Effect of Biotin on the Bacterial Formation of Glutamic Acid

Comparative Studies on the Mechanism of Microbial Glutamate Formation

“…In the present experiment, the activity obtained from biotin-rich cells of M, glutamicus was increased 80-fold. On the other hand, the activity in the extracts from biotin-rich and -poor cells of B. flavum was high, in accordance with our previous data (9). The ratio of the activities in the extracts from biotin-rich and -poor cells was as low as 2.…”

“…The methods for preparation of resting cells (7,8), and of cell free extracts (7), and for determination of enzyme activities (9) The abbreviations used in this paper include B. …”

Comparative Studies on the Mechanism of Microbial Glutamate Formation

“…Although the first half of their opinion is acceptable, the latter half is not because the yield of a-ketoglutarate formed from glucose in the absence of ammonia is substantially equal to that of glutamate formed in the presence of ammonia. Inhibitory effect of arsenite on oxidative metabolism of glucose As reported previously in B, flavum (13,14), oxidative metabolism of glucose was inhibited by arsenite and 1 mole of pyruvate was formed from 1 mole of glucose.…”

Fatty Acid Composition of Cell Wall-Cell Membrane Fraction From Brevibacterium Flavum

“…2247 (ATCC No. 14067), a typical strain of the so-called glutamate-producing bacteria, have clearly shown that the restriction of biotin concentration in the culture medium to some suboptimal level for growth results in the increase of cellular permeability to glutamate of the cells grown in this medium (1)(2)(3).…”