Effect of BMPRIB gene silencing by siRNA on apoptosis and steroidogenesis of porcine granulosa cells

Abstract

ABSTRACT. Bone morphogenetic proteins (BMPs) are the key factors in maintaining cell growth and differentiation in ovaries. BMPs initiate signaling by assembling BMP receptors and activating Smads, which in turn alter the expression of target genes. However, little is known about the effect of the deletion of the Bone morphogenetic protein receptor type IB (BMPRIB) on porcine granulosa cell (GCs). The objective of this study was to determine the effects of BMPRIB gene silencing, by small interfering RNA (siRNA), on the apoptosis and steroidogenesis of porcine GCs, and the expression of cell cycle-related and apoptosis-related genes. Results indicate that the BMPRIB siRNA caused specific inhibition of BMPRIB mRNA expression after transfection. Knockdown of the BMPRIB gene significantly inhibited porcine GCs proliferation and estradiol production, while inducing apoptosis of porcine GCs. Additionally, the declined expression of the BMPRIB gene changed the expressions of CylinD2, Cdk2, Bcl-2, and Cyp19a1. These

Modulation of granulosa cell function via CRISPR-Cas fuelled editing of BMPR-IB gene in goats (Capra hircus)

“…Our result was consistent with the findings of Silva et al 36 , reported the expression of BMPRs in granulosa cells of antral follicles in goat. Significantly (p < 0.05) higher abundance of BMPR-IB mRNA in granulosa cells obtained from large antral follicles was found in our study is in accordance to earlier reports in goat, pig and buffalo [37][38][39] . The expression of BMPRs in the follicle suggests the potential role of BMP system in affecting the steroidogenesis and functional differentiation of granulosa cells.…”

A bone morphogenetic protein ligand and receptors in mud crab: A potential role in the ovarian development

“…BMPRIB in mouse was demonstrated to involve in estrus cyclicity, estradiol biosynthesis, and cumulus cell expansion in vivo (Soyun et al, 2001). Similar results are reported in the study of pig and ewe (Mulsant et al, 2001;Zhao et al, 2014). As a consequence, BMPRIB of S. paramamosain might regulate vitellogenesis in the ovary.…”

“…In addition, Sp-BMPRIB and Sp-BMPRII had an abundant expression in ovary as well as Sp-BMP7. The higher expression of some BMP ligands and receptors in ovary has been reported in many mammals, such as goat (Lima et al, 2012), hamster (Lima et al, 2012) and swine (Zhao et al, 2014). Thus, the results of tissues distribution, especially the abundant expression of BMP system in ovarian development attracted our attention.…”

“…6). It is controversial with BMPRIB in pig (Zhao et al, 2014) and Sj-BMP parasitic flatworm , which were knocked down after several days. This difference is possibly caused by the species diversity and the experimental method as well.…”

“…According to previous reports, BMPs were involved in follicle growth and ovarian development in vertebrates. It is well-known that BMPs originally bind to two BMP receptors, which in turn activate SMADs and target genes (Zhao et al, 2014). Massive researches have reported that TGF-b signaling was transduced from extra-cellular to cell nucleus via SMAD proteins (Wrana et al, 1992;Heldin et al, 1997;Miyazono, 2000).…”