Effect of Bombesin and Its Mammalian Counterpart, GRP, on Exocrine Pancreas in the Rat

Varga, Gábor; Papp, M.; Dobronyi, I.; Scarpignato, Carmelo

doi:10.1159/000199795

Cited by 15 publications

(5 citation statements)

References 26 publications

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“…As previously described (Varga et al, 1985;1988a) administration of either caerulein or bombesin increased the weight of the pancreas and the total pancreatic protein and DNA, trypsin and amylase contents (Figures 5-7). Lorglumide, administered alone did not significantly affect pancreatic size and composition; it did, however, significantly reduce the caerulein-induced increase in pancreatic weight, protein and enzyme content (Figures 5-7).…”

Section: In Vivo Secretory Studiessupporting

confidence: 66%

Effect of a new potent CCK antagonist, lorglumide, on caerulein‐ and bombesin‐induced pancreatic secretion and growth in the rat

Scarpignato

Varga

Dobronyi

et al. 1989

British J Pharmacology

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1 The effect of lorglumide, a new potent cholecystokinin (CCK) antagonist, on pancreatic secretion and growth induced by caerulein and bombesin was studied in the rat. 2 Pancreatic exocrine secretion was studied both in vitro (isolated and perfused pancreatic segments) and in vivo (anaesthetized animals with cannulation of the common bile duct) whereas the trophic effect was investiged after short-term (5 days) administration of the peptides and/or lorglumide. 3 Both caerulein and bombesin stimulated amylase release from in vitro pancreatic segments in a concentration-dependent manner. Although the efficacy of both peptides was virtually identical, the potency of caerulein was higher than that of bombesin. Lorglumide displaced the concentrationresponse curves to caerulein to the right without affecting the maximum response, suggesting a competitive antagonism. The Schild plot analysis of data gave a straight line with a slope not significantly different from unity. The calculated pA2 for lorglumide was 7.31 + 0.45. The antagonist, however, was completely ineffective when tested against bombesin-induced amylase release. 4 In vivo experiments confirmed results from in vitro studies since lorglumide (5 and I0mgkg 1) significantly reduced pancreatic exocrine secretion induced by caerulein without affecting the response to bombesin. 5 Administration of either peptide increased the weight of the pancreas, the total pancreatic protein and DNA, trypsin and amylase content. Lorglumide (10mgkg-1), administered together with caerulein, reduced the peptide-induced increase in pancreatic weight, protein and enzyme content. On the contrary, when lorglumide was given together with bombesin, all the parameters that were examined were not altered by concomitant administration of the antagonist. 6 These results have demonstrated the ability of lorglumide to antagonize the effects on the pancreas of a CCK-analogue, caerulein, and its inability to affect bombesin-induced pancreatic secretion and growth, suggesting that lorglumide is a potent and selective antagonist of CCK-receptors in the pancreas.

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Section: In Vivo Secretory Studiessupporting

confidence: 66%

Effect of a new potent CCK antagonist, lorglumide, on caerulein‐ and bombesin‐induced pancreatic secretion and growth in the rat

Scarpignato

Varga

Dobronyi

et al. 1989

British J Pharmacology

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“…Another hormonal peptide, bombesin iso lated from the amphibian skin and identified in the gut [9,10], was demonstrated to re lease gastrin and CCK [11,12] and to di rectly stimulate pancreatic secretion and growth [11][12][13][14], Despite numerous studies on the effects of exogenous peptides on pan creatic secretion and growth, the relative contribution of these peptides to the physio logical control of pancreatic functions has not been established.…”

mentioning

confidence: 99%

Antagonism of Receptors for Bombesin, Gastrin and Cholecystokinin in Pancreatic Secretion and Growth

et al. 1991

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The effects of bombesin, gastrin and cholecystokinin (CCK) on amylase secretion from the isolated rat pancreatic acini and on DNA synthesis (as biochemical indicator of trophic action) in the pancreas have been examined in 48-hour fasted and 16-hour refed rats with and without administration of specific receptor antagonists for bombesin, gastrin and CCK. Studies on the isolated rat acini revealed that bombesin, gastrin and CCK-8 all showed the same efficacy in their ability to stimulate amylase release. RC-3095, bombesin pseudopeptide antagonizing bombesin receptors, was effective only in suppressing the amylase response to bombesin but not to gastrin or CCK. Benzodiazepine receptor antagonists for gastrin (L-365,260) and for CCK (L-364,718) showed higher efficacy in the inhibition of amylase release induced by pentagastrin and CCK, respectively, but failed to affect that induced by bombesin. These peptides administered 3 times daily for 48 h in fasted rats increased the rate of DNA synthesis as measured by the incorporation of [³H]thymidine into DNA. The blockade of bombesin receptors abolished the DNA synthesis induced only by bombesin but not by gastrin or CCK. The blockade of gastrin receptors by L-365,260 suppressed the DNA synthesis induced by gastrin while the antagonism of CCK receptors by L-364,718 was effective only against CCK. Refeeding of 48-hour fasting rats strongly enhanced DNA synthesis which was significantly reduced by blocking only the CCK receptors (with L-364,718), but not the bombesin (with RC-3095) or gastrin receptors (with L-365,260). This study indicates that the secretory stimulation of pancreatic acini and DNA synthesis can be achieved by exogenous bombesin, gastrin and CCK acting via separate receptors, but that only CCK plays the major role in the postprandial stimulation of the growth of pancreatic tissue.

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“…This was confirmed by the finding of specific bombesin receptors on isolated pancreatic acini and pancreatic AR42J cells (23,30). In vivo studies also reported a trophic effect on the exocrine pancreas although not as big as the response to caerulein (48).…”

Section: Bombesin and The Pancreassupporting

confidence: 52%

Bombesin

The Pancreapedia: Exocrine Pancreas Knowledge Base

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Effect of Bombesin and Its Mammalian Counterpart, GRP, on Exocrine Pancreas in the Rat

Cited by 15 publications

References 26 publications

Effect of a new potent CCK antagonist, lorglumide, on caerulein‐ and bombesin‐induced pancreatic secretion and growth in the rat

Effect of a new potent CCK antagonist, lorglumide, on caerulein‐ and bombesin‐induced pancreatic secretion and growth in the rat

Antagonism of Receptors for Bombesin, Gastrin and Cholecystokinin in Pancreatic Secretion and Growth

Bombesin

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