Effect of bone marrow and adipose tissue-derived mesenchymal stem cells on the natural course of corneal scarring after penetrating injury

Demirayak, Bengi; Yüksel, Nurşen; Çelik, Onur Sinan; Subaşı, Cansu; Duruksu, Gökhan; Ünal, Zehra Seda; Yıldız, Demir Kürşat; Karaöz, Erdal

doi:10.1016/j.exer.2016.08.011

Cited by 56 publications

(40 citation statements)

References 32 publications

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“…BMMSC are well defined as plastic-adherent cells positive for CD73, CD90, CD105, while negative for CD11b, CD14, CD19, CD34, CD45, CD79a or HLA-DR surface molecules and can be differentiated into osteoblasts, adipocytes and chondroblasts in vitro [8]. BMMSC have also been shown to differentiate into corneal epithelial like cells [9,10] and keratocytes [11,12]. In fact, BMMSC transplantation has been shown to promote cornea wound healing after alkali burn [9,10,[13][14][15][16] and facilitate the regeneration of the corneal stroma after penetrating injury [12].…”

Section: Ivyspringmentioning

confidence: 99%

Differential Gene Expression between Limbal Niche Progenitors and Bone Marrow Derived Mesenchymal Stem Cells

Wang¹,

Shen²,

Xu³

et al. 2020

Int. J. Med. Sci.

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Purpose: To compare the difference in gene expression between human limbal niche cells (LNC) and bone marrow derived mesenchymal stem cells (BMMSC). Methods: LNC were isolated by collagenase and expanded in modified embryonic stem cell medium (MESCM) on a Matrigel coated plastic plate. Cell diameters were measured with Image J software. Relative gene expression levels between LNC and BMMSC were compared using Affymetrix Human Primer View Gene Expression Array. A subset of differentially expressed genes was verified by RT-qPCR. The protein level of LAMA1 and COL4A1 was confirmed by Western blot and immunostaining. Results: The average diameter of LNC was 10.2±2.4 μm, which was significantly smaller than that of BMMSC (14 ±3.4 μm) (p<0.0001). Expression of 20,432 genes was examined by Gene Expression Array, among which expression of 349 genes in LNC was 10-fold or higher than that of BMMSC and expression of 8 genes in LNC was 100-fold or higher than that of BMMSC, while expression of 3 genes in BMMSC was 100-fold higher than that of LNC. GO analysis and pathway analysis showed that the differentially expressed genes were mainly enriched in the extracellular matrix receptor interaction pathway and Wnt signaling pathway. In addition, RT-qPCR results demonstrated that the expression of CD73, CD90, CD105, PDGFRβ, Vimentin, SCF, KIT (CD117), COL14A1, LAMA2, THBS2, FZD1, BMP2 and CXCL12 genes in LNC were at least 2 folds higher than BMMSC. The protein level of LAMA1 was higher but the protein level of COL4A1 was lower in LNC than that in BMMSC. Conclusion: LNC exhibit differential gene expression from BMMSC in the extracellular matrix (ECM) receptor interaction pathway and Wnt signaling pathway, suggesting that LNC have their unique signaling pathways to support limbal stem cell niches.

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Section: Ivyspringmentioning

confidence: 99%

Differential Gene Expression between Limbal Niche Progenitors and Bone Marrow Derived Mesenchymal Stem Cells

Wang¹,

Shen²,

Xu³

et al. 2020

Int. J. Med. Sci.

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“…Moreover, a broad area of research focusses on mesenchymal stem cells (MSCs) derived from non-ocular tissues to promote corneal and skin regeneration. MSCs derived from bone marrow, adipose tissue, umbilical cord or dental pulp [211,212] were demonstrated to acquire both epithelial cell-like [213,214] and keratocyte-like characteristics [215,216,217] (see [195] for a systematic review). The therapeutic use of bone-marrow or adipose tissue-derived MSCs, by local injection or topical application in animal models of chemical burns, was shown to improve corneal healing and reduce corneal inflammation and neovascularization [218,219,220,221].…”

Section: Concluding Remarks and Future Directionsmentioning

confidence: 99%

Wound-Healing Studies in Cornea and Skin: Parallels, Differences and Opportunities

Bukowiecki

Hos

Cursiefen

et al. 2017

IJMS

142

136

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The cornea and the skin are both organs that provide the outer barrier of the body. Both tissues have developed intrinsic mechanisms that protect the organism from a wide range of external threats, but at the same time also enable rapid restoration of tissue integrity and organ-specific function. The easy accessibility makes the skin an attractive model system to study tissue damage and repair. Findings from skin research have contributed to unravelling novel fundamental principles in regenerative biology and the repair of other epithelial-mesenchymal tissues, such as the cornea. Following barrier disruption, the influx of inflammatory cells, myofibroblast differentiation, extracellular matrix synthesis and scar formation present parallel repair mechanisms in cornea and skin wound healing. Yet, capillary sprouting, while pivotal in proper skin wound healing, is a process that is rather associated with pathological repair of the cornea. Understanding the parallels and differences of the cellular and molecular networks that coordinate the wound healing response in skin and cornea are likely of mutual importance for both organs with regard to the development of regenerative therapies and understanding of the disease pathologies that affect epithelial-mesenchymal interactions. Here, we review the principal events in corneal wound healing and the mechanisms to restore corneal transparency and barrier function. We also refer to skin repair mechanisms and their potential implications for regenerative processes in the cornea.

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“…Such cells also contributed to increased strength of adhesion of flaps created in the laser‐assisted in situ keratomileusis procedure in organ‐cultured corneas, which may be clinically important for flap integrity . Additional to these endogenous cells, various MSCs in vivo significantly reduced stromal neovascularization in alkaline burn models and diminished corneal opacity and inflammation upon penetrating injury .…”

Section: Stem Cells For Stromal Wound Healingmentioning

confidence: 99%

Concise Review: Stem Cells for Corneal Wound Healing

et al. 2017

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Corneal wound healing is a complex process that occurs in response to various injuries and commonly used refractive surgery. It is a significant clinical problem, which may lead to serious complications due to either incomplete (epithelial) or excessive (stromal) healing. Epithelial stem cells clearly play a role in this process, whereas the contribution of stromal and endothelial progenitors is less well studied. The available evidence on stem cell participation in corneal wound healing is reviewed, together with the data on the use of corneal and non‐corneal stem cells to facilitate this process in diseased or postsurgical conditions. Important aspects of corneal stem cell generation from alternative cell sources, including pluripotent stem cells, for possible transplantation upon corneal injuries or in disease conditions are also presented. Stem Cells 2017;35:2105–2114

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Effect of bone marrow and adipose tissue-derived mesenchymal stem cells on the natural course of corneal scarring after penetrating injury

Cited by 56 publications

References 32 publications

Differential Gene Expression between Limbal Niche Progenitors and Bone Marrow Derived Mesenchymal Stem Cells

Differential Gene Expression between Limbal Niche Progenitors and Bone Marrow Derived Mesenchymal Stem Cells

Wound-Healing Studies in Cornea and Skin: Parallels, Differences and Opportunities

Concise Review: Stem Cells for Corneal Wound Healing

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