-2 and SBS on enteric neurons are unclear.MethodsSprague Dawley rats were randomized to four treatments: Transected Bowel (TB) (n = 8), TB + GLP-2 (2.5 nmol/kg/h, n = 8), SBS (n = 5), or SBS + GLP-2 (2.5 nmol/kg/h, n = 9). SBS groups underwent a 60% jejunoileal resection with cecectomy and jejunocolic anastomosis. All rats were maintained on parenteral nutrition for 7 d. Parameters measured included gut morphometry, qPCR for hexose transporter (SGLT-1, GLUT-2, GLUT-5) and GLP-2 receptor mRNA, whole mount immunohistochemistry for neurons (HuC/D, VIP, nNOS), plasma glucose, gut hormones, and body composition.ResultsResection increased the proportion of nNOS immunopositive myenteric neurons, intestinal muscularis propria thickness and crypt cell proliferation, which were not recapitulated by GLP-2 therapy. Exogenous GLP-2 increased jejunal mucosal surface area without affecting enteric VIP or nNOS neuronal immunopositivity, attenuated resection-induced reductions in jejunal hexose transporter abundance (SGLT-1, GLUT-2), increased plasma amylin and decreased peptide YY concentrations. Exogenous GLP-2 attenuated resection-induced increases in blood glucose and body fat loss.ConclusionsExogenous GLP-2 stimulates jejunal adaptation independent of enteric neuronal VIP or nNOS changes, and has divergent effects on plasma amylin and peptide YY concentrations. The novel ability of exogenous GLP-2 to modulate resection-induced changes in peripheral glucose and lipid reserves may be important in understanding the whole-body response following intestinal resection, and is worthy of further study.

show abstract

Section: Discussioncontrasting

confidence: 55%

Effects of exogenous glucagon-like peptide-2 and distal bowel resection on intestinal and systemic adaptive responses in rats

et al. 2017

View full text Add to dashboard Cite

show abstract

“…While 3 of the 4 patients from which tissue was collected underwent pull‐through without prior surgery, one child had previously had a stoma placed before the pull‐through surgery from which the tissue was collected. It has been demonstrated in neonatal rats 28 that transection of the bowel to form a colostomy can change the morphology of the ENS in the distal colon. Govaert et al, 28 demonstrated that the transection of the bowel caused a change in the composition of neurons and ganglia of the ENS due to a lack of activity in the bowel.…”

Section: Discussionmentioning

confidence: 99%

“…It has been demonstrated in neonatal rats 28 that transection of the bowel to form a colostomy can change the morphology of the ENS in the distal colon. Govaert et al, 28 demonstrated that the transection of the bowel caused a change in the composition of neurons and ganglia of the ENS due to a lack of activity in the bowel. Despite these findings in a rat model, there has been no subsequent evidence for a similar phenomenon in humans.…”

Section: Discussionmentioning

confidence: 99%

The transition zone in Hirschsprung's bowel contains abnormal hybrid ganglia with characteristics of extrinsic nerves

Smith

Chhabra

Shukla

et al. 2023

J Cellular Molecular Medi

View full text Add to dashboard Cite

The aganglionic bowel in short‐segment Hirschsprung's disease is characterized both by the absence of enteric ganglia and the presence of extrinsic thickened nerve bundles (TNBs). The relationship between the TNBs and the loss of enteric ganglia is unknown. Previous studies have described decreasing numbers of ganglia with increasing density of TNBs within the transition zone (TZ) between ganglionic and aganglionic gut, and there is some evidence of spatial contact between them in this region. To determine the cellular interactions involved, we have analysed the expression of perineurial markers of TNBs and enteric ganglionic markers for both neural cells and their ensheathing telocytes across four cranio‐caudal segments consisting of most proximal ganglionic to most distal aganglionic from pull‐through resected colon. We show that in the TZ, enteric ganglia are abnormal, being surrounded by perineurium cells characteristic of TNBs. Furthermore, short processes of ganglionic neurons extend caudally towards the aganglionic region, where telocytes in the TNB are located between the perineurium and nerve fibres into which they project telopodes. Thus, enteric ganglia within the TZ have abnormal structural characteristics, the cellular relationships of which are shared by the TNBs. These findings will help towards elucidation of the cellular mechanisms involved in the aetiology of Hirschsprung's disease.

show abstract

The transition zone in Hirschsprung’s bowel contains abnormal hybrid ganglia with characteristics of extrinsic nerves

Smith

Chhabra

Shukla

et al. 2022

Preprint

View full text Add to dashboard Cite

The aganglionic bowel in short segment Hirschsprung′s disease is characterised both by the absence of enteric ganglia and the presence of extrinsic thickened nerve bundles (TNBs). The relationship between the TNBs and the loss of enteric ganglia is unknown. Previous studies have described decreasing numbers of ganglia with increasing density of TNBs within the transition zone (TZ) between ganglionic and aganglionic gut, and there is some evidence of spatial contact between them in this region. To determine the cellular interactions involved, we have analysed the expression of perineurial markers of TNBs and enteric ganglionic markers for both neural cells and their ensheathing telocytes across four cranio-caudal segments of the TZ. We show that in the TZ, enteric ganglia are abnormal, being surrounded by perineurium cells characteristic of TNBs. Furthermore, short processes of ganglionic neurons extend caudally towards the aganglionic region, where telocytes in the TNB are located between the perineurium and nerve fibres into which they project telopodes. Thus, ENS ganglia within the TZ have abnormal structural characteristics, the cellular relationships of which are shared by the TNBs. These findings will help towards elucidation of the cellular mechanisms involved in the aetiology of Hirschsprung′s disease.

show abstract

Effect of bowel transection and fecal passage deprivation on the enteric nervous system in neonatal rats

Cited by 4 publications

References 10 publications

Effects of exogenous glucagon-like peptide-2 and distal bowel resection on intestinal and systemic adaptive responses in rats

Effects of exogenous glucagon-like peptide-2 and distal bowel resection on intestinal and systemic adaptive responses in rats

The transition zone in Hirschsprung's bowel contains abnormal hybrid ganglia with characteristics of extrinsic nerves

The transition zone in Hirschsprung’s bowel contains abnormal hybrid ganglia with characteristics of extrinsic nerves

Contact Info

Product

Resources

About