Effect of beta-lactamase inhibitors on the activities of various beta-lactam agents against anaerobic bacteria

Wexler, Hannah M.; Molitoris, E; Finegold, Sydney M.

doi:10.1128/aac.35.6.1219

Cited by 32 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Controversy exists over the activity of ceftizoxime against the B. fragilis group. Some researchers have reported that ceftizoxime is more active than cefoxitin (2,4,15); however, others have disagreed (9,13,19,20). This discrepancy may be due to the different methods used for in vitro susceptibility testing and the significant inoculum effect observed with ceftizoxime (1)(2)(3)(4) …”

Section: Discussionmentioning

confidence: 68%

“…P-lactamase production was determined by the nitrocefin method using nitrocefin disks (Cefinase disks; BBL Microbiology Systems, Cockeysville, Md.). Finegold et al (11,20) has suggested that to facilitate comparison between different studies, presentation of data should take into consideration two factors, the various breakpoints for different drugs that have been used in the past and the clustering of a significant percentage of susceptibility test results at or near the accepted breakpoints with a number of drugs and particularly the P-lactam agents. Had we considered breakpoints of 64 and 128 ,ug/ml, resistance rates would have been 19 and 12% for piperacillin and 31 and 20% for ticarcillin.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Survey of Bacteroides fragilis group susceptibility patterns in Canada

Bourgault

Lamothe

Hoban

et al. 1992

Antimicrob Agents Chemother

View full text Add to dashboard Cite

The in vitro activities of penicillin, clindamycin, chloramphenicol, metronidazole, piperacillin, piperacillintazobactam, ticarcillin, ticarcillin-clavulanate, ampicillin-sulbactam, cefoxitin, ceftizoxime, cefotetan, moxalactam, and imipenem against 348 Bacteroidesfragilis group isolates collected from six Canadian cities during 1990 were determined by the National Committee for Clinical Laboratory Standards (NCCLS) agar dilution technique. All isolates were susceptible to chloramphenicol, metronidazole, piperacillin-tazobactam, and imipenem. For the other antibiotics tested, the following resistance rates were observed: penicillin, 97%; clindamycin, 9%; piperacillin, 19%; ticarcillin, 31%; ticarcillin-clavulanate, 0.28%; ampicillin-sulbactam, 0.85%; cefoxitin, 26%; ceftizoxime, 15%; cefotetan, 53%; and moxalactam, 17%. Susceptibility proffles to beta-lactam antibiotics varied among the different species tested: B. fragilis and Bacteroides vulgatus demonstrated lower resistance rates than Bacteroides distasonis and indole-positive Bacteroides thetaiotaomicron and Bacteroides ovatus. Ceftizoxime results should be interpreted cautiously, because the MICs obtained with the recommended NCCLS control strain were lower than expected.The Bacteroidesfragilis group is the most important group of anaerobic gram-negative pathogens. Until recently, the susceptibility patterns of these organisms had been assumed to be relatively stable and routine susceptibility testing had not been advocated. However, in recent years, the in vitro antimicrobial susceptibility patterns of these organisms seem to have undergone gradual changes (5,6,9,10,12,18

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Methodsmentioning

confidence: 99%

Survey of Bacteroides fragilis group susceptibility patterns in Canada

Bourgault

Lamothe

Hoban

et al. 1992

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…In Japan, the daily doses of ß-lactams usually used for adult orofacial odontogenic infections are regulated by Health and Welfare of Japan. For example, the doses for an adult (with 60 kg weight) are as follows: oral-ampicillin, 1 g; intravenous-ampicillin, 2 g; cephalexin, 750 mg; cefazolin, 1 g; cefmetazole, 2 g; cefpodoxime, 200 mg; cefdinir, 300 mg. Prevotella, Porphyromonas, and Fusobacterium, 33,34 and the occurrence of side effects is lower than with other antibiotics. In addition, cost should be taken into account.…”

Section: Discussionmentioning

confidence: 99%

Past administration of β-lactam antibiotics and increase in the emergence of β-lactamase–producing bacteria in patients with orofacial odontogenic infections

Kuriyama¹,

Nakagawa²,

Karasawa³

et al. 2000

Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, an

View full text Add to dashboard Cite

“…Clindamycin was active against 98% of the strains tested. Because of generalized hospital use of this compound in the United States, clindamycin resistance may occur (22) and should be monitored for. Temafloxacin was active against 95% of all strains at c4 ,ug/ml, but MICs clustered around the breakpoint.…”

mentioning

confidence: 99%

Susceptibilities of 540 anaerobic gram-negative bacilli to amoxicillin, amoxicillin-BRL 42715, amoxicillin-clavulanate, temafloxacin, and clindamycin

Appelbaum

Spangler

Shiman

et al. 1992

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Agar dilution MIC testing of amoxicillin, amoxicillin-BRL 42715, amoxicillin-clavulanate, temafloxacin, and clindamycin against 496 ,-lactamase-producing anaerobic gram-negative rods revealed MICs for 90o% of the strains tested of 256.0 (amoxicillin), 2.0 (amoxicillin-BRL 42715 and amoxicillin-clavulanate), and 4.0 (temafloxacin and clindamycin) ,ug/ml. Amoxicillin, temafloxacin, and clindamycin inhibited all 44 13-lactamase-negative strains (MICs for 90%o of the strains tested, <2.0 ,ug/ml). BRL 42715 will not be developed, but temafloxacin merits clinical evaluation.,B-Lactamase production and concomitant resistance to many ,B-lactams are usually encountered in the Bacteroides fragilis group but have been increasingly reported in other anaerobic gram-negative rods (2,6,7,13,14). We have examined the susceptibilities of 304 B. fragilis strains; 171 non-B. fragilis group Bacteroides, Prevotella, and Porphyromonas species (142 were P-lactamase positive); and 65 Fusobacterium species (50 were 1-lactamase positive) to amoxicillin, amoxicillin-BRL 42715 (a potent new ,B-lactamase inhibitor [8,9,17,23]), amoxicillin-clavulanate, temafloxacin (10,11,16,19), and clindamycin.Organisms were selected from clinical isolates collected over the past 4 years from diverse sources as previously described (2-6, 13, 14). Prior to being tested, strains were identified (1, 12, 20) as described previously. P-Lactamase production was tested by the nitrocefin disk method (BBL Microbiology Systems, Cockeysville, Md.) (4). MICs of amoxicillin, amoxicillin-clavulanate, amoxicillin-BRL 42715 (SmithKline Beecham Pharmaceuticals, Brockham Park, Betchworth, United Kingdom), temafloxacin (Abbott Laboratories, Chicago, Ill.), and clindamycin (The Upjohn Co., Kalamazoo, Mich.) were determined by agar dilution (2) extracts were standardized to contain similar units of activity against nitrocefin.In Tables 1 and 2, percent susceptibility rates of amoxicillin with and without inhibitors are presented at breakpoints of 2.0, 4.0, and 8.0 ,ugIml, respectively. Susceptibility data for ,B-lactamase-producing strains are shown in Table 1. Against all 3-lactamase-positive strains, addition of BRL 42715 to amoxicillin lowered the MICs for 50 and 90% of the strains tested (MlC50 and MIC90, respectively) from 32 and 256 to 0.25 and 2 ,ug/ml, respectively, and 99% of the strains were susceptible at all three amoxicillin breakpoints (27% of the strains were susceptible to amoxicillin at the NCCLS breakpoint of 4 ,ug/ml). Addition of clavulanate to amoxicillin lowered the MIC50 and MIC90 to 0.5 and 2.0 ,ug/ml, respectively, with 97% of the strains susceptible at the NCCLS amoxicillin breakpoint of 8 ,ug/ml (18). Temafloxacin and clindamycin showed good activity against 13-lactamase-producing strains (95 and 98%, respectively, were susceptible at .4 ,ug/ml), but clustering near the breakpoint was seen with both compounds.All ,B-lactamase-negative strains were susceptible to amoxicillin, temafloxacin, and clindamycin (Table 2), and many were susceptible to the...

show abstract

Effect of beta-lactamase inhibitors on the activities of various beta-lactam agents against anaerobic bacteria

Cited by 32 publications

References 14 publications

Survey of Bacteroides fragilis group susceptibility patterns in Canada

Survey of Bacteroides fragilis group susceptibility patterns in Canada

Past administration of β-lactam antibiotics and increase in the emergence of β-lactamase–producing bacteria in patients with orofacial odontogenic infections

Susceptibilities of 540 anaerobic gram-negative bacilli to amoxicillin, amoxicillin-BRL 42715, amoxicillin-clavulanate, temafloxacin, and clindamycin

Contact Info

Product

Resources

About