Effect of azadirachtin on the development of Trypanosoma cruzi in different species of triatomine insect vectors: Long-term and comparative studies

Gonzalez, Marcelo S.; Garcia, Elói S.

doi:10.1016/0022-2011(92)90098-o

Cited by 34 publications

(21 citation statements)

References 16 publications

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“…in R. prolixus infected with T. cruzi Dm28 no parasites were detectable 10 days after azadirachtin treatment, whereas the infection with the VLE strain was eliminated 40 days after the treatment. Using T. infestans the same dierence was evident between the VLE and the Y strain (Gonzalez and Garcia 1992).…”

Section: Discussionsupporting

confidence: 58%

“…Azadirachtin given via a blood meal (before, during, or after infection with T. cruzi) reduces the number of trypanosomes in the gut of R. prolixus and other triatomine species (Garcia et al 1989a;Rembold and Garcia 1989;Garcia and Azambuja 1991;Gonzalez and Garcia 1992). In these investigations, only recently T. cruzi infected bugs were used, i.e., bugs were treated with azadirachtin just 20 days after or before the infection, and laboratory strains of T. cruzi (Dm28, Y or VLE), which had been passaged for a long time in vitro.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of azadirachtin on fresh isolates of Trypanosoma cruzi in different species of triatomines

Kollien

Gonçalves

Garcia

et al. 1998

Parasitology Research

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The effect of azadirachtin was investigated using three different fresh isolates of Trypanosoma cruzi and five different triatomine species which were infected as third-instar larvae. The two T. cruzi strains which originated from sylvatic Triatoma vitticeps showed a high prevalence after the molt to the fifth instar in Panstrongylus megistus and Rhodnius neglectus and a low prevalence in Triatoma infestans and Rhodnius robustus. The third T. cruzi strain originating from a patient in Piaui showed a high prevalence in P. megistus, R. neglectus and T. infestans and a low prevalence in Triatoma sordida. Feeding the infected fifth instars with azadirachtin-supplemented blood (1 microg/ml) resulted 20 days later in some parasite/vector combinations in an increase, in others a decrease or an unchanged number of T. cruzi in comparison to bugs fed with unsupplemented blood.

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Section: Discussionsupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

The effect of azadirachtin on fresh isolates of Trypanosoma cruzi in different species of triatomines

Kollien

Gonçalves

Garcia

et al. 1998

Parasitology Research

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“…The general pattern of infection by the T. cruzi Dm28c clone in the whole gut of Rhodnius prolixus investigated previously (Garcia et al 1989a, b;Gonzalez and Garcia 1992;Gonzalez et al 1999) was similar to the population development in the present investigation. Here, we demonstrate that, in the stomach, the parasite population decreased in all groups.…”

Section: Discussionsupporting

confidence: 80%

Dynamic development of Trypanosoma cruzi in Rhodnius prolixus : role of decapitation and ecdysone therapy

Cortez

Gonzalez

Cabral

et al. 2002

Parasitology Research

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Decapitation and ecdysone therapy on the population dynamics of the Trypanosoma cruzi Dm28c clone in the stomach, small intestine and rectum of fifth-instar larvae of Rhodnius prolixus were investigated. Parasites were not found in the small intestine and rectum of decapitated insects after 10 days post-infection (p.i.). Decapitated ecdysone-supplemented insects sustained the flagellate infection in both gut compartments. In the rectum, the population density of parasites increased 5-fold in ecdysone-treated decapitated larvae and 7-fold in control insects. Epimastigote forms dominated with 40-65%, intermediate stages and round forms varied over 10-35% in the stomach, small intestine and rectum in both insect groups. Low numbers of metacyclic trypomastigotes were observed in the stomach and small intestine of the control group and decapitated insects supplemented with ecdysone but, at 15 days p.i., this form of flagellate reached about 20% in the rectum of the control insects. In the entire gut, at 30 days p.i., 23% of parasites in the control group and 8% in the decapitated insects treated with ecdysone were found. These results indicate that a head factor, possibly the prothoracicotropic hormone from the brain which stimulates ecdysone production by the prothoracic glands, may act directly or indirectly to stimulate the development of epimastigotes and round forms of the parasite and that a single ecdysone treatment is not able to fully reverse metacyclogenesis in decapitated R. prolixus.

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“…Garcia's group demonstrated that azadirachtin applied at different intervals before, during, or after T. cruzi infection, reduces the number of parasites in the gut (Garcia et al 1989a,b). A simple dose of azadirachtin is able of preventing infection or re-infection of the vector by the parasites for long time (Gonzalez & Garcia 1992). After feeding R. prolixus with blood infected with T. cruzi clone Dm28c without azadirachtin, the number of parasites increases 15 fold within three weeks of infection, but in the azadirachtin fed insects the infection drops within the same period to 14%, and after 30 days no parasites can be detected (Garcia et al 1989a,b).…”

Section: Trypanosoma Cruzi and Triatomine Neu-roendocrine Interactionmentioning

confidence: 99%

“…After feeding R. prolixus with blood infected with T. cruzi clone Dm28c without azadirachtin, the number of parasites increases 15 fold within three weeks of infection, but in the azadirachtin fed insects the infection drops within the same period to 14%, and after 30 days no parasites can be detected (Garcia et al 1989a,b). In addition, to the total loss of the ingested parasites, a later infection with T. cruzi is inhibited for up to 120 days at least (Gonzalez & Garcia 1992). Finally, a direct toxic effect on the parasite can be nearly excluded, becasue T. cruzi cultivated in LIT-medium are not affected by annaddition of azadirachtin.…”

Section: Trypanosoma Cruzi and Triatomine Neu-roendocrine Interactionmentioning

confidence: 99%

Biological factors involving Trypanosoma cruzi life cycle in the invertebrate vector, Rhodnius prolixus

Garcia

Gonzalez

1999

Mem. Inst. Oswaldo Cruz

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Effect of azadirachtin on the development of Trypanosoma cruzi in different species of triatomine insect vectors: Long-term and comparative studies

Cited by 34 publications

References 16 publications

The effect of azadirachtin on fresh isolates of Trypanosoma cruzi in different species of triatomines

The effect of azadirachtin on fresh isolates of Trypanosoma cruzi in different species of triatomines

Dynamic development of Trypanosoma cruzi in Rhodnius prolixus : role of decapitation and ecdysone therapy

Biological factors involving Trypanosoma cruzi life cycle in the invertebrate vector, Rhodnius prolixus

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