Effect of bound water content on cell adhesion strength to water-insoluble polymers

Nishida, Kei; Anada, Takahisa; Kobayashi, Shū; Ueda, Tomoya; Tanaka, Masaru

doi:10.1016/j.actbio.2021.07.058

Cited by 28 publications

(31 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are in line with previous works where water content of κ-c hydrogels was reported as 99% [47]. It is important to note that there has been reported a strong correlation between materials hydration and cell adhesion [50].…”

Section: Properties Of Casted κ-Carrageenan (κ-C) Hydrogelssupporting

confidence: 93%

3D Bioprinting of Novel κ-Carrageenan Bioinks: An Algae-Derived Polysaccharide

et al. 2022

View full text Add to dashboard Cite

Novel green materials not sourced from animals and with low environmental impact are becoming increasingly appealing for biomedical and cellular agriculture applications. Marine biomaterials are a rich source of structurally diverse compounds with various biological activities. Kappa-carrageenan (κ-c) is a potential candidate for tissue engineering applications due to its gelation properties, mechanical strength, and similar structural composition of glycosaminoglycans (GAGs), possessing several advantages when compared to other algae-based materials typically used in bioprinting such as alginate. For those reasons, this material was selected as the main polysaccharide component of the bioinks developed herein. In this work, pristine κ-carrageenan bioinks were successfully formulated for the first time and used to fabricate 3D scaffolds by bioprinting. Ink formulation and printing parameters were optimized, allowing for the manufacturing of complex 3D structures. Mechanical compression tests and dry weight determination revealed young’s modulus between 24.26 and 99.90 kPa and water contents above 97%. Biocompatibility assays, using a mouse fibroblast cell line, showed high cell viability and attachment. The bioprinted cells were spread throughout the scaffolds with cells exhibiting a typical fibroblast-like morphology similar to controls. The 3D bio-/printed structures remained stable under cell culture conditions for up to 11 days, preserving high cell viability values. Overall, we established a strategy to manufacture 3D bio-/printed scaffolds through the formulation of novel bioinks with potential applications in tissue engineering and cellular agriculture.

show abstract

Section: Properties Of Casted κ-Carrageenan (κ-C) Hydrogelssupporting

confidence: 93%

3D Bioprinting of Novel κ-Carrageenan Bioinks: An Algae-Derived Polysaccharide

et al. 2022

View full text Add to dashboard Cite

show abstract

“…11 Adsorbed water and bound water are not detrimental to the stability of the drug. 12 13 Simply knowing the total water content may not be the most reliable method for understanding the effects of water on drug stability. Unlike adsorbed and bound water, free water has the same energy and properties as pure water, which is available for chemical reactions.…”

Section: Resultsmentioning

confidence: 99%

Effect of Oxygen and Water on the Stability of Imipenem and Cilastatin Sodium for Injection

Meng

Liu

Chen

et al. 2022

Pharmaceutical Fronts

View full text Add to dashboard Cite

The study aimed to investigate the factors affecting the stability of imipenem and cilastatin sodium for injection (IMI/CIL) to improve the quality and stability in IMI/CIL preparation. In this study, the effects of headspace oxygen (HO), water content, particle shape, and particle size on the stability of IMI/CIL were investigated. IMI/CIL was purged with air, premixed oxygen/nitrogen gas (5%/95%), or high-purity nitrogen (99.999%) at 20, 5, or 2% oxygen levels to prepare IMI/CIL with different HO levels. IMI/CIL was stored at 30, 45, and 75% relative humidity for 30 days to prepare IMI/CIL with different water contents. High-performance liquid chromatography method was used for analysis. The results showed that oxygen, water, particle shape, and particle size had significant effects on the stability of IMI/CIL, and free water content is a better predictor of the safety and stability of imipenem and cilastatin sodium than the total water content. The optimization scheme of the above parameters is proposed, which significantly improves the stability of IMI/CIL. This study led to a better understanding of the degradation mechanism of imipenem and cilastatin sodium, and could provide a reference for the selection and control of IMI/CIL process conditions. This study would contribute to the development of IMI/CIL with improved stability.

show abstract

“…The force-distance curves between the cells and the substrates were measured using an AFM (CellHesion JPK; Bruker, Billerica, MA, USA) equipped with a tipless cantilever (set point: 2 nN, approaching rate: 5.0 µm/s, holding time: 120 s, retraction rate: 15 µm/s). The value of set point for the measurement of HUVEC adhesion strength was used from our previous report, where the relationship between the set points and the cell adhesion strength of HeLa cells were evaluated [37]. In this investigation, the maximum force for cell detachment from the substrate is denoted as adhesion force, which is indicated at the lowest point of the retraction curve.…”

Section: Huvec-pmea and Huvec-huvec Interaction Determined By Scfsmentioning

confidence: 99%

Poly(2-Methoxyethyl Acrylate) (PMEA)-Coated Anti-Platelet Adhesive Surfaces to Mimic Native Blood Vessels through HUVECs Attachment, Migration, and Monolayer Formation

et al. 2022

Self Cite

View full text Add to dashboard Cite

Confluent monolayers of human umbilical vein endothelial cells (HUVECs) on a poly(2-methoxyethyl acrylate) (PMEA) antithrombogenic surface play a major role in mimicking the inner surface of native blood vessels. In this study, we extensively investigated the behavior of cell–polymer and cell–cell interactions by measuring adhesion strength using single-cell force spectroscopy. In addition, the attachment and migration of HUVECs on PMEA-analogous substrates were detected, and the migration rate was estimated. Moreover, the bilateral migration of HUVECs between two adjacent surfaces was observed. Furthermore, the outer surface of HUVEC was examined using frequency-modulation atomic force microscopy (FM-AFM). Hydration was found to be an indication of a healthy glycocalyx layer. The results were compared with the hydration states of individual PMEA-analogous polymers to understand the adhesion mechanism between the cells and substrates in the interface region. HUVECs could attach and spread on the PMEA surface with stronger adhesion strength than self-adhesion strength, and migration occurred over the surface of analogue polymers. We confirmed that platelets could not adhere to HUVEC monolayers cultured on the PMEA surface. FM-AFM images revealed a hydration layer on the HUVEC surfaces, indicating the presence of components of the glycocalyx layer in the presence of intermediate water. Our findings show that PMEA can mimic original blood vessels through an antithrombogenic HUVEC monolayer and is thus suitable for the construction of artificial small-diameter blood vessels.

show abstract

Effect of bound water content on cell adhesion strength to water-insoluble polymers

Cited by 28 publications

References 66 publications

3D Bioprinting of Novel κ-Carrageenan Bioinks: An Algae-Derived Polysaccharide

3D Bioprinting of Novel κ-Carrageenan Bioinks: An Algae-Derived Polysaccharide

Effect of Oxygen and Water on the Stability of Imipenem and Cilastatin Sodium for Injection

Poly(2-Methoxyethyl Acrylate) (PMEA)-Coated Anti-Platelet Adhesive Surfaces to Mimic Native Blood Vessels through HUVECs Attachment, Migration, and Monolayer Formation

Contact Info

Product

Resources

About