Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms

Dasso, Maximiliano; Pagotto, Romina; Pignataro, Omar Pedro; Díez, Roberto A.; Sales, Marı́a Elena

doi:10.1016/j.bbagen.2011.08.009

Cited by 6 publications

(3 citation statements)

References 28 publications

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“…Asthma therapies, such as β 2 -adrenoceptor agonists or theophylline, induce bronchodilation by increasing the production of cAMP or reducing the degradation of cyclic nucleotides (cAMP and cGMP). As TAS2R agonists have been suggested to exert their effects via modulation of cyclic nucleotides [139,140], possible interactions between TAS2R agonists and cyclic nucleotides were evaluated in the airway smooth muscle.…”

Section: Cyclic Nucleotides (Camp/cgmp)mentioning

confidence: 99%

Bitter taste receptor agonists mediate relaxation of human and rodent vascular smooth muscle

Manson

Säfholm

Al‐Ameri

et al. 2014

European Journal of Pharmacology

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Section: Cyclic Nucleotides (Camp/cgmp)mentioning

confidence: 99%

Bitter taste receptor agonists mediate relaxation of human and rodent vascular smooth muscle

Manson

Säfholm

Al‐Ameri

et al. 2014

European Journal of Pharmacology

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“…The α-amylase activity in saliva was determined using an enzymatic hydrolysis assay ( 35 , 36 ) with three technical replicates for each saliva sample. The saliva samples were centrifuged at 3,000 × g for 15 min at 4°C, and the supernatant was used for further analysis.…”

Section: Methodsmentioning

confidence: 99%

Individual Sweet Taste Perception Influences Salivary Characteristics After Orosensory Stimulation With Sucrose and Noncaloric Sweeteners

et al. 2022

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Emerging evidence points to a major role of salivary flow and viscoelastic properties in taste perception and mouthfeel. It has been proposed that sweet-tasting compounds influence salivary characteristics. However, whether perceived differences in the sensory properties of structurally diverse sweet-tasting compounds contribute to salivary flow and saliva viscoelasticity as part of mouthfeel and overall sweet taste perception remains to be clarified. In this study, we hypothesized that the sensory diversity of sweeteners would differentially change salivary characteristics in response to oral sweet taste stimulation. Therefore, we investigated salivary flow and saliva viscoelasticity from 21 healthy test subjects after orosensory stimulation with sucrose, rebaudioside M (RebM), sucralose, and neohesperidin dihydrochalcone (NHDC) in a crossover design and considered the basal level of selected influencing factors, including the basal oral microbiome. All test compounds enhanced the salivary flow rate by up to 1.51 ± 0.12 g/min for RebM compared to 1.10 ± 0.09 g/min for water within the 1st min after stimulation. The increase in flow rate was moderately correlated with the individually perceived sweet taste (r = 0.3, p < 0.01) but did not differ between the test compounds. The complex viscosity of saliva was not affected by the test compounds, but the analysis of covariance showed that it was associated (p < 0.05) with mucin 5B (Muc5B) concentration. The oral microbiome was of typical composition and diversity but was strongly individual-dependent (permutational analysis of variance (PERMANOVA): R2 = 0.76, p < 0.001) and was not associated with changes in salivary characteristics. In conclusion, this study indicates an impact of individual sweet taste impressions on the flow rate without measurable changes in the complex viscosity of saliva, which may contribute to the overall taste perception and mouthfeel of sweet-tasting compounds.

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“…Signaling pathways downstream of agonist-stimulated T2Rs have been examined, and found to involve gustducin [3, 4] and Gαi2 [14, 15]. In parallel, agonist-stimulation of T2Rs precipitates changes in Ca 2+ through Gβγ-mediated PLCβ [16], and Gαi activation reduces cAMP accumulation by inhibiting adenylate cyclase [17, 18]. Structural investigations suggest T2R46 precoupling to gustducin [19] as well as basal activity through T2R14-Gαi1 and T2R14-gustducin [20].…”

Section: Introductionmentioning

confidence: 99%

T2R14 mediated antimicrobial responses through interactions with CFTR

Gupte,

Singh,

Bhatia

et al. 2024

Preprint

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Bitter taste receptors (T2Rs), are a subset of G protein-coupled receptors (GPCRs) that play a key role in responding to microbial presence at epithelial surfaces. In epithelia, the activities of ion channels and transporters, and of T2Rs, mutually affect each other. The normal function of one such anion channel, cystic fibrosis transmembrane conductance regulator (CFTR), is essential for the maintenance of healthy epithelia, not just in the respiratory but in the digestive and reproductive system as well. Based on evidence that T2R14 activity is affected upon mutations in CFTR, we explored the possibility that T2R14 and CFTR directly interact in cell membranes. The biophysical interaction between these proteins was mapped to specific regions of the CFTR, and was dependent on agonist stimulation of T2R14. Further, T2R14 was found to couple to Gαq, in addition to the canonical Gαi, in response to bacterial and fungal quorum sensing molecules. Whether the interaction with CFTR affects T2R14 driven responses to microbial signals is under investigation.

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Effect of bitter compounds on amylase secretion in murine submandibular glands: Signaling pathway mechanisms

Cited by 6 publications

References 28 publications

Bitter taste receptor agonists mediate relaxation of human and rodent vascular smooth muscle

Bitter taste receptor agonists mediate relaxation of human and rodent vascular smooth muscle

Individual Sweet Taste Perception Influences Salivary Characteristics After Orosensory Stimulation With Sucrose and Noncaloric Sweeteners

T2R14 mediated antimicrobial responses through interactions with CFTR

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