Effect of Biocontrol Agent Pseudomonas fluorescens 2P24 on Soil Fungal Community in Cucumber Rhizosphere Using T-RFLP and DGGE

GuanPeng, Gao; Yin, Danhan; Sheng-ju, Chen; Xia, Fei; Yang, Jie; Li, Qing X.; Wang, Wei

doi:10.1371/journal.pone.0031806

Cited by 60 publications

(34 citation statements)

References 50 publications

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“…Some biotic factors such as Bacillus species can attack primary zoospores when P. brassicae germinates and moves into the soil (Dixon, 2009), thereby protecting canola from infection. In addition, the use of a biocontrol agent could also have impacts on the bacterial communities of the rhizosphere soil in plants (Gao et al, 2012), including Bacillus sp., which may work as a biocontrol agent to reduce the severity of plant disease. However, little is known about the relationships between P. brassicae and microflora in the soil (Dixon, 2009), or the influence of different biocontrol agents on the bacterial communities.…”

Section: Biocontrol Science and Technologymentioning

confidence: 99%

Biocontrol effect ofTrichoderma harzianumT4 on brassica clubroot and analysis of rhizosphere microbial communities based on T-RFLP

Zhao

Cheng

et al. 2015

Biocontrol Science and Technology

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2015): Biocontrol effect of Trichoderma harzianum T4 on brassica clubroot and analysis of rhizosphere microbial communities based on T-RFLP, Biocontrol Science and Technology, Abstract Clubroot is a serious threat to cruciferous crops in Shanghai, China, and Trichoderma harzianum T4 (T4) has shown good efficiency for its control. In this study, T4 was applied alone or in combination with the fungicide cyazofamid or Bacillus subtilis XF-1, respectively, in a pot experiment to determine its efficiency of controlling clubroot.The control efficiency of T4 (79.3%) was significantly higher than that of XF-1 (63.1%, p < 0.05), and was similar to that of cyazofamid (93.2%, p > 0.05). Furthermore, T4 could promote the growth of the Chinese cabbage Brassica, particularly with respect to its fresh A c c e p t e d M a n u s c r i p t Downloaded by [University of Nebraska, Lincoln] at 00:05 26 August 2015 Xuexin Yu et al.weight. These results indicated that T4 could be used as a green biofungicide to not only control the incidence of clubroot but also to increase the production of vegetables. The structure of microbial communities changed substantially when T4 was applied to the soil, as revealed by a non-metric multidimensional scaling (nMDS) test. T4 could not only reduce the average abundance of Bacillus sp. and Acidiphilium sp. (terminal restriction fragment 231-TRF231, the most dominant fragment) but could also increase the average abundance of Streptococcus sp. (TRF307, the second most dominant fragment). Therefore, the high control efficiency of T4 against clubroot results not only from its ability to directly inhibit spore germination of P. brassicae but also from its ability to regulate the microbial communities in the rhizosphere soil of B. chinensis, which could then inhibit the growth of P. brassicae in soil indirectly.

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Section: Biocontrol Science and Technologymentioning

confidence: 99%

Biocontrol effect ofTrichoderma harzianumT4 on brassica clubroot and analysis of rhizosphere microbial communities based on T-RFLP

Zhao

Cheng

et al. 2015

Biocontrol Science and Technology

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“…has the potential to modify the soil/compost microbiota, its impact on non-target micro-organisms has to be studied [13]. Several studies have been conducted to investigate non-target effects of BCAs on soil microbial communities [14, 15] and no studies have been conducted on compost/peat substrates. A key to progress in the field of biological control is focused on the dynamic interactions between the biocontrol agent, the pathogen and rhizosphere [16].…”

Section: Introductionmentioning

confidence: 99%

Relationship of microbial communities and suppressiveness of Trichoderma fortified composts for pepper seedlings infected by Phytophthora nicotianae

et al. 2017

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The understanding of the dynamic of soil-borne diseases is related to the microbial composition of the rhizosphere which is the key to progress in the field of biological control. Trichoderma spp. is commonly used as a biological control agent. The use of next generation sequencing approaches and quantitative PCR are two successful approaches to assess the effect of using compost as substrate fortified with two Trichoderma strains (Trichoderma harzianum or Trichoderma asperellum) on bacterial and fungal communities in pepper rhizosphere infected with Phytophthora nicotianae. The results showed changes in the bacterial rhizosphere community not attributed to the Trichoderma strain, but to the pathogen infection, while, fungi were not affected by pathogen infection and depended on the type of substrate. The Trichoderma asperellum fortified compost was the most effective combination against the pathogen. This could indicate that the effect of fortified composts is greater than compost itself and the biocontrol effect should be attributed to the Trichoderma strains rather than the compost microbiota, although some microorganisms could help with the biocontrol effect.

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“…With regard to pathogenicity, most of the research has focused on the survival and transport of E. coli O157:H7 in soil, while the potential effect of E. coli O157:H7 on the microbial community in agricultural soil is still unknown. Thus, it is important to address the displacement of indigenous microorganisms by inoculants and its potential effects on the soil microcosm (28).…”

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Interaction between the Microbial Community and Invading Escherichia coli O157:H7 in Soils from Vegetable Fields

Yao

Wang

et al. 2014

Appl Environ Microbiol

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The survival of Escherichia coli O157:H7 in soils can contaminate vegetables, fruits, drinking water, etc. However, data on the impact of E. coli O157:H7 on soil microbial communities are limited. In this study, we monitored the changes in the indigenous microbial community by using the phospholipid fatty acid (PLFA) method to investigate the interaction of the soil microbial community with E. coli O157:H7 in soils. Simple correlation analysis showed that the survival of E. coli O157:H7 in the test soils was negatively correlated with the ratio of Gram-negative (G ؊ ) to Gram-positive (G ؉ ) bacterial PLFAs (G ؊ /G ؉ ratio). In particular, levels of 14 PLFAs were negatively correlated with the survival time of E. coli O157:H7. The contents of actinomycetous and fungal PLFAs in the test soils declined significantly (P, <0.05) after 25 days of incubation with E. coli O157:H7. The G ؊ /G ؉ ratio declined slightly, while the ratio of bacterial to fungal PLFAs (B/F ratio) and the ratio of normal saturated PLFAs to monounsaturated PLFAs (S/M ratio) increased, after E. coli O157:H7 inoculation. Principal component analysis results further indicated that invasion by E. coli O157:H7 had some effects on the soil microbial community. Our data revealed that the toxicity of E. coli O157:H7 presents not only in its pathogenicity but also in its effect on soil microecology. Hence, close attention should be paid to the survival of E. coli O157:H7 and its potential for contaminating soils.

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Effect of Biocontrol Agent Pseudomonas fluorescens 2P24 on Soil Fungal Community in Cucumber Rhizosphere Using T-RFLP and DGGE

Cited by 60 publications

References 50 publications

Biocontrol effect ofTrichoderma harzianumT4 on brassica clubroot and analysis of rhizosphere microbial communities based on T-RFLP

Biocontrol effect ofTrichoderma harzianumT4 on brassica clubroot and analysis of rhizosphere microbial communities based on T-RFLP

Relationship of microbial communities and suppressiveness of Trichoderma fortified composts for pepper seedlings infected by Phytophthora nicotianae

Interaction between the Microbial Community and Invading Escherichia coli O157:H7 in Soils from Vegetable Fields

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