Effect of Bcl-2-siRNA on proliferation and apoptosis of pediatric acute B lymphoblastic leukemia (A-BLL) cells

Meng, Weizhen; Liu, J P; Wang, X W

doi:10.4238/2015.october.16.9

Cited by 4 publications

(4 citation statements)

References 27 publications

(24 reference statements)

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“…The findings of the present study are consistent with previous research on leukemia. The therapeutic method of this study using siRNA could significantly downregulate Mcl-1 mRNA in Ju- On the other hand, another study by Meng et al on pediatric patients with A-BLL demonstrated that transfection of Bcl-2-siRNA into leukemic cells significantly increased the apoptosis rate (36).…”

Section: Discussionmentioning

confidence: 59%

Myeloid Cell Leukemia-1 (MCL-1) siRNA Therapy Showed Cytotoxic Effect on T Cells Acute Lymphoblastic Leukemia

et al. 2019

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Background: T-lineage acute lymphoblastic leukemia (T-ALL) is a malignant hyperplastic disease of the hematopoietic system. This tumor is the most common tumor in children and adolescents. Myeloid cell leukemia-1 (Mcl-1) is described as a prosurvival protein from the Bcl2 family. It is an important factor in routine cancer treatments. In fact, in different types of cancers, Mcl-1 downregulation can be a potential target. Objectives: The present study aims to evaluate the cytotoxic effect of MCL-1 siRNA in TALL cells. Methods: The present study evaluated the effects of Mcl-1 small interfering RNAs (siRNAs) on survival in Jurkat cells. Specific Mcl-1 siRNA was transfected and using quantitative real-time PCR, the relative expression of Mcl-1 mRNA was determined. Moreover, cell survival was determined using the colorimetric MTT assay. Results: The expression of mRNA reduced effectively in a dose-dependent manner at 48 hours after transfection with Mcl-1 siRNA. In addition, Mcl-1 siRNA treatment could significantly reduce tumor cell survival. Conclusions: Based on the results, downregulation of Mcl-1 by specific siRNAs in TALL cells can effectively reduce cell survival. Therefore, Mcl-1 siRNA may be a complementary agent along with standard methods in the treatment of TALL .

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Section: Discussionmentioning

confidence: 59%

Myeloid Cell Leukemia-1 (MCL-1) siRNA Therapy Showed Cytotoxic Effect on T Cells Acute Lymphoblastic Leukemia

et al. 2019

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“…Recent evidence also shows that selective targeting of these pathways has cytotoxic effects on cancer stem cells (See Table 2). (Bolomsky et al, 2020) Bcl family proteins/gene silencing (i) Bcl-2 siRNA and antisense nucleotides (Gagliardi and Ashizawa, 2022;Meng et al, 2015) (ii) Bim-1 siRNA (Schwulst et al, 2008;Wakabayashi et al, 2021) Targeting p53…”

Section: Drugs Targeting Autophagymentioning

confidence: 99%

Apoptosis and Autophagy: Therapeutic Implications in Cancer

Halder

2024

IJERR

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Despite the advances in the medical field so far, cancer remains a global health priority even now. Considering the drug resistance and the failure of cancer therapies to achieve complete eradication of cancer cells in certain populations, developing molecules that induce programmed cell death or apoptosis has been the focus of cancer research for several decades. Apoptosis evasion is one of the hallmarks of cancer cells, and efforts continue to achieve complete annihilation of cancer cells through selective killing. On the other hand, autophagy, a mode of cell degradation, is considered a double-edged sword. Recent studies show that autophagy also can be manipulated to selectively target cancer cells based on the tumor microenvironment and cellular context. Studies show that autophagy is an evolutionarily conserved process initiated during stress response and has enormous importance in maintaining physiological balance. Most importantly, the dynamic equilibrium between apoptosis and autophagy is crucial in maintaining cellular homeostasis. Although a ‘cell eating’ process, the fate of autophagic cells depends entirely on the nature of stress and the extent of crosstalk between autophagy. This understanding is of immense significance when designing therapeutic interventions targeting apoptosis and autophagy. Currently, several studies are ongoing to gain insights into the role of autophagy in cancer initiation, invasion, progression, angiogenesis, and metastasis. This review focuses on the two major cell death mechanisms, apoptosis and autophagy, in the context of cancer, their crosstalk, and the therapeutic interventions targeting both modes of cell death.

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“…Meng et al 21 indicated that the paediatric ALL patients in the drug-resistance group showed significantly higher Bcl-2 mRNA expression, compared to those in the chemotherapy sensitive group. In addition, the Bcl-2 mRNA expression in chemo-sensitive patients decreased significantly after chemotherapy, while the expression of Bcl-2 mRNA in the drug-resistant group did not alter significantly before and after chemotherapy.…”

mentioning

confidence: 99%