Effect of Black Pepper (Piper nigrum) Extract on Caffeine-Induced Sleep Disruption and Excitation in Mice

Abstract

Sleep is one of the most essential factors required to maintain good health. However, the global prevalence of insomnia is increasing, and caffeine intake is a major trigger. The objective of this study was to investigate the inhibitory effect of black pepper, Piper nigrum extract (PE), on caffeine-induced sleep disruption and excitation in mice. Caffeine significantly decreased sleep duration in the pentobarbital-induced sleep test. It also resulted in a significant increase in sleep onset and a decrease in non-rapid eye movement sleep. Moreover, in an open-field test, caffeine-treated mice exhibited a significantly increased time in the center zone and total distance traveled. However, the co-administration of caffeine and PE did not result in similar arousal activities. Thus, our results suggest that PE can be used as a potential therapeutic agent to treat sleep problems and excitatory status associated with caffeine intake.

Effects of Chronic Administration of Green Tea Ethanol Extract on Sleep Architecture in Mice: A Comparative Study with a Representative Stimulant Caffeine

“…The delta activity is used as a sleep parameter to evaluate sleep intensity in NREMS [ 43 , 44 ]. This study confirmed that caffeine did not change the delta activity [ 11 ] and that caffeine, as well as GT, induces arousal-inducing effects without a change in the delta activity. To evaluate whether GT presents withdrawal symptoms or adverse effects using EEG and EMG, recordings in withdrawal days were analyzed in the mice.…”

“…The administration of caffeine significantly decreased the number of bouts of wakefulness ranging from 10 to 30 s during the 21 days ( Figure 6 b). However, a previous study reported that a significant decrease was not induced in the number of bouts of wakefulness ranging from 10 to 30 s [ 11 ]. Because we investigated the sleep-promoting effects of extract using the caffeine-induced sleep disruption model, our study was performed to evaluate the arousal-inducing effect of GT.…”

“…Moreover, consumption of caffeine can lead to improved wakefulness and decreased fatigue [ 8 ]. Various studies related to the arousal effects of caffeine in humans and animals have reported prolonged sleep latency and a state of wakefulness [ 9 , 10 , 11 , 12 ]. A recent study reported that coffee is the main source of caffeine, accounting for approximately 80% of total intake of caffeine in adults [ 13 ].…”

Chrysanthemum morifolium and Its Bioactive Substance Enhanced the Sleep Quality in Rodent Models via Cl− Channel Activation

“…Sleep was evaluated based on the prolongation of the pentobarbital-induced sleeping time by the treatments. Sleep evaluation was performed between 10:00 and 16:00 as previously described [ 22 , 23 , 24 ] with slight modification. Two separate different doses of pentobarbital were used.…”

Characterization and Isolation of Piperamides from Piper nigrum Cultivated in Costa Rica

“…Reviews on the use of P. nigrum highlighted the in vivo neuroprotective and hypoglycemic effects presenting a potential for further research, especially to investigate its capacity to inhibit acetylcholinesterase and butyrylcholinesterase, two enzymes critically implicated in Alzheimer's and Parkinson's disorders, but also α-amylase, α-glucosidase, and lipase enzymes [6] as well as other neuroprotective properties reducing caffeineinduced effects on mice hyperlocomotion [7].…”