Effect of Avocado (Persea Americana Mill.) Peel Extract on the Diabetic Male White Rats: Preclinical Study

Abstract

Background: Type 2 Diabetes Mellitus (DMT2) is one of the most common non-communicable diseases (NCDs) worldwide. T2DM is also the leading cause of death in most developed and developing countries.
 Objective: This study aimed to analyze the effect of avocado (Persea americana Mill.) skin extract on blood glucose levels in white male rats with diabetes (preclinical test).
 Method: Experimental research in vivo with True Experimental-PreTest and Post Test Only Control Group Design. The samples were 15 white male rats aged ±3 months, with a 150-200 grams bodyweight. Avocado peel was extracted with ethanol. Measurement of fasting blood glucose levels was carried out 3 times. Streptozotocin administration was used to increase glucose levels in experimental animals. Data analysis used Analysis of Variance (ANOVA) to analyze the data obtained from each treatment group, and the level of significance was expressed in =5%.
 Results: Fasting blood glucose levels in the first measurement for all groups were included in the normal category, about 48.73 mg/dL. Then the second measurement after being induced with STZ showed an increase in fasting blood glucose levels of mice with an average of 181.07 mg/dL. Furthermore, the 3rd measurement showed that mice's fasting blood glucose level was still high, except for group IV, which decreased to 97.33 mg/dL. The results of the comparative analysis of fasting blood glucose levels on the 2nd and 3rd measurements showed that most of them experienced a decrease, except in group V, and the intervention group which experienced a significant decrease, group IV with p-value = 0.003 and the magnitude of the decrease was 133.33 mg/dL.
 Conclusion: The concentration of 200 mg/kg BW of avocado peel extract significantly reduced fasting blood glucose levels by 133.33 mg/dL after STZ induction compared to other groups

The Effect of Giving Avocado Leaf and Seed Extract as Antidiabetes: A Systematic Literature Review

“…The results showed that the dose of avocado leaf extract affected the level of kidney damage, where the higher the dose of the extract given, the lower the level of kidney damage that occurred. The 200 mg/kg bw dose of avocado leaf extract showed the most positive results with no detectable signs of kidney damage (Rahman et al, 2022).…”

“…The research results of Rahman et al (2022) regarding the effect of avocado peel extract on male white rats with diabetes showed differences in response to fasting blood glucose levels between the treatment groups (Rahman et al, 2022). The results showed that avocado peel extract at certain doses did not significantly reduce fasting blood glucose levels in several treatment groups.…”

“…Follow-up research conducted by Rahman et al (2020) showed that avocado leaf extract has the potential to increase kidney cell regeneration in male white rats with diabetes (Rahman et al, 2022). The results showed that the dose of avocado leaf extract affected the level of kidney damage, where the higher the dose of the extract given, the lower the level of kidney damage that occurred.…”

“…The effect of avocado peel extract on fasting blood glucose levels can vary depending on the dose given and the characteristics of each group. The group that showed a decrease in fasting blood glucose levels shows the potential of avocado peel extract as an antidiabetic agent to reduce blood glucose levels (Rahman et al, 2022). However, further research is needed in the group experiencing elevated fasting blood glucose levels to understand the mechanisms and factors contributing to these different effects.…”

Skrinning Diabetes Melitus Dan Pemberian Jus Alpukat Pada Ibu Hamil Untuk Mencegah Diabetes Melitus Gestasional

“…Hasil penelitian dari (Rahman et al, 2022)juga menemukan bahwa konsentrasi 200 mg/kg BB buah alpukat ekstrak kulit secara signifikan mengurangi glukosa darah puasa kadarnya sebesar 133,33 mg/dL setelah induksi STZ dibandingkan kepada kelompok lain. Konsentrasi yang terlalu rendah dan tinggi ekstrak kulit alpukat tidak menunjukkan pengaruh yang signifikan dan perubahan positif dalam meningkatkan glukosa darah puasa tingkat pada tikus (Rahman et al, 2022).…”