Effect of Bacoside Extract fromBacopa monnieraon Physical Fatigue Induced by Forced Swimming

Abstract

The antifatigue effect of bacoside extract (BME) from Bacopa monniera (L.) Wettst. was investigated. Rats were subjected to weight-loaded forced swim test (WFST) every alternate day for 3 weeks. The BME at a dosage of 10 mg/kg body weight was administered orally to rats for 2 weeks in order to evaluate the following biomarkers of physical fatigue: swimming time, change in body weight, lipid peroxidation, lactic acid (LA), glycogen, antioxidant enzyme activities such as superoxide dismutase (SOD) and catalase (CAT) and blood parameters, namely blood urea nitrogen (BUN) and creatine kinase (CK). The exhaustive swimming time was increased by 3-fold in the BME supplemented group compared with that of the control group on day 13. The BME treatment lowered malondialdehyde (MDA) levels in brain, liver and muscle tissues by 11.2%, 16.2% and 37.7%, respectively, compared with the control exercised group (p < 0.05). The BME also reduced the LA, serum BUN and CK activities significantly compared with that of the control. Administration of BME significantly protected the depletion of SOD and CAT activities. The HSP-70 expression studies by western blot also confirmed the antifatigue property of BME. The present study thus indicates that BME ameliorates the various impairments associated with physical fatigue.

Development of bacoside enriched date syrup juice and its evaluation for physical endurance

“…Antioxidants may be involved in glycogen metabolism so as to meet the energy requirements of working skeletal muscles and may act by terminating the chain reaction of lipid peroxidation so as to maintain the morphological stability of mitochondria in spinal motor neurons (Yu et al 2006). In our previous study, we have clearly shown that bacoside rich extract reduced the exercise-induced oxidative damage by inhibiting lipid peroxidation and enhancing the antioxidant defense by elevating the activities of antioxidant enzymes (Anand et al 2012). Effect of BRJ on lipid peroxidation, glycogen and lactic acid levels Swimming exercise significantly increased MDA level in muscle, liver and brain in control group compared to that of sedentary group.…”

“…In our earlier studies we have demonstrated the physical endurance capacity of bacoside rich extract in rats (Anand et al 2012). Since, the bacoside rich extract is bitter in taste.…”

“…Bacoside A is mainly working on the enhancement of cognitive performance. In our earlier studies we have reported that bacoside rich extract with antioxidants enhances the physical endurance (Anand et al 2012). Further studies is warranted to prove physical endurance of bacoside A mixture alone.…”

“…Bacoside rich extract is found to enhance physical endurance by protecting mitochondria, muscle fibers from the oxidative damage. It also improves enzymatic and nonenzymatic antioxidant status (Anand et al 2012). This antioxidant property of bacoside with added energy and minerals improves physical performance.…”

“…(Singh and Dhawan 1992). These active principles, apart from facilitating learning and memory in normal rats, it has several other health benefits such as anti-stress (Chowdhuri et al 2002), anti-inflammatory (Channa et al 2006), anxiolytic (Ernst 2006), vasodilator (Channa et al 2003) and antifatigue properties (Anand et al 2012).…”