Effect of Basic Fibroblast Growth Factor; An In Vitro Study of Tendon Healing

Chan, Barbara Pui; Chan, Kai-Ming; Maffulli, Nicola; Webb, Sarah; Lee, Kenneth K.

doi:10.1097/00003086-199709000-00031

Cited by 137 publications

(98 citation statements)

References 25 publications

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“…The stimulation of ACL cell proliferation by FGF-2 is consistent with previous reports [6,29,41]. The combination of cell proliferation and slowing of scaffold dimensional change may be useful for applications where it is desirable for the scaffold to maintain its shape during remodeling.…”

Section: Discussionsupporting

confidence: 89%

The effect of selected growth factors on human anterior cruciate ligament cell interactions with a three‐dimensional collagen‐GAG scaffold

Murray

Rice

Wright

et al. 2003

Journal Orthopaedic Research

124

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Our work focuses on development of a collagen-glycosamimoglycan (CC) scaffold to facilitate ligament healing in the gap between the ruptured ends of the human anterior cruciate ligament (ACL). In the present investigation, we evaluated the effects of selected growth factors on human ACL cell responses important in tissue regeneration, namely cell migration, proliferation, collagen production, and expression of a-smooth muscle actin (SMA).Methods: Explants from six human ACLs were cultured on top of a CG scaffold. Culture conditions were with either 2% FBS (control), or 2% FBS supplemented with TGF-01, PDGF-AB, EGF, or FGF-2. Histologic cell distribution, total DNA content, proliferation rate, rate of collagen synthesis, scaffold diameter and percentage of SMA positive cells were determined at two, three and four weeks.Results: The addition of TGF-01 to the culture medium resulted in increased cell number, increased collagen production and increased expression of SMA within the scaffold. Supplementation with PDGF-AB resulted in increased cell proliferation rates within the scaffold and increased collagen production. The addition of FGF-2 resulted in increased cell proliferation rates and slowed rates of scaffold shrinkage when compared with the control group.Discussion: This work suggests that certain growth factors can alter the biologic functions of human ACL cells in a CG scaffold implanted as a bridge at the site of an ACL rupture. Based on these findings, the addition of selected growth factors to an implantable CG scaffold may facilitate ligament healing in the gap between the ruptured ends of the human ACL.

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Section: Discussionsupporting

confidence: 89%

The effect of selected growth factors on human anterior cruciate ligament cell interactions with a three‐dimensional collagen‐GAG scaffold

Murray

Rice

Wright

et al. 2003

Journal Orthopaedic Research

124

View full text Add to dashboard Cite

show abstract

“…The number of cells that migrated to the lower surface of the lter, per mm 2 , was counted 6 times on each lter using a 1-mm 2 eyepiece grid, and the average was determined. The chemotactic activity of cells was normalized by the control well in which only culture medium was added to the lower chamber, in accord with a previous report (Chan et al 1997), where chemotactic activity (%) = number of cells per mm 2 in presence of growth factors/number of cells per mm 2 in control well ´ 100 (%). Data were expressed as mean chemotactic activity (%) with standard deviation.…”

Section: Chemokinesis Assaymentioning

confidence: 88%

Effects of hyaluronic acid and basic fibroblast growth factor on motility of chondrocytes and synovial cells in culture

Maniwa

Ochi

Motomura

et al. 2001

Acta Orthopaedica Scandinavica

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“…This special orientation of the cells reflects their function. These cells are capable of synthesizing fibrillose as well as non-fibrillar components of the extracellular matrix and can also resorb collagen fibers (Chan et al, 1997;Gerich et al, 1996). The fascicles are covered with epitenon, which in turn is covered with paratenon.…”

Section: Tendon Structurementioning

confidence: 99%