Effect of Bacillus subtilis and Signaling Molecules on the State of the Pro/Antioxidant System and the Expression of Protective Protein Genes in Potato Plants upon Phytophthorosis and a Moisture Deficit

Yarullina, L. G.; Tsvetkov, V. O.; Бурханова, Г. Ф.; Черепанова, Е. А.; Sorokan, A. V.; Zaikina, E. A.; Mardanshin, Ildar; Kalatskaya, J. N.; Balyuk, N. V.

doi:10.1134/s0003683821060132

Cited by 3 publications

(8 citation statements)

References 31 publications

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“…“Plant+endophyte” System: 15-day-old plants were inoculated according to the previously described method [ 35 ] with 5 mL of (1) water (control plants); (2) solutions of conjugates of chitosan with caffeic (ChCA, 0.30 mg/mL) or ferulic (ChFA, 0.25 mg/mL) acids; (3) suspensions of bacteria B. subtilis strains 26D or 11 VM (10 8 cells/mL); or (4) a mixture of ChCA or ChFA solutions with bacterial suspensions of the corresponding strains (compositions for treatment were 0.30 mg/mL ChCA+10 8 cells/mL and 0.25 mg/mL ChFA+10 8 cells/mL of bacterial strains).…”

Section: Methodsmentioning

confidence: 99%

“…Potato leaves (250 mg) were immersed in 2.5 mL of distilled water and processed according to [ 35 ]. The optical density of the reaction products was measured on EnSpire equipment (Perkin Elmer, USA) at 522 nm.…”

Section: Methodsmentioning

confidence: 99%

“…Total RNA was isolated from plants with Lira ® reagent (Biolabmix, Novosibirsk, 630090 Russia) according to the manufacturer’s protocol on the 3rd day post infection. Synthesizing of first strand cDNA and the real-time procedure were performed as described previously [ 35 ]. The expression of genes was shown as a fold change normalized to the transcription of the reference gene StAct encoding potato actin.…”

Section: Methodsmentioning

confidence: 99%

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Stimulation of the Defense Mechanisms of Potatoes to a Late Blight Causative Agent When Treated with Bacillus subtilis Bacteria and Chitosan Composites with Hydroxycinnamic Acids

Yarullina,

Cherepanova,

Burkhanova

et al. 2023

Microorganisms

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Phytophthora infestans is, worldwide, one of the main causal agents of epiphytotics in potato plantings. Prevention strategies demand integrated pest management, including modeling of beneficial microbiomes of agroecosystems combining microorganisms and natural products. Chitooligosaccharides and their derivatives have great potential to be used by agrotechnology due to their ability to elicit plant immune reactions. The effect of combining Bacillus subtilis 26D and 11VM and conjugates of chitin with hydroxycinnamates on late blight pathogenesis was evaluated. Mechanisms for increasing the resistance of potato plants to Phytophthora infestans were associated with the activation of the antioxidant system of plants and an increase in the level of gene transcripts that encode PR proteins: basic protective protein (PR-1), thaumatin-like protein (PR-5), protease inhibitor (PR-6), and peroxidase (PR-9). The revealed activation of the expression of marker genes of systemic acquired resistance and induced systemic resistance under the influence of the combined treatment of plants with B. subtilis and conjugates of chitin with hydroxycinnamates indicates that, in this case, the development of protective reactions in potato plants to late blight proceeds synergistically, where B. subtilis primes protective genes, and chitosan composites act as a trigger for their expression.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Stimulation of the Defense Mechanisms of Potatoes to a Late Blight Causative Agent When Treated with Bacillus subtilis Bacteria and Chitosan Composites with Hydroxycinnamic Acids

Yarullina,

Cherepanova,

Burkhanova

et al. 2023

Microorganisms

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“…The leaf content of hydrogen peroxide was measured by the method of Yarullina et al [ 30 ], with modifications. Leaves were homogenized at 4 °C in a mortar in 25 mM of phosphate buffer (pH 6.2; leaf-to-buffer ratio ( m / v ), 1:3) and were centrifuged at 10,000× g and 4 °C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions

et al. 2023

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Adaptation ex vitro is strongly stressful for microplants. Plant-growth-promoting rhizobacteria (PGPR) help to increase the adaptation potential of microplants transplanted from test tubes into the natural environment. We investigated the mechanisms of antioxidant protection of PGPR-inoculated potato microclones adapting to ex vitro growth in an aeroponic system. Potato (Solanum tuberosum L. cv. Nevsky) microplants were inoculated in vitro with the bacteria Azospirillum baldaniorum Sp245 and Ochrobactrum cytisi IPA7.2. On days 1 and 7 of plant growth ex vitro, catalase and peroxidase activities in the leaves of inoculated plants were 1.5-fold higher than they were in non-inoculated plants. The activity of ascorbate peroxidase was reduced in both in vitro and ex vitro treatments, and this reduction was accompanied by a decrease in the leaf content of hydrogen peroxide and malondialdehyde. As a result, inoculation contributed to the regulation of the plant pro/antioxidant system, lowering the oxidative stress and leading to better plant survival ex vitro. This was evidenced by the higher values of measured morphological and physiological variables of the inoculated plants, as compared with the values in the control treatment. Thus, we have shown some PGPR-mediated mechanisms of potato plant protection from adverse environmental factors under aeroponic conditions.

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“…B. subtilis is generally recognized as a safe microorganism for use in the food industry and has been shown to improve and maintain seed quality for extended periods. The growth-promoting and protective effects of the endophytic bacteria B. subtilis have been well-documented in various plant species under different biotic and abiotic stresses [19][20][21][22][23][24]. Because they colonize and live within plant tissues, endophytes are less reliant on external environmental factors compared with rhizospheric microbial strains while also providing beneficial attributes to host plants [22,[25][26][27].…”

Section: Introductionmentioning

confidence: 99%

The Potential of Hydroponic Seed Minituber Enrichment with the Endophyte Bacillus subtilis for Improving the Yield Components and Quality of Potato (Solanum tuberosum L.)

Pusenkova,

Lastochkina,

Ercişli

2023

Agriculture

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This study examined the potential of using the endophytic bacteria Bacillus subtilis (10-4 and 26D) to enrich hydroponically grown potato seed minitubers (Solanum tuberosum L. cv. Bashkirsky) to improve plant growth, photosynthetic pigments, yield, and quality parameters, including nutritional value (i.e., macro-/microelements, vitamin C, anthocyanins). Potato seed minitubers, obtained from in-vitro-grown microplants in a hydroponic system, were inoculated with endophytic B. subtilis and subsequently grown in pots under controlled conditions. The results demonstrated the successful colonization of seed minitubers by B. subtilis, with subsequent distribution into growing plants (roots, shoots). The endophytes accelerated the plant’s phenological shifts, resulting in earlier emergence of sprouts, budding, and flowering compared with control plants. They also had increased leaf photosynthetic pigments (chlorophyll (Chl) a, Chl b, and carotenoids), total leaf area, and positively influenced leaf proline contents. The height of plants and number of stems per plant did not change significantly upon endophyte treatment, but improved root growth was observed throughout the experiment. As a result of endophyte application, there was an increase in stolon weight, number and size of tubers, and overall tuber yield. There were no significant differences in terms of total dry matter and starch content of the tubers compared to the control group, but the sugar levels decreased and the size of the starch grains was larger in endophyte-treated tubers. Furthermore, endophyte treatment resulted in an increased accumulation of nutrients including N, P, K, Cu, and Fe, as well as vitamin C and anthocyanins in harvested tubers. These findings indicate that colonization of hydroponically grown potato seed minitubers with endophytic B. subtilis (10-4 and 26D) before planting has great potential as an eco-friendly approach to obtain higher-quality seeds and to increase tuber yield and nutritional value in field conditions.

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Effect of Bacillus subtilis and Signaling Molecules on the State of the Pro/Antioxidant System and the Expression of Protective Protein Genes in Potato Plants upon Phytophthorosis and a Moisture Deficit

Cited by 3 publications

References 31 publications

Stimulation of the Defense Mechanisms of Potatoes to a Late Blight Causative Agent When Treated with Bacillus subtilis Bacteria and Chitosan Composites with Hydroxycinnamic Acids

Stimulation of the Defense Mechanisms of Potatoes to a Late Blight Causative Agent When Treated with Bacillus subtilis Bacteria and Chitosan Composites with Hydroxycinnamic Acids

Rhizobacteria Increase the Adaptation Potential of Potato Microclones under Aeroponic Conditions

The Potential of Hydroponic Seed Minituber Enrichment with the Endophyte Bacillus subtilis for Improving the Yield Components and Quality of Potato (Solanum tuberosum L.)

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