Effect of berberine on expression of hepatocyte nuclear factor-4α in rats with fructose-induced insulin resistance

Gao, Zhiqiang; Leng, San-Hua; Lu, Fanghong; Xie, M.Z.; Xu, Li; Wang, Kaifu

doi:10.1007/s11596-008-0307-2

Cited by 11 publications

(11 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our finding in this study revealed that FDW consumption, in a month, induced insulin resistance index (IRI), hyperinsulinemia a serum proinflammatory cytokines values such as IL6 and TNF in male rats. These results were confirmed by previous studies indicating that fructose-fed and FDW cause central obesity, insulin resistance, and hyperinsulinemia to increase in rats [ 4 ]. Previous studies indicated that TNF serum concentration elevated and altered insulin signaling in skeletal muscles cell membranes in insulin resistance disorders (type II diabetes and MS), but Artemisia dracunculus L. extract improved insulin action by increasing insulin signaling in skeletal muscle [ 16 ].…”

Section: Discussionsupporting

confidence: 90%

“…In addition, there are associations among insulin resistance, abdominal obesity, and serum immune markers such as IL6 and TNF α [ 3 ]. Moreover, metabolic syndrome related to insulin resistance (IR) is a state in that insulin is higher than normal concentration and it plays an important role in the pathophysiology of most common human diseases such as type 2 diabetes mellitus, hypertension, and coronary heart disease [ 4 ]. A mixture of several botanical medicines is used to treat diabetes mellitus and insulin resistance accompanied by hypertension in fructose-fed rats (FFR) [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Nociceptive and Anti-Inflammatory Effects ofArtemisia dracunculusL. Aqueous Extract on Fructose Fed Male Rats

Reza

Mirshekari

Saberi

2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Aim & Objective. Artemisia dracunculus L. (Tarragon) species have been used as a traditional medicine. The present study was designed to evaluate the nociceptive and anti-inflammatory effects of A. dracunculus L. leaf aqueous extract on fructose drinking water (FDW) in male rats. Materials & Methods. Forty-eight Wistar-albino male rats weighing 200–250 g were divided into control (C), control extract (CE), FDW, and FDWE groups (n = 12). Group C did not receive any agents; Group CE did 100 mg/kg A. dracunculus L. aqueous extract on a daily basis for duration of four weeks. FDW Group received fructose drinking water (10%, weight/volume) but did not receive any agents during trial period. FDWE group received 100 mg/kg A. dracunculus L. aqueous extract during trial period. At the end of experiment, a biphasic pain response was induced following interplanetary injection of formalin (50 µL, 1%). Obtained data were analyzed using SPSS software version 17 and using ANOVA and Tukey post hoc tests. Results were expressed as mean ± SE. Statistical differences were considered significant at P < 0.05. Results. Our findings revealed that acute and chronic pain scores in FDW group are significantly higher than other ones and A. dracunculus L. aqueous extract causes significant decreasing of this parameter in FDWE group (P < 0.001). Moreover, IL6 and TNF values in this group were significantly decreased compared to FDW group (P < 0.05). Conclusion. Results in the present study show that FDW causes the pain response score to increase and cause proinflammatory cytokines in rat model but A. dracunculus L. leaf aqueous extract improves values of these parameters.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The Nociceptive and Anti-Inflammatory Effects ofArtemisia dracunculusL. Aqueous Extract on Fructose Fed Male Rats

Reza

Mirshekari

Saberi

2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

show abstract

“…It has also been demonstrated that inhibiting phosphorylation of IKK β might be a cofactor of berberine in achieving its anti-inflammation and insulin-resistance-improving effects [22]. The latest study also proved that berberine could inhibit fructose-induced insulin resistance in rats possibly by increasing the expression HNF-4 α in liver [23]. HNF-4 α is a positive regulator of PEPCK so an increase of HNF-4 α would result in increased gluconeogenesis.…”

Section: Discussionmentioning

confidence: 99%

The Characterization of High‐Fat Diet and Multiple Low‐Dose Streptozotocin Induced Type 2 Diabetes Rat Model

Zhang

et al. 2008

Journal of Diabetes Research

500

405

View full text Add to dashboard Cite

Aim. Based on the previously established method, we developed a better and stable animal model of type 2 diabetes mellitus by high-fat diet combined with multiple low-dose STZ injections. Meanwhile, this new model was used to evaluate the antidiabetic effect of berberine. Method. Wistar male rats fed with regular chow for 4 weeks received vehicle (control groups), rats fed with high-fat diet for 4 weeks received different amounts of STZ once or twice by intraperitoneal injection (diabetic model groups), and diabetic rats were treated with berberine (100 mg/kg, berberine treatment group). Intraperitoneal glucose tolerance test and insulin tolerance test were carried out. Moreover, fasting blood glucose, fasting insulin, total cholesterol, and triglyceride were measured to evaluate the dynamic blood sugar and lipid metabolism. Result. The highest successful rate (100%) was observed in rats treated with a single injection of 45 mg/kg STZ, but the plasma insulin level of this particular group was significantly decreased, and ISI has no difference compared to control group. The successful rate of 30 mg/kg STZ twice injection group was significantly high (85%) and the rats in this group presented a typical characteristic of T2DM as insulin resistance, hyperglycemia, and blood lipid disorder. All these symptoms observed in the 30 mg/kg STZ twice injection group were recovered by the treatment of berberine. Conclusion. Together, these results indicated that high-fat diet combined with multiple low doses of STZ (30 mg/kg at weekly intervals for 2 weeks) proved to be a better way for developing a stable animal model of type 2 diabetes, and this new model may be suitable for pharmaceutical screening.

show abstract

“…In cancer cells, berberine up-regulated multidrug-resistance transporter expression (Lin et al, 1999), and the induction of P-glycoprotein is partially regulated by PXR (Harmsen et al, 2010). Berberine also prevented fructose-induced insulin resistance in rat islet cells by promoting the expression of HNF4α (Gao et al, 2008). However, information about effects of berberine on these nuclear receptors as well as whether drug processing genes are altered after in vivo exposure is limited.…”

Section: Introductionmentioning

confidence: 99%

Dose–response of berberine on hepatic cytochromes P450 mRNA expression and activities in mice

Guo¹,

Pope²,

Cheng³

et al. 2011

Journal of Ethnopharmacology

View full text Add to dashboard Cite

Ethnopharmacological relevance Berberine is an isoquinoline alkaloid isolated from the root and bark of plants such as goldenseal, Berberis, and Chinese goldthread. Berberine-containing crude drugs have been used as an antimicrobial remedy against gastrointestinal infections for thousands of years. It is also widely used in Asian countries for diabetes, hypertension, and hypercholesterolemia therapy. Aim of the Study Potential drug-drug interactions are of concern because of the wide usage of berberine. A few studies have reported interactions between berberine and cytochromes P450 (CYPs) in vitro, but little is known about whether berberine influences CYPs in vivo, especially after repeated administration. In this study, eight-week-old male C57BL/6 mice were given berberine orally (0,10, 30, 100, 300 mg/kg, i.g., daily for 14 days), and the effect of berberine on over 20 major Cyps and related nuclear receptors in mice livers were examined at both the mRNA and enzyme activity levels. Results In general, liver function of mice treated with various doses of berberine had no significant change, and repeated oral administration of the 3 lower doses of berberine for 14 days did not affect the expression of genes examined. However, after the highest dose of berberine (300 mg/kg), Cyp3a11 and Cyp3a25 mRNA decreased 67.6 and 87.4%, respectively, whereas Cyp1a2 mRNA increased 43.2%, and enzyme activities of Cyp3a11 and Cyp2d22 decreased 67.9 and 32.4%, respectively. Cyp2a4, 2b10 and Cyp2c29 were not altered at both mRNA and enzyme activity levels. Conclusions If studies in mice extrapolate to humans, lower doses of berberine appear to present a low risk of producing drug-drug interactions as a result of changed Cyp enzyme activity. However, high doses of berberine may suppress Cyp activities and result in drug-drug interactions.

show abstract

Effect of berberine on expression of hepatocyte nuclear factor-4α in rats with fructose-induced insulin resistance

Cited by 11 publications

References 8 publications

The Nociceptive and Anti-Inflammatory Effects ofArtemisia dracunculusL. Aqueous Extract on Fructose Fed Male Rats

The Nociceptive and Anti-Inflammatory Effects ofArtemisia dracunculusL. Aqueous Extract on Fructose Fed Male Rats

The Characterization of High‐Fat Diet and Multiple Low‐Dose Streptozotocin Induced Type 2 Diabetes Rat Model

Dose–response of berberine on hepatic cytochromes P450 mRNA expression and activities in mice

Contact Info

Product

Resources

About