Effect of biomaterials hydrophobicity and roughness on biofilm development

De-la-Pinta, Iker; Cobos, Mónica; Ibarretxe, Julen; Montoya, Elizabeth; Eraso, Elena; Guraya, Teresa; Quindós, Guillermo

doi:10.1007/s10856-019-6281-3

Cited by 88 publications

(69 citation statements)

References 35 publications

(37 reference statements)

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“…The materials in which the final counts of CFU/mL were slightly higher were polyurethane and silicone that are very hydrophobic. These results are in accordance with other studies [31,32,33,34] that showed the importance of the hydrophobic effect of the biomaterial surface in the initial adhesion, where bacterial adhesion to the less hydrophobic materials were significantly lower than to the more hydrophobic ones (silicone). Higher roughness seems also to exert some effect in bacterial adhesion [31].…”

Section: Discussionsupporting

confidence: 93%

“…Thus, disinfection was more effective in titanium and borosilicate with the lowest hydrophobicity. Previous experiments carried out in our laboratory showed a lower ability of microorganisms to form biofilms on these materials [32]. The materials in which the final counts of CFU/mL were slightly higher were polyurethane and silicone that are very hydrophobic.…”

Section: Discussionmentioning

confidence: 93%

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Disinfectant Activity of A Portable Ultraviolet C Equipment

Guridi

Sevillano

Fuente

et al. 2019

IJERPH

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Healthcare-associated infections (HAIs) can be caused by microorganisms present in common practice instruments generating major health problems in the hospital environment. The aim of this work was to evaluate the disinfection capacity of a portable ultraviolet C equipment (UV Sanitizer Corvent® -UVSC-) developed to disinfect different objects. For this purpose, six pathogens causing HAIs: Acinetobacter baumannii, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans, were inoculated on slides and discs of different biomaterials (borosilicate, polycarbonate, polyurethane, silicone, Teflon and titanium) and exposed to ultraviolet C radiation. UVSC disinfection was compared with ethanol and chlorhexidine antimicrobial activities following the standards EN14561 and EN14562. Disinfection, established as a reduction of five logarithms from the initial inoculum, was achieved with the UVSC at 120 s of exposure time, with and without the presence of organic matter. The disinfectant effect was observed against S. aureus, P. aeruginosa, E. coli, B. subtilis and C. albicans (reduction >99.999%). Disinfection was also achieved with 70% ethanol and 2% chlorhexidine. As conclusion, UVSC was effective disinfecting the most contaminated surfaces assayed, being a promising alternative for disinfecting hospital materials and inanimate objects that cannot be immersed in liquid biocides, reducing the risk of pathogen transmission.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 93%

Disinfectant Activity of A Portable Ultraviolet C Equipment

Guridi

Sevillano

Fuente

et al. 2019

IJERPH

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“…In opposition, the higher hydrophobicity and roughness of SilRef may have hindered their initial antifouling activity. However, despite several studies reporting a correlation between the surface hydrophobicity and roughness and cell attachment [40,41], there is evidence that the biofilm formation induces changes in the surface properties [42,43]. These findings corroborate the higher antifouling activity of SilRef surfaces during the biofilm maturation stages.…”

Section: Discussionmentioning

confidence: 61%

Experimental Assessment of the Performance of Two Marine Coatings to Curb Biofilm Formation of Microfoulers

et al. 2020

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Biofilms formed on submerged marine surfaces play a critical role in the fouling process, causing increased fuel consumption, corrosion, and high maintenance costs. Thus, marine biofouling is a major issue and motivates the development of antifouling coatings. In this study, the performance of two commercial marine coatings, a foul-release silicone-based paint (SilRef) and an epoxy resin (EpoRef), was evaluated regarding their abilities to prevent biofilm formation by Cyanobium sp. and Pseudoalteromonas tunicata (common microfoulers). Biofilms were developed under defined hydrodynamic conditions to simulate marine settings, and the number of biofilm cells, wet weight, and thickness were monitored for 7 weeks. The biofilm structure was analyzed by confocal laser scanning microscopy (CLSM) at the end-point. Results demonstrated that EpoRef surfaces were effective in inhibiting biofilm formation at initial stages (until day 28), while SilRef surfaces showed high efficacy in decreasing biofilm formation during maturation (from day 35 onwards). Wet weight and thickness analysis, as well as CLSM data, indicate that SilRef surfaces were less prone to biofilm formation than EpoRef surfaces. Furthermore, the efficacy of SilRef surfaces may be dependent on the fouling microorganism, while the performance of EpoRef was strongly influenced by a combined effect of surface and microorganism.

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“…There is an increasing number of patients carrying biomedical devices. Severe infectious diseases associated with their use cause great morbidity and mortality, especially in critically ill people [84]. These types of GO-AgNP nanohybrids have found applications in different areas, as was previously mentioned.…”

Section: Antimicrobial Activity Assessmentmentioning

confidence: 82%

Graphene Oxide–Silver Nanoparticle Nanohybrids: Synthesis, Characterization, and Antimicrobial Properties

et al. 2020

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Drug resistance of pathogenic microorganisms has become a global public health problem, which has prompted the development of new materials with antimicrobial properties. In this context, antimicrobial nanohybrids are an alternative due to their synergistic properties. In this study, we used an environmentally friendly one-step approach to synthesize graphene oxide (GO) decorated with silver nanoparticles (GO–AgNPs). By this process, spherical AgNPs of average size less than 4 nm homogeneously distributed on the surface of the partially reduced GO can be generated in the absence of any stabilizing agent, only with ascorbic acid (L-AA) as a reducing agent and AgNO3 as a metal precursor. The size of the AgNPs can be controlled by the AgNO3 concentration and temperature. Smaller AgNPs are obtained at lower concentrations of the silver precursor and lower temperatures. The antimicrobial properties of nanohybrids against Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa, Gram-positive Staphylococcus aureus, and the yeast Candida albicans were found to be concentration- and time-dependent. C. albicans and S. aureus showed the highest susceptibility to GO–AgNPs. These nanohybrids can be used as nanofillers in polymer nanocomposites to develop materials with antimicrobial activity for applications in different areas, and another potential application could be cancer therapeutic agents.

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Effect of biomaterials hydrophobicity and roughness on biofilm development

Cited by 88 publications

References 35 publications

Disinfectant Activity of A Portable Ultraviolet C Equipment

Disinfectant Activity of A Portable Ultraviolet C Equipment

Experimental Assessment of the Performance of Two Marine Coatings to Curb Biofilm Formation of Microfoulers

Graphene Oxide–Silver Nanoparticle Nanohybrids: Synthesis, Characterization, and Antimicrobial Properties

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