2014
DOI: 10.2141/jpsa.0130095
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Effect of Bacteriophage on the Transcriptional and Translational Expression of Inflammatory Mediators in Chicken Macrophage

Abstract: This study was designed to evaluate the effect of bacteriophage on macrophage-mediated inflammatory immune responses against intracellular pathogens. The intracellular survival of nonlysogenic, lysogenic Salmonella Typhimurium, and Listeria monocytogenes were evaluated in chicken macrophage HD11 cells treated with Salmonella bacteriophage P22 for 24 h at 37℃. The relative expression of inflammatory mediator-related genes (IL-1β, IL-6, IL-8, IL-10, LITAF, and iNOS) was estimated by using a qPCR. The production … Show more

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Cited by 4 publications
(4 citation statements)
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“…J1 bacteriophage was propagated in MRS broth containing host strain LP at 37 °C under aerobic condition with 5% CO 2 for 48 h. The culture was centrifuged at 3000× g for 20 min and filtered through a 0.2 μm sterilized filter. The bacteriophages were further purified following method previously described [21].…”
Section: Methodsmentioning
confidence: 99%
“…J1 bacteriophage was propagated in MRS broth containing host strain LP at 37 °C under aerobic condition with 5% CO 2 for 48 h. The culture was centrifuged at 3000× g for 20 min and filtered through a 0.2 μm sterilized filter. The bacteriophages were further purified following method previously described [21].…”
Section: Methodsmentioning
confidence: 99%
“…For expression of S. aureus virulence genes, cells were infected and treated in a similar fashion to the association and invasion assay, but scaled up to 6 ml in 25 cm 2 flasks. RNA was extracted using methods described in Ahn et al (2014), and a NanoDrop ND-1000 was used to measure the concentration of the extracted RNA. One microgram of RNA was used to create cDNA using the instructions provided in the Verso cDNA Synthesis kit (Thermo Scientific).…”
Section: Methodsmentioning
confidence: 99%
“…One microgram of RNA was used to create cDNA using the instructions provided in the Verso cDNA Synthesis kit (Thermo Scientific). All quantitative PCR used the methods described in Ahn et al (2014) and Salaheen et al (2014b), except that cDNA was diluted 10-fold. The virulence genes measured were agrA, sodA, sirA, hla, hlb, spa, sbi, sae, sarA, sarR, sarS and sigB, and the reference gene used was the 16S rRNA gene.…”
Section: Methodsmentioning
confidence: 99%
“…Among these pathogens, EHEC, Salmonella, and Listeria have drawn the most attention (CDC, 2013;Cummings et al, 2012;Dussurget, 2008;Teunis, Ogden, & Strachan, 2008). The human-enteric bacterial pathogens interaction and their infection process is usually initiated by intestinal epithelial cell adhesion and following by cell invasion through site-specific ligands (Ahn, Kim, Jung, & Biswas, 2014). Infections with these foodborne enteric pathogens and their severity are also highly influenced by normal gut microbiota, and the immunity of the host.…”
Section: Introductionmentioning
confidence: 99%