Effect of bacterial infection on antioxidant activity and lipid peroxidation in the midgut of Galleria mellonella L. larvae (Lepidoptera, Pyralidae)

Dubovskiy, Ivan M.; Martemyanov, V. V.; Vorontsova, Yana; Rantala, Markus J.; Gryzanova, E.V.; Глупов, В. В.

doi:10.1016/j.cbpc.2008.02.003

Cited by 167 publications

(149 citation statements)

References 39 publications

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“…An enhanced level of MDA (lipid peroxidation product) in the midgut of larvae was observed in response to Nosema infection (Fig-1A). Similar to this higher level of LPX was also detected in insects during viral (Wang et al, 2001) and bacterial infection in insects (Dubovskiy et al, 2008). It is understood that one of the most immediate immune response of the gut involves the production of ROS to fight microbial infection both in mammals (Kinnula et al, 1992;Geiszt et al, 2003) and insects (Ha et al, 2005a,b;Ryu et al, 2010).…”

Section: Discussionsupporting

confidence: 59%

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Oxidative Stress and Antioxidant Defences in the Tasar Silkworm Antheraea mylitta D: Challenged with Nosema Species

Jena¹,

Pandey²,

Sinha³

2014

International Journal of Industrial Entomology

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This study was designed to find out the effect of Nosema spore on oxidative damages and antioxidant defence in the midgut of tasar silkworm Antheraea mylitta. Higher level of lipid peroxidation (LPX) and total hydroperoxides indicate the resultant oxidative stress in the Nosema exposed specimen. Increased superoxide dismutase (SOD) suggests activation of physiological mechanism to scavenge the superoxide radical produced during Nosema infection. Higher activities of catalase and glutathione-S-tranferase on 18 th d indicate adaptive behaviour of the tissue against oxyradicals. The results suggest that Nosema infection is involved in altering the active oxygen metabolism by modulating LPX and reactive oxygen species (ROS), which is indicative of pebrine disease disorder. As ingestion is the main entry route of many pathogens, the intestinal epithelium is the first line of defense against invasion and dissemination of pathogenic microorganisms.In this view insect innate immune system plays a vital role in the defense against microorganisms (Medzhitov and Janeway, 1997). One of the most immediate epithelial responses in mammals to combat the pathogen is the generation of antimicrobial reactive oxygen species (Cohn et al., 1994;Geiszt et al., 2003). It is to be noted that ROS can affect both entomopathogens and the host tissues, and may lead to damages of cellular biomolecules such as lipids, proteins and nucleic acids.To protect against these toxic cellular environment, insect possess a suit of antioxidant defence mechanisms, comprised of both enzymatic as well as non-enzymatic components.

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Section: Discussionsupporting

confidence: 59%

“…Similarly, higher SOD activity was also observed in midgut of insect (Galleria mellonella) infection with bacterial pathogen (Dubovskiy et al, 2008). The induction of SOD during Nosema infection may indicate that it helps in inhibiting the oxygen radical accumulation.…”

Section: Determination Of Antioxidant Status Of Larva Exposed Tomentioning

confidence: 84%

Oxidative Stress and Antioxidant Defences in the Tasar Silkworm Antheraea mylitta D: Challenged with Nosema Species

Jena¹,

Pandey²,

Sinha³

2014

International Journal of Industrial Entomology

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“…The homogenates were centrifuged for 5 min, ×10,000 g at 4°C. The supernatants were used immediately to estimate the activity of glutathione-S-transferase (GST), superoxide dismutase (SOD), lipid peroxidase and esterase (EST) according to the method described by Dubovskiy et al [61]. …”

Section: Methodsmentioning

confidence: 99%

Highly specific host-pathogen interactions influence Metarhizium brunneum blastospore virulence against Culex quinquefasciatus larvae

et al. 2018

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Entomopathogenic fungi are potential biological control agents of mosquitoes. Our group observed that not all mosquitoes were equally susceptible to fungal infection and observed significant differences in virulence of different spore types. Conidiospores and blastospores were tested against Culex quinquefasciatus larvae. Blastospores are normally considered more virulent than conidia as they form germ tubes and penetrate the host integument more rapidly than conidia. However, when tested against Cx. quinquefasciatus, blastospores were less virulent than conidia. This host-fungus interaction was studied by optical, electron and atomic force microscopy (AFM). Furthermore, host immune responses and specific gene expression were investigated. Metarhizium brunneum (formerly M. anisopliae) ARSEF 4556 blastospores did not readily adhere to Culex larval integument and the main route of infection was through the gut. Adhesion forces between blastospores and Culex cuticle were significantly lower than for other insects. Larvae challenged with blastospores showed enhanced immune responses, with increased levels of phenoloxidase, glutathione-S-transferase, esterase, superoxide dismutase and lipid peroxidase activity. Interestingly, M. brunneum pathogenicity/stress-related genes were all down-regulated in blastospores exposed to Culex. Conversely, when conidia were exposed to Culex, the pathogenicity genes involved in adhesion or cuticle degradation were up-regulated. Delayed host mortality following blastospore infection of Culex was probably due to lower adhesion rates of blastospores to the cuticle and enhanced host immune responses deployed to counter infection. The results here show that subtle differences in host-pathogen interactions can be responsible for significant changes in virulence when comparing mosquito species, having important consequences for biological control strategies and the understanding of pathogenicity processes.

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“…Homojenatlar +4 o C'de 10.000 rpm'de 30 dakika süreyle santrifüj edilmiş ve elde edilen süpernatantlarda CAT ve SOD aktiviteleri ile GSH, MDA ve protein düzeyleri spektrofotometrik yöntemlerle belirlenmiştir. CAT aktivitesi [13]; SOD aktivitesi [14]; GSH düzeyi [15]; MDA düzeyi [16], ve total protein miktarı ise [17] Thiamethoxam etkileşimine yanıtta O. niloticus'ta incelenen oksidatif stres parametrelerinde dokuya, ortam derişimi ve etki süresine bağlı olarak önemli değişimlerin meydana geldiği belirlenmiştir. Araştırma sonuçlarımızla benzer olarak daha önceki çalışmalarda da balıkların dokularındaki antioksidan savunma sistemlerinde meydana gelen değişimlerin çalışılan pestisitlere ve bu kimyasalların etki süreleri ile ortam derişimlerine bağlı olarak oluştuğu rapor edilmiştir [19,20].…”

Section: Materyal Ve Yöntemunclassified

Neonikotinoid insektisit thiamethoxamın Oreochromis niloticus’ta oksidatif stres parametreleri üzerine etkisi

Fırat¹,

Aytekin²

2018

Balıkesir Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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Effect of bacterial infection on antioxidant activity and lipid peroxidation in the midgut of Galleria mellonella L. larvae (Lepidoptera, Pyralidae)

Cited by 167 publications

References 39 publications

Oxidative Stress and Antioxidant Defences in the Tasar Silkworm Antheraea mylitta D: Challenged with Nosema Species

Oxidative Stress and Antioxidant Defences in the Tasar Silkworm Antheraea mylitta D: Challenged with Nosema Species

Highly specific host-pathogen interactions influence Metarhizium brunneum blastospore virulence against Culex quinquefasciatus larvae

Neonikotinoid insektisit thiamethoxamın Oreochromis niloticus’ta oksidatif stres parametreleri üzerine etkisi

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