The present work was conducted on a 1 DI CI engine, on which neat diesel, neem biodiesel and polanga biodiesel and ethanol fuel were tested by varying the load on the engine setup at various blend ratios such as: -diesel fuel 100% (D100), biodiesel neem 100% (N100), biodiesel polanga 50% blended with diesel 50% (P50), and ethanol 5% blended with diesel 95% (E5).The research carried was to compar the performance-emission characteristics of various blend samples w.r.t neat diesel fuel. The performance results show that, the BTE of N100, P50 fuel blends was less than E5 blend, as compared to neat diesel, whereas, the BSFC of D100, E5 blend had a decreasing nature than N100 and P50 blend. The CO emissions among the biofuel blends was maximum for N100 and then P50 blend but the least was for E5 blend w.r.t neat diesel. Also, the UHC emission for N100, P50 and E5 blends had a decreasing trend than neat diesel fuel. The D100 fuel had a maximum NOx emission in comparison to others and the least was by E5 blend. The CO2 emission of N100 and D100 was the highest than P50 and E5 blends during the operation. The unused O2for N100 fuel was the least than other fuel samples and the maximum was for E5 blend. The biofuel blends being used here had an effective outcome which can be utilised as an substitute for neat diesel.