“…Inclusion Criteria POAG was diagnosed [1,7,[23][24] if the patient met the following criteria: 1) open anterior chamber angle (Shaffer's grade 3 or 4 in all quadrants); 2) raised IOP (≥22 mm Hg), this is the true IOP after accounting for central corneal thickness (via pachymetry); 3) a vertical cupdisc ratio of ≥0.6 or asymmetry of disc cupping equal to or greater than 0.2 with or without the following disc changes: a rim notching or thinning of the rim, disc haemorrhage or violation of the ISNT (inferior, superior, nasal, temporal) rule, seen on dilated fundoscopy using +78 D in one or both eyes; 4) characteristic glaucomatous visual field defect (such as nasal step, paracentral scotoma, temporal wedge defect, seidel scotoma, arcuate scotoma, double arcuate scotoma using automated Perimetry using Optos Automated Perimeter Model AP 200) may or may not be present. Non-glaucomatous patient was one who in each eye met the following criteria [25][26] : 1) vertical cup-disc ratio of <0.6, no disc asymmetry of ˃0.2, no rim notching or thinning of the rim, no disc haemorrhage or violation of the ISNT rule, and no disc pathology, with pink rim seen on dilated fundoscopy using +78 D; 2) true IOP of <22 mm Hg after pachymetry. Exclusion Criteria Participants were ˂30y, did not give consent to participate in the study, diagnosed with POAG before this study, with other types of glaucoma, previous ocular surgeries or anterior segment pathologies such as acute or chronic uveitis, or any corneal abnormality that would prevent reliable IOP measurement such as corneal opacity, diabetic or hypertensive or blood pressure ˃130/80 mm Hg, subjects on medications known to influence IOP.…”