Effect of Bovine Lactoferrin on Extracellular Matrix Calcification by Human Osteoblast-Like Cells

Takayama, Yoshiharu; Mizumachi, Koko

doi:10.1271/bbb.70465

Cited by 38 publications

(25 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It promotes the differentiation and bone-forming activity of osteoblasts (Cornish et al 2004). Previously, we have shown that lactoferrin promotes osteogenic differentiation of human osteoblast-like MG63 cells (Takayama and Mizumachi 2008). On the other hand, lactoferrin inhibits the genesis and bone-resorbing activity of osteoclasts (Lorget et al 2002).…”

Section: Discussionmentioning

confidence: 95%

“…Furthermore, lactoferrin is a major component of neutrophil secretory granules, playing important roles in inflammatory-and immune-modulating processes via regulation of lymphocyte activity (Legrand et al 2008). Recent in vivo and in vitro studies have indicated that lactoferrin promotes development of skeletal tissue by up-regulating the growth and differentiation of osteoblasts, as well as inhibiting osteoblast apoptosis (Cornish et al 2004;Takayama and Mizumachi 2008;Blais et al 2009;Guo et al 2009). In addition, lactoferrin has been shown to inhibit the differentiation and bone resorbing activity of osteoclasts (Cornish et al 2004;Lorget et al 2002).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Inhibitory effect of lactoferrin on hypertrophic differentiation of ATDC5 mouse chondroprogenitor cells

Takayama

Mizumachi

2010

Biometals

Self Cite

View full text Add to dashboard Cite

The skeleton is formed by two different mechanisms. In intramembranous ossification, osteoblasts form bone directly, whereas in endochondral ossification, chondrocytes develop a cartilage template, prior to osteoblast-mediated skeletogenesis. Lactoferrin is an iron-binding glycoprotein belonging to the transferrin family. It is known to promote the growth and differentiation of osteoblasts. In this study, we investigated the effects of bovine lactoferrin on the chondrogenic differentiation of ATDC5 chondroprogenitor cells. This mouse embryonic carcinoma-derived clonal cell line provides an in vitro model of chondrogenesis. Lactoferrin treatment of differentiating ATDC5 cells promoted cell proliferation in the initial stage of the differentiation process. However, lactoferrin treatment resulted in inhibition of hypertrophic differentiation, characterized by suppression of alkaline phosphatase activity, aggrecan synthesis and N-cadherin expression. This inhibitory effect was accompanied by sustained Sox9 expression, as well as increased Smad2/3 expression and phosphorylation, suggesting that lactoferrin regulates chondrogenic differentiation by up-regulating the Smad2/3-Sox9 signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Inhibitory effect of lactoferrin on hypertrophic differentiation of ATDC5 mouse chondroprogenitor cells

Takayama

Mizumachi

2010

Biometals

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many bone metabolic diseases develop when there is an imbalance between the formation and resorption of bone, which depend on the interactions between osteoblasts and osteoclasts [12]. Interestingly, LF has been reported to stimulate proliferation and differentiation of osteoblasts [13][14][15] and to inhibit the differentiation of osteoclasts [13,16]. Thus, LF may be a promising therapeutic agent for treatment of bone diseases.…”

Section: Introductionmentioning

confidence: 99%

Effect of lactoferrin on osteogenic differentiation of human adipose stem cells

Ying

Cheng

Wang

et al. 2011

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

Purpose Many in vitro studies of the analysis of the lactoferrin (LF) effect on cells have been reported. However, no study has yet investigated the effect of LF on osteogenic differentiation of human adipose-derived stem cells (hADSCs). The aim of this study was to evaluate the effect of LF on osteogenic differentiation of human adipose stem cells.Methods The hADSCs were cultured in an osteogenic medium with 0, 10, 50 and 100 μg/ml LF, respectively. hADSC proliferation was analysed by Cell Counting Kit-8 (CCK-8) assay, and cell osteogenic differentiation was evaluated by alkaline phosphatase (ALP) activity assay, von Kossa staining and real-time polymerase chain reaction (RT-PCR). Results Cell proliferation was significantly increased by LF in a dose-dependent manner from days 4 to 14. Cells cultured with 100 μg/ml LF presented a higher activity compared with the control. The deposition of calcium was increased after the addition of LF. The mRNA expression of type I collagen (COL-I), ALP, osteocalcin (OCN) and RUNX2 increased markedly as a result of LF treatment. Conclusions We have shown for the first time that LF could promote the proliferation and osteogenic differentiation of hADSCs, which could be a promising approach for enhancing osteogenic capacity of cell-based construction in bone tissue engineering.

show abstract

“…Two types of functional bone cells, osteoblasts and osteoclasts, regulate bone metabolism: osteoblasts are responsible for bone formation, whereas osteoclasts reabsorb bone (Nijweide et al 1986). Interestingly, bLF has been reported to stimulate proliferation and differentiation of osteoblasts Blais et al 2009;Takayama and Mizumachi 2009;Takayama and Mizumachi 2008) and to inhibit the differentiation of osteoclasts Blais et al 2009;Lorget et al 2002). Thus, bLF may be a promising therapeutic agent for treatment of osteoporosis.…”

Section: Introductionmentioning

confidence: 99%

Bovine milk lactoferrin induces synthesis of the angiogenic factors VEGF and FGF2 in osteoblasts via the p44/p42 MAP kinase pathway

et al. 2011

View full text Add to dashboard Cite

Lactoferrin (LF) belongs to the transferrin family and is present in several physiological fluids, including milk and colostrum. LF has recently been identified as an anabolic factor for bone. Here we investigated whether bovine LF (bLF) induces synthesis of angiogenic factors by osteoblasts. If so, we examined the underlying mechanism. We found that bLF purified from milk increased the mRNA expression of vascular endothelial growth factor (VEGF) and fibroblast growth factor-2 (FGF2) in murine osteoblast-like MC3T3-E1 cells and primary murine osteoblasts in a time- and dose-dependent manner. Furthermore, bLF increased VEGF and FGF2 protein levels in MC3T3-E1 cells. In addition, treatment of MC3T3-E1 cells with bLF rapidly induced phosphorylation of p44/p42 mitogen-activated protein (MAP) kinase. The bLF-mediated increases in VEGF and FGF2 mRNA and protein were inhibited by U0126, a specific inhibitor of the upstream kinase that activates p44/p42 MAP kinase (MEK). Taken together, our results strongly suggest that bLF induces VEGF and FGF2 synthesis in a p44/p42 MAP kinase-dependent manner in MC3T3-E1 cells.

show abstract

Effect of Bovine Lactoferrin on Extracellular Matrix Calcification by Human Osteoblast-Like Cells

Cited by 38 publications

References 15 publications

Inhibitory effect of lactoferrin on hypertrophic differentiation of ATDC5 mouse chondroprogenitor cells

Inhibitory effect of lactoferrin on hypertrophic differentiation of ATDC5 mouse chondroprogenitor cells

Effect of lactoferrin on osteogenic differentiation of human adipose stem cells

Bovine milk lactoferrin induces synthesis of the angiogenic factors VEGF and FGF2 in osteoblasts via the p44/p42 MAP kinase pathway

Contact Info

Product

Resources

About