To solve the problem that the repair material of the bridge's expansion joint anchorage area is prone to debonding from the other bridge components, polyurethane concrete was designed and prepared, and the bonding performance of polyurethane concrete was tested by the bond splitting test, bond direct shear test, bond oblique shear test, and steel reinforcement pull-out test. The results show that the interfacial bond strength of polyurethane concrete and cement concrete can be improved by increasing the interfacial roughness. The bond splitting strength increases by up to 91.4%, the bond direct shear strength increases by up to 55.2%, and the bond oblique shear strength increases by up to 28.9%. The bond strength between polyurethane concrete and cement concrete are minimally influenced by temperature. An increase in the diameter of the reinforcement or the length of the bonded anchorage can increase the ultimate pull-out strength of the polyurethane concrete and the steel reinforcement. Polyurethane concrete is a novel composite material with excellent bonding performance with cement concrete and steel reinforcement, which can be widely used in practical projects, such as repairing bridge expansion joint anchorage areas in the future.