Effect of bile acids on electrical properties of rat colon: evaluation of an in-vitro model for secretion.

Abstract

SUMMARY A preparation of rat colon mucosa has been evaluated as a useful model to study effects of bile acids on the changes in short-circuit current and transmural electrical potential difference (pd) which others have associated with alterations in electrolyte transport. Using this preparation, it was found that bile acids were effective in increasing short-circuit current and pd when applied to the serosal, but not the mucosal, surface of the tissue. Furthermore, taurine-conjugated cholic acid, which has no demonstrable effect on the colon in vivo, was found to increase short-circuit current and pd significantly in the in-vitro preparation. These data indicate the limitations of the in-vitro model in studying the mechanism of bile acid-stimulated intestinal secretion.

The Effect of Gallotannins and (+)-Catechin on the Stimulated Fluid Secretion on the Colon, Following a Rhein Perfusion in Guinea Pigs1

“…Rhein has been shown to inhibit Na-K-ATPase (22) in the enterocyte and to inhibit active sugar transport in the colon (13). Other effects on hydraulic permeability and paracellular permeability of intestinal membranes are described (23,24). Further studies about the influence of tannins on these processes are necessary.…”

Intestinal filtration as a consequence of increased mucosal hydraulic permeability

“…Intestinal filtration as a consequence of increased mucosat hydraulic permeability: laxative acting bile acids increase the electrical conductance and the hydraulic permeability of the intestinal mucosa for a variety of water-soluble compounds (Binder and Rawlins, t973;Wail and Baker, 1974;Feldman et al, t975;Wanitschke and Soergel, t975;Schwiter et al, 1975;Dobbins and Binder, 1976;Nell et al, 1976;Chadwick et al, 1977;Goerg, 1979). This effect has also been demonstrated for diphenolic laxatives (Nell etal., 1973a, b;.…”

Efecto del ácido deoxicólico sobre la regulación del transporte iónico intestinal en la rata

“…Por otra parte, se ha observado un efecto de los ácidos biliares sobre el transporte hidrosalino al actuar sobre el polo seroso de la célula epitelial del intestino 2,7,8 . Al respecto, Berant et al 9 .…”