Effect of biologically synthesized copper oxide nanoparticles on metabolism and antioxidant activity to the crop plants Solanum lycopersicum and Brassica oleracea var. botrytis

Singh, Arun Kumar; Singh, Neeraj; Hussain, Imtiyaz; Singh, Himani

doi:10.1016/j.jbiotec.2017.09.016

Cited by 132 publications

(45 citation statements)

References 56 publications

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“…and phytochemicals like indoles which possess detoxifying property 23 , 24 . Nevertheless, nanoparticles synthesized from Brassica species have been reported earlier 25 – 30 , a comparative analysis of different Brassicaceae members for their reducing capacity, for controlled synthesis of AgNPs and to explore better performing varieties with higher antimicrobial potential is lacking. Hence, in the present study owing to the presence of antioxidants and other detoxifying chemicals, the NPs synthesized from B. oleracea has been hypothesized to play role as an effective antimicrobial and anticancer tools.…”

Section: Introductionmentioning

confidence: 99%

Eco friendly silver nanoparticles synthesis by Brassica oleracea and its antibacterial, anticancer and antioxidant properties

Ansar

Tabassum

Aladwan

et al. 2020

Sci Rep

116

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Production of environmentally amenable silver nanoparticles (AgNPs) has garnered the interest of the scientific community owing to their broad application primarily in the field of optronics, sensing and extensively in pharmaceuticals as promising antioxidant, antimicrobial and anticancer agents. The current study emphases on production of ecofriendly silver nanoparticles from Brassica oleracea (BO) and investigated their antibacterial, anticancer and antioxidant activity. The characteristics of synthesized BO-AgNPs were studied by ultraviolet–visible spectroscopy, particle size analysis, electro kinetic/zeta potential analysis, and Transmission electron microscope (TEM). A distinctive absorption maximum at 400 nm confirmed the formation of BO-AgNPs and data on TEM analysis have shown that the synthesized nanoparticles were predominantly spherical in shape. The BO-AgNPs obtained were assessed for antibacterial, antioxidant, and cytotoxic ability in MCF-7 cells. The antibacterial activity expressed was maximum against Staphylococcus epidermidis (Gram positive) and Pseudomonas aeruginosa (Gram negative) with DIZ of 14.33 ± 0.57 and 12.0 ± 0.20 mm respectively. Furthermore, the ability of the synthesized green nanoparticles to scavenge free radicals revealed a strong antioxidant activity. The cytotoxicity increased proportionately with increasing concentration of the green synthesized BO-AgNPs with maximum effect at 100 μg/ml and IC50 of 55 μg/ml. In conclusion, the data obtained in the study is reflective of the role of BO-AgNPs as potential and promising antimicrobial agent against bacterial infections and potential anticancer agent in cancer therapy.

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Section: Introductionmentioning

confidence: 99%

Eco friendly silver nanoparticles synthesis by Brassica oleracea and its antibacterial, anticancer and antioxidant properties

Ansar

Tabassum

Aladwan

et al. 2020

Sci Rep

116

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show abstract

“…Therefore, there is a great need to investigate the effect of NPs on plants including their uptake, translocation as well as their potential toxicity. It has been reported that the impact of NPs on plants can be diverse and depends on the type of NPs, their physicochemical properties (e.g., particle size, shape or surface properties), concentration, time exposure as well as plant species [10,11,12,13,14,15]. For example, Yin et al [16] found that in Lolium multiflorum roots, accumulation of silver NPs (AgNPs) of 6 nm in diameter was higher than for 25 nm.…”

Section: Introductionmentioning

confidence: 99%

Effect of Nanoparticles Surface Charge on the Arabidopsis thaliana (L.) Roots Development and Their Movement into the Root Cells and Protoplasts

Milewska‐Hendel

Zubko

Stróż

et al. 2019

IJMS

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Increasing usage of gold nanoparticles (AuNPs) in different industrial areas inevitably leads to their release into the environment. Thus, living organisms, including plants, may be exposed to a direct contact with nanoparticles (NPs). Despite the growing amount of research on this topic, our knowledge about NPs uptake by plants and their influence on different developmental processes is still insufficient. The first physical barrier for NPs penetration to the plant body is a cell wall which protects cytoplasm from external factors and environmental stresses. The absence of a cell wall may facilitate the internalization of various particles including NPs. Our studies have shown that AuNPs, independently of their surface charge, did not cross the cell wall of Arabidopsis thaliana (L.) roots. However, the research carried out with using light and transmission electron microscope revealed that AuNPs with different surface charge caused diverse changes in the root’s histology and ultrastructure. Therefore, we verified whether this is only the wall which protects cells against particles penetration and for this purpose we used protoplasts culture. It has been shown that plasma membrane (PM) is not a barrier for positively charged (+) AuNPs and negatively charged (−) AuNPs, which passage to the cell.

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“…According to the previous study, the chlorophyll activity may be influenced both by the mechanical coating of NPs on leaf surface or by the accumulated Cu within plant [34,38,39]. Moreover, chloroplasts is one of the sources of ROS generation, the reduction of chlorophyll in plants upon exposure to CuO-NPs may attributed directly to oxidative stress [40]. Carotenoids are known to be light harvesting pigments by absorbing photons and transferring the excitation energy to chlorophyll, which eventually reaches the reaction center, they also act as potent quenchers of ROS, particularly singlet oxygen ( 1 O 2 ) by intercepting the triplet-chlorophyll [20].…”

Section: Gas Exchange Photosynthetic Pigments Concentration and Chlorophyll Fluorescencementioning

confidence: 94%

Dose-Dependent Physiological and Transcriptomic Responses of Lettuce (Lactuca sativa L.) to Copper Oxide Nanoparticles—Insights into the Phytotoxicity Mechanisms

Xiong

Zhang

Kang

et al. 2021

IJMS

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Understanding the complex mechanisms involved in plant response to nanoparticles (NPs) is indispensable in assessing the environmental impact of nano-pollutants. Plant leaves can directly intercept or absorb NPs deposited on their surface; however, the toxicity mechanisms of NPs to plant leaves are unclear. In this study, lettuce leaves were exposed to copper oxide nanoparticles (CuO-NPs, 0, 100, and 1000 mg/L) for 15 days, then physiological tests and transcriptomic analyses were conducted to evaluate the negative impacts of CuO-NPs. Both physiological and transcriptomic results demonstrated that CuO-NPs adversely affected plant growth, photosynthesis, and enhanced reactive oxygen species (ROS) accumulation and antioxidant system activity. The comparative transcriptome analysis showed that 2270 and 4264 genes were differentially expressed upon exposure to 100 and 1000 mg/L CuO-NPs. Gene expression analysis suggested the ATP-binding cassette (ABC) transporter family, heavy metal-associated isoprenylated plant proteins (HIPPs), endocytosis, and other metal ion binding proteins or channels play significant roles in CuO-NP accumulation by plant leaves. Furthermore, the variation in antioxidant enzyme transcript levels (POD1, MDAR4, APX2, FSDs), flavonoid content, cell wall structure and components, and hormone (auxin) could be essential in regulating CuO-NPs-induced stress. These findings could help understand the toxicity mechanisms of metal NPs on crops, especially NPs resulting from foliar exposure.

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Effect of biologically synthesized copper oxide nanoparticles on metabolism and antioxidant activity to the crop plants Solanum lycopersicum and Brassica oleracea var. botrytis

Cited by 132 publications

References 56 publications

Eco friendly silver nanoparticles synthesis by Brassica oleracea and its antibacterial, anticancer and antioxidant properties

Eco friendly silver nanoparticles synthesis by Brassica oleracea and its antibacterial, anticancer and antioxidant properties

Effect of Nanoparticles Surface Charge on the Arabidopsis thaliana (L.) Roots Development and Their Movement into the Root Cells and Protoplasts

Dose-Dependent Physiological and Transcriptomic Responses of Lettuce (Lactuca sativa L.) to Copper Oxide Nanoparticles—Insights into the Phytotoxicity Mechanisms

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