Effect of Bacillus subtilis on Aeromonas hydrophila infection resistance in juvenile freshwater prawn, Macrobrachium rosenbergii (de Man)

Keysami, Mehran Avakh; Mohammadpour, Masoumeh

doi:10.1007/s10499-012-9588-3

Cited by 22 publications

(8 citation statements)

References 21 publications

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“…In aquaculture, probiotics have captured special attention for its efficacious function for supplementary synthesis of digestive enzymes [20], and improvement in growth performance of the farmed species [21,22]. Bacillus have been widely used as putative probiotics for its beneficial nutritional impact, antagonism against microbial pathogens and easy supplementation [23,24], as reported for larval development of Gilthead sea bream (Sparus aurata, L.) by Arıg et al [25].…”

Section: Resultsmentioning

confidence: 99%

Dietary Administered Bacillus sp. PP9 Enhances Growth, Nutrition and Immunity in Cirrhinus mrigala (Hamilton)

Bandyopadhyay

Sarkar

Mahanty

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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Bacillus sp. PP9, a noble bacterial isolate of mrigal (Cirrhinus mrigala) gut was investigated for its efficacy as a dietary probiotic against mrigal (C. mrigala) through a dose dependent impact assessment. Mrigal fingerlings (avg.wt. 2.5 ± 0.20 g) were fed with three different doses (2 9 10 4 , 2 9 10 5 and 2 9 10 6 CFU) of Bacillus sp. PP9 admixed with 100 g feed for a period of 60 days. It was found that the feed with Bacillus concentration of 2 9 10 4 CFU exhibited significantly higher growth, maximum RNA DNA ratio, lower food conversion ratio in comparison to other two feed types. Enhanced intestinal protease and a-amylase activity followed by maximum hepatic glutamic oxaloacetic transaminase and glutamate pyruvate transaminase levels were also monitored in this specific feed type. Important hematological parameters like hemoglobin percentage, total erythrocyte count, total leukocyte count, corpuscular hemoglobin also indicated a healthy trend in this dose. Furthermore, the same dietary treatment exhibited highest levels of total serum protein, albumin globulin ratio and serum bactericidal activity. The growth, nutrition and immunological parameters showed a declining trend with increase in the bacterial concentration as well as in the control group (without probiotic). In summary, dietary supplement of 2 9 10 4 /100 g appears as a potential probiotic dose of Bacillus sp. PP9 for growth, nutritional efficacy as well as immunological modulation of C. mrigala and denotes a recommended concentration for its future field applications.

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Section: Resultsmentioning

confidence: 99%

Dietary Administered Bacillus sp. PP9 Enhances Growth, Nutrition and Immunity in Cirrhinus mrigala (Hamilton)

Bandyopadhyay

Sarkar

Mahanty

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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“…The A. hydrophila , AH‐1N (10 6 cells/ml), was first inoculated (day 0) via static immersion (Keysami & Mohammadpour, ) in the positive control and probiotic treatments. A second dose of bacterial challenge was performed on day 16 with an addition of AH‐1N (10 6 cells/ml) after the first inoculation.…”

Section: Methodsmentioning

confidence: 99%

“…The A. hydrophila, AH-1N (10 6 cells/ml), was first inoculated (day 0) via static immersion (Keysami & Mohammadpour, 2013) and muscle lipid peroxidation and measured as previously described.…”

Section: Experimental Challenge Of M Rosenbergii Juvenile With a mentioning

confidence: 99%

Dietary supplementation use of Bacillus cereus as quorum sensing degrader and their effects on growth performance and response of Malaysian giant river prawn Macrobrachium rosenbergii juvenile towards Aeromonas hydrophila

et al. 2018

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This study was conducted to investigate the effects of dietary probiotic quorum sensing degrader Bacillus cereus on the growth performance, intestinal short‐chain fatty acid levels, antioxidant status and histopathological response of Macrobrachium rosenbergii against Aeromonas hydrophila. M. rosenbergii juveniles were fed with 104 cfu/g probiotic B. cereus, while the control group was fed without B. cereus. After 28 days of feeding, growth performance was significantly higher (p < 0.05) in prawns fed with B. cereus compared to the control group. Probiotic‐fed prawns showed the highest propionic acid level, while no significant differences (p > 0.05) were observed for acetic acid or butyric acid levels. Dietary B. cereus supplementation significantly (p < 0.05) improved the superoxide dismutase (SOD) activity in the prawns. In the challenge study, no significant differences were observed between the treatments in survival and haemolymph SOD activity. However, histopathological observations of the hepatopancreas after A. hydrophila challenge showed hemocyte infiltrations and necrosis in the control prawns, while the probiotic‐fed prawns showed an overall better hepatopancreatic condition. These findings indicate that dietary B. cereus can be an effective growth promoter in M. rosenbergii and mitigates a compromise to the hepatopancreatic integrity during pathogenic challenge.

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“…Addition of Bacillus sp. as feed additives able to improve digestive activity of the prawn by synthesizing vitamins and cofactors resulting in greater growth performance (Keysami & Mohammadpour, 2013; Keysami et al., 2011) Therefore, the increase of SGR in prawn fed with the probiotic in this study might be due to stimulation of digestive enzymes.…”

Section: Discussionmentioning

confidence: 83%

“…Treatment feeds were prepared according to Allameh et al. (2015) and Keysami and Mohammadpour (2013) with slight modifications. Briefly, the pellets were soaked in a treatment solution (Table 1) for 30 min on a flat tray and oven‐dried at 32°C.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of Bacillus cereus strain BP‐MBRG/1b and prebiotic fructooligosaccharides dietary supplementation on growth performance and disease resistance of Macrobrachium rosenbergii (De Mann) towards Aeromonas hydrophila AH‐1N

Muthukrishnan

Hoong

Chen

et al. 2020

Aquaculture Research

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The effects of Bacillus cereus strain BP‐MBRG/1b (probiotic), 0.4% fructooligosaccharides (prebiotic) and its combination synbiotic dietary supplementations were evaluated in M. rosenbergii juveniles through feeding trials. After 28 days of feeding, the results showed that the probiotic fed juveniles had the highest specific growth rate (SGR) (1.00 ± 0.02%/day) compared with the prebiotic (0.42 ± 0.08%/day) and synbiotic (0.54 ± 0.08%/day) treatments. The prawns were then challenged with pathogenic Aeromonas hydrophila strain AH‐1N via static immersion. No significant differences were observed in terms of mortalities for the first challenge nor in the second challenge for all the treatments. Histological observations of the prawn hepatopancreas in all the treatment groups demonstrated necrosis and sloughing of the cells. Overall, the present result suggested that B. cereus strain BP‐MBRG/1b demonstrated enhancement of growth performance in M. rosenbergii compared with prebiotic and synbiotic treatments. However, further investigation is required to study the optimal dosage of the synbiotic treatment (probiotic B. cereus strain BP‐MBRG/1b and prebiotic fructooligosaccharides) against A. hydrophila challenged M. rosenbergii.

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Effect of Bacillus subtilis on Aeromonas hydrophila infection resistance in juvenile freshwater prawn, Macrobrachium rosenbergii (de Man)

Cited by 22 publications

References 21 publications

Dietary Administered Bacillus sp. PP9 Enhances Growth, Nutrition and Immunity in Cirrhinus mrigala (Hamilton)

Dietary Administered Bacillus sp. PP9 Enhances Growth, Nutrition and Immunity in Cirrhinus mrigala (Hamilton)

Dietary supplementation use of Bacillus cereus as quorum sensing degrader and their effects on growth performance and response of Malaysian giant river prawn Macrobrachium rosenbergii juvenile towards Aeromonas hydrophila

Efficacy of Bacillus cereus strain BP‐MBRG/1b and prebiotic fructooligosaccharides dietary supplementation on growth performance and disease resistance of Macrobrachium rosenbergii (De Mann) towards Aeromonas hydrophila AH‐1N

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