Effect of Biosynthesized ZnO Nanoparticles on Multi-Drug Resistant Pseudomonas Aeruginosa

Abstract

Synthesis of nanoparticles using the plants has several advantages over other methods due to the environmentally friendly nature of plants. Besides being environmentally friendly, the synthesis of nanoparticles using plants or parts of the plants is also cost effective. The present study focuses on the biosynthesis of zinc oxide nanoparticles (ZnO NPs) using the seed extract of Butea monsoperma and their effect on to the quorum-mediated virulence factors of multidrug-resistant clinical isolates of Pseudomonas aeruginosa at sub minimum inhibitory concentration (MIC). The synthesized ZnO NPs were characterized by different techniques, such as Fourier transform infra-red spectroscopy (FTIR), scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and transmission electron microscopy (TEM). The average size of the nanoparticles was 25 nm as analyzed by TEM. ZnO NPs at sub MIC decreased the production of virulence factors such as pyocyanin, protease and hemolysin for P. aeruginosa (p ≤ 0.05). The interaction of NPs with the P. aeruginosa cells on increasing concentration of NPs at sub MIC levels showed greater accumulation of nanoparticles inside the cells as analyzed by TEM.

Ecofriendly Synthesis of Silver Nanoparticles by Terrabacter humi sp. nov. and Their Antibacterial Application against Antibiotic-Resistant Pathogens

“…The MIC of the AgNPs synthesized by strain MAHUQ-38 T against E. coli were much lower than some other antimicrobial agents, including biosynthesized CuO-NPs and ZnO-NPs which were 0.5 mg/mL and 8 mg/mL, respectively, against E. coli [ 26 , 27 ]. The MICs of the AgNPs synthesized by strain MAHUQ-38 T against P. aeruginosa were also significantly lower than some other antimicrobial agents, including biogenic nanoparticles, which were in the range 0.375–1.6 mg/mL against P. aeruginosa [ 28 , 29 ]. The MBCs of biosynthesized AgNPs for both E. coli and P. aeruginosa were 50 μg/mL ( Figure 10 ), which was also much lower than those of some other antimicrobial agents [ 26 , 27 , 28 ].…”

Antimicrobial Activity of Zinc Oxide Nano/Microparticles and Their Combinations against Pathogenic Microorganisms for Biomedical Applications: From Physicochemical Characteristics to Pharmacological Aspects

“…ZnO NP mixtures at 25 nm (1600–3200 μg/mL) particularly had an antimicrobial activity against P. aeruginosa isolates from patients which were resistant to amikacin (30 μg), cefepime (30 μg), sparfloxacin (5 μg), piperacillin (100 μg), levofloxacin (5 μg), piperacilin-tazobactum (100/10 μg), imipenem (10 μg), tobramycin (10 μg), nitrofurantoin (300 μg), and ceftazidime (30 μg) [ 102 ].…”

Multifunctional bioactive core-shell electrospun membrane capable to terminate inflammatory cycle and promote angiogenesis in diabetic wound

“…Although, we could not test our fabricated nanofibrous membrane against P. aeruginosa. literature reports the effectiveness of both ZnO NP and OEO against this bacterium [ [53] , [54] , [55] , [56] ]. Many studies reported the effectiveness of OEO against multidrug resistance (MR) bacterial strain [ 57 ].…”