Effect of bacterial inoculum ratio in mixed culture for decolourization and detoxification of pulp paper mill effluent

Chandra, Ram; Singh, Rachna; Yadav, Sangeeta

doi:10.1002/jctb.2758

Cited by 32 publications

(20 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The chromosome aberrations also revealed that pulp and paper mill effluent contained many androgenic compounds that induced these aberrations during the cell division. Similar aberrations have been reported by Chandra et al (2012) and Chandra and Singh (2012). …”

Section: Discussionsupporting

confidence: 90%

“…Similarly, comparison of HPLC chromatograms of the control and degraded sample at 250 and 320 nm revealed an apparent reduction of peaks at periodic bacterial incubation times, which also gave strong evidence for the disappearance of organic pollutants present in the effluent. Similar HPLC data have been recorded in our studies of degradation of ligninocellulogic compounds present in pulp and paper mill waste (Chandra and Singh, 2012; Chandra et al, 2012; Sangeeta and Chandra, 2015). Detection of 2-butoxyethanol in degraded effluent after 6 days of biostimulation revealed that the detected compounds were likely generated as metabolic products from lignino-organic complexes present in the effluent.…”

Section: Discussionsupporting

confidence: 88%

See 1 more Smart Citation

RETRACTED: Biodegradation of Endocrine-Disrupting Chemicals and Residual Organic Pollutants of Pulp and Paper Mill Effluent by Biostimulation

Chandra¹,

Sharma²,

Yadav³

et al. 2018

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Effluent discharged from the pulp and paper industry contains various refractory and androgenic compounds, even after secondary treatment by activated processes. Detailed knowledge is not yet available regarding the properties of organic pollutants and methods for their bioremediation. This study focused on detecting residual organic pollutants of pulp and paper mill effluent after biological treatment and assessing their degradability by biostimulation. The major compounds identified in the effluent were 2,3,6-trimethylphenol, 2-methoxyphenol (guaiacol), 2,6-dimethoxyphenol (syringol), methoxycinnamic acid, pentadecane, octadecanoic acid, trimethylsilyl ester, cyclotetracosane, 5,8-dimethoxy-6-methyl-2,4-bis(phenylmethyl)napthalen-1-ol, and 1,2-benzendicarboxylic acid diisononyl ester. Most of these compounds are classified as endocrine-disrupting chemicals and environmental toxicants. Some compounds are lignin monomers that are metabolic products from secondary treatment of the discharged effluent. This indicated that the existing industrial process could not further degrade the effluent. Supplementation by carbon (glucose 1.0%) and nitrogen (peptone 0.5%) bio-stimulated the degradation process. The degraded sample after biostimulation showed either disappearance or generation of metabolic products under optimized conditions, i.e., a stirring rate of 150 rpm and temperature of 37 ± 1°C after 3 and 6 days of bacterial incubation. Isolated potential autochthonous bacteria were identified as Klebsiella pneumoniae IITRCP04 (KU715839), Enterobacter cloacae strain IITRCP11 (KU715840), Enterobacter cloacae IITRCP14 (KU715841), and Acinetobacter pittii strain IITRCP19 (KU715842). Lactic acid, benzoic acid, and vanillin, resulting from residual chlorolignin compounds, were generated as potential value-added products during the detoxification of effluent in the biostimulation process, supporting the commercial importance of this process.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 88%

RETRACTED: Biodegradation of Endocrine-Disrupting Chemicals and Residual Organic Pollutants of Pulp and Paper Mill Effluent by Biostimulation

Chandra¹,

Sharma²,

Yadav³

et al. 2018

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Optimum production of laccase from strains of Serratia marcescens has been reported in mesophilic temperature range with neutral to alkaline pH (Chandra et al 2011; Verma and Madamwar 2003). Serratia marcescens , in the present study, exhibited the peculiarity with respect to its ability to produce laccase in a wider temperature (5–45°C) and pH (5–13) range.…”

Section: Discussionmentioning

confidence: 99%

A psychrotolerant strain of Serratia marcescens (MTCC 4822) produces laccase at wide temperature and pH range

2015

View full text Add to dashboard Cite

A psychrotolerant bacterial strain of Serratia marcescens, originally isolated from a glacial site in Indian Himalayan Region (IHR), has been investigated for laccase production under different culture conditions. The bacterial strain was found to grow between 4 to 45°C (opt. 25°C) and 3 to 14 pH (opt. 5 pH) on prescribed growth medium, coinciding with production of laccase in laccase producing medium. However, the production of laccase was more consistent toward alkaline pH. Laccase enzyme was partially purified using gel filtration chromatography. The molecular mass of laccase was determined ~53 kDa on native PAGE. The Km and Vmax values were determined to be 0.10 mM and 50.00 μM min−1, respectively, with ABTS. Inoculum size (4.0% v/v at 1.5 O.D.) resulted in significantly higher production of laccase. Carbon and nitrogen sources also affected the laccase production significantly. All the carbon sources enhanced laccase production, xylose being the best enhancer (P < 0.01). Among nitrogen sources, organic sources were found to act as inhibitors (P < 0.01), and among the in-organic sources only sodium nitrate enhanced the laccase production. Low molecular weight organic solvents significantly (P < 0.01) enhanced laccase production up to 24 h of incubation with a decline in later incubation period. Production of laccase by the psychrotolerant bacterium in wide range of temperature and pH is likely to have inference in biotechnological processes.

show abstract

“…Likewise, the sul ide is needed to hasten the cleavage of phenolic units by attacking the ether links. The destruction of the carbon-oxygen bond is one of the most important reactions in the chemical pulping process [19]. The 18% active alkali gives the lowest bamboo Kappa number [18], while the sul idity value of 15% results to the optimum deligni ication rate [20].…”

Section: B Production Of Cnfmentioning

confidence: 99%

Mechanical Production of Cellulose Nanofibrils (CNF) from bleached kawayan tinik (Bambusa blumeana Schult. F) pulp: Effect of holocellulose preparation and number of passes in the supermass colloider

Silos¹,

Razal²

2019

J. appl. phys. sci.

View full text Add to dashboard Cite

The study determined the effects of holocellulose preparation and number of passes in the mechanical production of CNF from bleached Kawayan Tinik pulp. Pretreatment of bamboo included removal of branches and outer skin, cutting into rings, chipping, soaking in water, air-drying, and hammer milling. The Kraft Pulping and the onestep hypochlorite bleaching were used for pulp liberation and bleaching, respectively. A portion of the bleached pulp underwent the sodium chlorite treatment to isolate the holocellulose by removing extractives, residual lignin, and some hemicelluloses. The amount of acid-insoluble lignin present in the raw bamboo, Kraft pulp, bleached pulp, and holocellulose was determined. The bleached pulp and holocellulose were separately made to pass for a predetermined number of cycles in the supermass colloider to produce CNF. The yields were calculated, with ultimate values of 73.52% (200 passes) and 66.02% (300 passes) for the bleached pulp and holocellulose preparation, respectively. Optical microscopy was done to monitor the changes in the morphological characteristics of the CNF during the initial passes, while Scanning Electron Microscopy showed the nanosize dimensions of the inal product with an average diameter of 58.35 nm and an average length of 2,169.26 nm. Dynamic Light Scattering (DLS) showed a homogeneous particle size distribution. In addition, the functional groups present in the CNF from bleached pulp and holocellulose were analyzed using Fourier Transform Infrared (FTIR) Spectroscopy and showed that the CNF samples contain peaks for O-H, C-H, and C-O-C stretching, but lacks groups related to lignin. X-ray Diffraction Analysis showed that the crystallinity of the CNF increased to 71% compared to the 60% from literature. Statistical analysis showed that holocellulose preparation and number of passes in the supermass colloider had signi icant effects on the CNF yield, length, width, and aspect ratio. The holocellulose which was made to pass the colloider for 200 times gave the highest yield and the morphological characteristics closest to reported literature values. For bleached pulps, the number of passes in the supermass colloider had signi icant effect on the CNF length and width.

show abstract

Effect of bacterial inoculum ratio in mixed culture for decolourization and detoxification of pulp paper mill effluent

Cited by 32 publications

References 29 publications

RETRACTED: Biodegradation of Endocrine-Disrupting Chemicals and Residual Organic Pollutants of Pulp and Paper Mill Effluent by Biostimulation

RETRACTED: Biodegradation of Endocrine-Disrupting Chemicals and Residual Organic Pollutants of Pulp and Paper Mill Effluent by Biostimulation

A psychrotolerant strain of Serratia marcescens (MTCC 4822) produces laccase at wide temperature and pH range

Mechanical Production of Cellulose Nanofibrils (CNF) from bleached kawayan tinik (Bambusa blumeana Schult. F) pulp: Effect of holocellulose preparation and number of passes in the supermass colloider

Contact Info

Product

Resources

About