Effect of basic fibroblast growth factor on cartilage regeneration in chondrocyte-seeded collagen sponge scaffold

Fujisato, Toshiya; Sajiki, Toshinobu; Liu, Qiang; Ikada, Yoshito

doi:10.1016/0142-9612(96)85760-7

Cited by 176 publications

(84 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Basic FGF secreted by fibroblasts, platelets or other cells is responsible for connective tissue formation and wound healing [4,20]. Recently, bFGF was demonstrated to regenerate damaged articular cartilage in the mouse OA model [8,11].…”

Section: Discussionmentioning

confidence: 99%

Correlation between plasma and synovial fluid basic fibroblast growth factor with radiographic severity in primary knee osteoarthritis

Honsawek

Yuktanandana

Tanavalee

et al. 2011

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

Purpose The aim of this study was to investigate plasma and synovial fluid basic fibroblast growth factor (bFGF) levels in patients with primary knee osteoarthritis (OA) and to evaluate the correlation between bFGF levels and disease severity. Methods Thirty-five patients with knee OA and 15 healthy individuals were recruited into this study. Knee OA grading was performed according to the Kellgren-Lawrence classification. bFGF concentrations in both plasma and synovial fluid were determined using enzyme-linked immunosorbent assay. Results Plasma and synovial fluid bFGF levels in knee OA patients were significantly higher than in controls (P<0.001). Moreover, plasma and synovial fluid bFGF concentrations were positively correlated with radiographic severity (r0 0.535, P<0.001 and r00.570, P<0.001, respectively). Further analysis revealed that there was a positive correlation between plasma and synovial fluid bFGF levels (r00.674, P<0.001). Conclusions Plasma and synovial fluid bFGF levels were significantly increased in OA patients, and these elevated levels were positively correlated with radiographic severity.These findings indicate that bFGF levels may be a monitor of disease severity and could play an essential part in the pathophysiology of degenerative process in OA.

show abstract

Section: Discussionmentioning

confidence: 99%

Correlation between plasma and synovial fluid basic fibroblast growth factor with radiographic severity in primary knee osteoarthritis

Honsawek

Yuktanandana

Tanavalee

et al. 2011

International Orthopaedics (SICOT)

View full text Add to dashboard Cite

show abstract

“…A thin and continuous fibrous capsule also surrounded both membranes, which is consistent with other studies evaluating collagen or HyA biomaterials implanted at this location. [52][53][54][55][56] Using the fibrous capsule as a reference point for the membrane edges, the approximate thickness of membranes with and without BMP-2 after implantation was found to be similar. However, membranes without BMP-2 contained multiple voids within its structure, whereas membranes with BMP-2 showed a dense structure in which the membrane seemed to be completely filled with cells and the extracellular matrix (Fig.…”

Section: Bmp-2-releasing Self-assembled Membranesmentioning

confidence: 99%

Osteogenic Potential of BMP-2-Releasing Self-Assembled Membranes

Chung

Chien

Aguado

et al. 2013

Tissue Engineering Part A

View full text Add to dashboard Cite

We report here the use of novel self-assembling collagen-hyaluronic acid (HyA) membranes to deliver bone morphogenetic protein-2 (BMP-2) for orthopedic applications. Prior work has demonstrated that collagen-HyA membranes are formed initially through electrostatic interactions between the oppositely charged collagen and HyA molecules, and that membrane growth is driven by osmotic pressure imbalances between the collagen and HyA solutions. The purpose of this study was to investigate the potential of incorporating charged growth factors such as BMP-2 within the membrane for regenerative medicine applications. Membrane material properties, protein mass loss, and release kinetics of BMP-2, as well as biocompatibility and osteogenic potential in vitro and in vivo using a subcutaneous mouse model were assessed. Scanning electron microscopy and mechanical testing confirmed no loss of structural or mechanical integrity upon BMP-2 incorporation into the membranes. Slow and steady release of the growth factor was demonstrated with 17% of total loaded BMP-2 released over the course of 49 days. To test biocompatibility and osteogenic potential in vitro, human mesenchymal stem cells were cultured on collagen-HyA membranes and showed greater proliferation rates (for up to 28 days) on membranes without BMP-2, but a greater alkaline phosphatase activity and osteocalcin production on membranes releasing BMP-2. In vivo subcutaneous implantation of the membranes showed a minimal immune response with osteoblasts and mineral deposits present in the ectopic site for BMP-2-releasing membranes, further demonstrating the potential of the BMP-2-releasing membranes to induce osteogenic differentiation. This study presents a novel strategy to create self-assembled membranes using two biocompatible molecules that can deliver bioactive agents in a sustained manner to induce a local regenerative response.

show abstract

“…Surgical techniques such as a discectomy, arthroplasty, and condylectomy have been used to provide migration of blood cells and mesenchymal stem cells from the bone marrow to the defect [4]. These surgical treatments achieve fibrocartilagenous healing that may improve symptoms; however, the reparative tissues often cause limitations of joint function [5].…”

Section: Introductionmentioning

confidence: 99%

Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template

Wang¹,

Chan²,

Chiu³

et al. 2017

Preprint

View full text Add to dashboard Cite

Previous study demonstrated the reconstituted type I collagen matrix extracted from rabbit tendons enabled to regenerate the TMJ disc in the rabbit. The aim of this study was to investigate changes in the extracellular matrix (ECM) and mechanisms of regeneration in TMJ disc. In 36 New Zealand rabbits that underwent a partial discectomy, discs were replaced with reconstituted collagen templates for 3 months.A histological analysis showed that moderate to severe degeneration appeared in partially discectomized joints without implantation. In contrast, discs that received the reconstituted collagen template regenerated, and returned to normal to protect the joint. Cells in the regenerative tissue expressed ECM, and fibers became regular and compact due to tissue remodeling over time. Reparative cells differentiated into chondroblasts, and showed highly dense pericellular fibers. The morphology and collagen composition of the disc and condyle in the 3-month experimental group were similar to those of normal tissues. In conclusion, the reconstituted collagen template facilitated the regeneration of surgically discectomized discs. Type I and type II collagens play a crucial role in the regeneration of articular discs.

show abstract

Effect of basic fibroblast growth factor on cartilage regeneration in chondrocyte-seeded collagen sponge scaffold

Cited by 176 publications

References 23 publications

Correlation between plasma and synovial fluid basic fibroblast growth factor with radiographic severity in primary knee osteoarthritis

Correlation between plasma and synovial fluid basic fibroblast growth factor with radiographic severity in primary knee osteoarthritis

Osteogenic Potential of BMP-2-Releasing Self-Assembled Membranes

Histological and Immunohistochemical Analyses of Repair of the Disc in the Rabbit Temporomandibular Joint Using a Collagen Template

Contact Info

Product

Resources

About