Effect of Boric Acid Solution on Cartilage Metabolism

Benderdour, Mohamed; Heß, K.; Gadet, Michel Dzondo; Dousset, B.; Nabet, P; Belleville, F.

doi:10.1006/bbrc.1997.6621

Cited by 45 publications

(30 citation statements)

References 18 publications

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“…Similarly, the formation of glucose-boron complexes with in vivo action of boron diminished energetic substrates in cartilage cells (Benderdour et al 1997). Here the data consistently indicated a causal association between exposure to high dose (4.5 mmol B/kg b.wt) of BA and SS mitochondria.…”

Section: Discussionsupporting

confidence: 70%

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Acute toxicity of boric acid on energy metabolism of the breast muscle in broiler chickens

Geyikoğglu

Türkez

2007

Biologia

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Short-and long-term oral exposures to boric acid (BA) in laboratory animals and birds caused toxic effects. However, the toxicity data on adult poultry skeletal muscles of BA was not documented with metabolic studies. Livability, weight gain, and feed conversion might be adversely affected in broilers as a result of changes in energy metabolism. Therefore, this study was conducted to investigate the influences of acute BA doses on energy metabolism of chick pectoral muscle (PM). Chicks were fed by giving the aqueous solutions supplemented with BA (0, 0.27, 0.54, 1.08, 1.35, 2.25, 3.375 and 4.5 mmol B/kg b.wt.). Breast muscle samples were taken at 24, 48 and 72 h and analyzed histochemically, ultrastructurally and biochemically. Data collected in these analyses indicated that consumption of diets containing up to 2.25 mmol B/kg at 24, 48 and 72h was not detrimental to broiler PM. However, 3.375 mmol B/kg b.wt. (at 24 h) and 4.5 mmol B/kg b.wt.(at 24 and 48 h) caused decreased metabolite concentrations (glucose, glycogen, lactate and ATP) in muscle fibers (Type IIB). Subsarcolemmal (SS) mitochondria and intermyofibrillar (IM) mitochondrial damage (cristae dissolution) were also observed by toxic effect of BA (4.5 mmol B/kg b.wt.). These observations proved that BA at the high doses (3.375 and 4.5 mmol B/kg b.wt.) causes to altered energy metabolism in Type IIB as dependent on time. Based on these results we think that energy protection in muscle against BA toxicity will be the most important study subject. Thus, high BA doses will not have detrimental effects on broiler performance.

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Section: Discussionsupporting

confidence: 70%

“…A little is known concerning the role of BA on cellular level (Benderdour et al 1997). It is not known whether the active toxicant is BA itself or a molecule(s) combined with BA in target tissues (Moore 1997).…”

Section: Discussionmentioning

confidence: 99%

Acute toxicity of boric acid on energy metabolism of the breast muscle in broiler chickens

Geyikoğglu

Türkez

2007

Biologia

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“…They were carried out according to the method described by Benderdour et al (1997) with of each supernatant and anti-hsp 70 monoclonal antibody (Sigman H5147), diluted 1/5,000 in PBS-Tween 20 or rabbit-polyclonal anti-human TNFα antibody diluted 1/250 (Genzyme IP 130) or polyclonal antibody anti-human VEGF (calbiochem Ref. 676472) diluted 1/250.…”

Section: Sds-page Electrophoresis and Immunoblottingmentioning

confidence: 99%

“…676472) diluted 1/250. The membranes were washed three times in PBS-Tween and incubated with second antibody diluted 1/8,000 in PBS-Tween 20 (rabbit monoclonal antimouse IgG conjugated to peroxidase, Sigma A 9044) or with second antibody diluted 1/6,000 in PBS-Tween 20 (anti-rabbit IgG conjugated to peroxidase, Sigma A 0545) (Benderdour et al, 1997).…”

Section: Sds-page Electrophoresis and Immunoblottingmentioning

confidence: 99%

“…The migration of endothelial cells in the wound, their proliferation and the basement membrane degradation are involved in this process (Madri et Bell, 1992). The effect of boron is perhaps due to the growth factors release (Benderdour et al, 1997) such as which stimulates protease activity particularly collagenase activity (Bettinger et al, 1994). The VEGF (vascular endothelium growth factor) which is also a potent-up regulator of angiogenesis, promotes the endothelial cell proliferation and migration, induces macrophage migration and modifies extracellular matrix composition leading to the angiogenesis (Frelin et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

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