Effect of Azithromycin on Sciatic Nerve Injury in the Wistar Rats

Ferdowsi, Sevin; Abdolmaleki, Arash; Asadi, Asadollah; Zahri, Saber

doi:10.1007/s11064-022-03721-x

Cited by 3 publications

(4 citation statements)

References 45 publications

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“…According to our ndings, azithromycin can improve sciatic nerve motor and sensory function, reduce oxidative stress and apoptosis in nerve tissue cells, and expedite remyelination. These ndings were congruent with the ndings of Ferdowsi et al, who found that a 150 mg/kg azithromycin injection improved sciatic nerve mobility and sensation, expedited remyelination, and raised neurotrophic factors (Ferdowsi et al 2023). Crocin, like azithromycin, had a signi cant difference with the lesion group in motor, sensory, oxidative stress, apoptosis, and myelin sheath histopathologic tests in our study and caused the sciatic nerve lesion to improve in the measured parameters.…”

Section: Discussionsupporting

confidence: 87%

“…Moreover, recent studies provide evidence in favor of the use of azithromycin as a therapeutic intervention for neurological disorders in neonates, including but not limited to stroke, retinal ischemia, muscular-spinal atrophy, and hypoxic-ischemic brain damage (Amantea et al 2016a;Osman et al 2017). A further investigation revealed that the 7 days administration of azithromycin can accelerate regeneration of sciatic nerve, improve motor and sensory function recovery and upregulate the expression of NGF and BDNF genes after sciatic nerve crush injury in rats (Ferdowsi et al 2023).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Crocin and Azithromycin and their co-administration on experimental model of sciatic nerve injury in rats

Abbaszadeh,

Pourheydar,

Farjah

2024

Preprint

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Background: Although peripheral nerve injury is not life-threatening, it causes significant disability. Following these damages, ischemia and inflammatory processes occur, resulting in neurological dysfunction. Several medications have been explored in order to alleviate the symptoms of peripheral nerve injury. This study aimed to investigate how crocin and azithromycin affected sciatic nerve crush injuries in rats. Methods: Five groups were established using 35 adult male rats: Control, Lesion, Azithromycin (AZ), Crocin (CR), and AZ+CR. Except for the control group, sciatic nerve injury was surgically induced in every other group. For seven days, azithromycin and crocin were administered. Following the completion of biochemical and behavioral evaluations, sections of sciatic nerve were stained in preparation for immunohistochemical and histological analysis. Results: The healing process of sciatic nerve injury was expedited in all experiments by both azithromycin and crocin. However, the efficacy of crocin treatment was more pronounced in comparison to that of azithromycin. Crocin was found to be less efficacious than combination therapy involving AZ and CR, as determined by SFI, hot plate, and immunohistochemical analyses. In the remaining evaluations, however, no significant difference existed between the AZ+CR and CR groups. Conclusion: The recovery process of sciatic nerve injury in rats was enhanced by both crocin and azithromycin; however, the effect of crocin treatment was stronger in the animals. In comparison to the crocin group, combination therapy with azithromycin and crocin did not produce a significant difference in the majority of aspects.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Effects of Crocin and Azithromycin and their co-administration on experimental model of sciatic nerve injury in rats

Abbaszadeh,

Pourheydar,

Farjah

2024

Preprint

View full text Add to dashboard Cite

show abstract

“…In the last decade, the regeneration potential of a number of molecular and gene agents has been evaluated in the mouse sciatic nerve injury model. They have shown potential applications in preclinical studies ( Al-Saedi et al, 2023 ; Elhessy et al, 2023 ; Ferdowsi et al, 2023 ).…”

Section: Preclinical Studies Modeling Peripheral Neuropathies or Peri...mentioning

confidence: 99%

“…The authors showed that the experimental group upregulated NGF and BDNF expression during the treatment, which contributed to improving axon regeneration. They suggested that azithromycin modulated the inflammatory response to improve sensory and motor neuron function ( Ferdowsi et al, 2023 ).…”

Section: Preclinical Studies Modeling Peripheral Neuropathies or Peri...mentioning

confidence: 99%

Neuron-Schwann cell interactions in peripheral nervous system homeostasis, disease, and preclinical treatment

Oliveira,

Yanick,

Wein

et al. 2023

Front. Cell. Neurosci.

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Schwann cells (SCs) have a critical role in the peripheral nervous system. These cells are able to support axons during homeostasis and after injury. However, mutations in genes associated with the SCs repair program or myelination result in dysfunctional SCs. Several neuropathies such as Charcot–Marie–Tooth (CMT) disease, diabetic neuropathy and Guillain–Barré syndrome show abnormal SC functions and an impaired regeneration process. Thus, understanding SCs-axon interaction and the nerve environment in the context of homeostasis as well as post-injury and disease onset is necessary. Several neurotrophic factors, cytokines, and regulators of signaling pathways associated with proliferation, survival and regeneration are involved in this process. Preclinical studies have focused on the discovery of therapeutic targets for peripheral neuropathies and injuries. To study the effect of new therapeutic targets, modeling neuropathies and peripheral nerve injuries (PNIs) in vitro and in vivo are useful tools. Furthermore, several in vitro protocols have been designed using SCs and neuron cell lines to evaluate these targets in the regeneration process. SCs lines have been used to generate effective myelinating SCs without success. Alternative options have been investigated using direct conversion from somatic cells to SCs or SCs derived from pluripotent stem cells to generate functional SCs. This review will go over the advantages of these systems and the problems associated with them. In addition, there have been challenges in establishing adequate and reproducible protocols in vitro to recapitulate repair SC-neuron interactions observed in vivo. So, we also discuss the mechanisms of repair SCs-axon interactions in the context of peripheral neuropathies and nerve injury (PNI) in vitro and in vivo. Finally, we summarize current preclinical studies evaluating transgenes, drug, and novel compounds with translational potential into clinical studies.

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Integrative Analysis Reveals the Expression Pattern of SOX9 in Satellite Glial Cells after Sciatic Nerve Injury

Liu

Yang

et al. 2023

Brain Sciences

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Background: Several complex cellular and gene regulatory processes are involved in peripheral nerve repair. This study uses bioinformatics to analyze the differentially expressed genes (DEGs) in the satellite glial cells of mice following sciatic nerve injury. Methods: R software screens differentially expressed genes, and the WebGestalt functional enrichment analysis tool conducts Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomics (KEGG) pathway analysis. The Search Tool for the Retrieval of Interacting Genes/Proteins constructs protein interaction networks, and the cytoHubba plug-in in the Cytoscape software predicts core genes. Subsequently, the sciatic nerve injury model of mice was established and the dorsal root ganglion satellite glial cells were isolated and cultured. Satellite glial cells-related markers were verified by immunofluorescence staining. Real-time polymerase chain reaction assay and Western blotting assay were used to detect the mRNA and protein expression of Sox9 in satellite glial cells. Results: A total of 991 DEGs were screened, of which 383 were upregulated, and 508 were downregulated. The GO analysis revealed the processes of biosynthesis, negative regulation of cell development, PDZ domain binding, and other biological processes were enriched in DEGs. According to the KEGG pathway analysis, DEGs are primarily involved in steroid biosynthesis, hedgehog signaling pathway, terpenoid backbone biosynthesis, American lateral skeleton, and melanoma pathways. According to various cytoHubba algorithms, the common core genes in the protein–protein interaction network are Atf3, Mmp2, and Sox9. Among these, Sox9 was reported to be involved in the central nervous system and the generation and development of astrocytes and could mediate the transformation between neurogenic and glial cells. The experimental results showed that satellite glial cell marker GS were co-labeled with Sox9; stem cell characteristic markers Nestin and p75NTR were labeled satellite glial cells. The mRNA and protein expression of Sox9 in satellite glial cells were increased after sciatic nerve injury. Conclusions: In this study, bioinformatics was used to analyze the DEGs of satellite glial cells after sciatic nerve injury, and transcription factors related to satellite glial cells were screened, among which Sox9 may be associated with the fate of satellite glial cells.

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Effect of Azithromycin on Sciatic Nerve Injury in the Wistar Rats

Cited by 3 publications

References 45 publications

Effects of Crocin and Azithromycin and their co-administration on experimental model of sciatic nerve injury in rats

Effects of Crocin and Azithromycin and their co-administration on experimental model of sciatic nerve injury in rats

Neuron-Schwann cell interactions in peripheral nervous system homeostasis, disease, and preclinical treatment

Integrative Analysis Reveals the Expression Pattern of SOX9 in Satellite Glial Cells after Sciatic Nerve Injury

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