Effect of autologous platelet-rich plasma on the chondrogenic differentiation of rabbit adipose-derived stem cells in vitro

Abstract

Abstract. This study aimed to isolate rabbit adipose-derived stem cells (ADSCs) and explore the potential of platelet-rich plasma (PRP) in the chondrogenic differentiation of ADSCs, thereby potentially providing a new approach for the repair and regeneration of cartilage injury. Rabbit ADSCs were isolated and characterized by induction towards adipogenic, osteogenic and chondrogenic lineages in vitro. The isolated ADSCs were also cultured with or without 10% PRP. Immunofluorescence staining, toluidine blue staining and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to detect type II collagen (Col II) and aggrecan (AGC) expression. Col II immunofluorescence staining and toluidine blue staining indicated that following induction by autologous PRP, ADSCs manifested Col II and AGC expression. The expression of Col II and AGC mRNA was significantly upregulated in the PRP-treated cells when compared with that in control cells. Autologous PRP produced by laboratory centrifugation was able to promote the chondrogenic differentiation of rabbit ADSCs in vitro.

Generation of Insulin‐Producing Cells From Human‐Induced Pluripotent Stem Cells Using a Stepwise Differentiation Protocol Optimized With Platelet‐Rich Plasma

“…Therefore, platelets in the differentiation procedure slowly and steadily burst and release the adhesion molecules and growth factors into the culture medium, and this improved the differentiation process and lack of unwanted differentiation to other tissues. These results aligned with the study by Tang et al (2015). Stimuli in the process of differentiation led to a change of the cell cycle in iPSCs significantly.…”

“…As a result, the methods should be safer and less expensive. These results aligned with the study by Tang et al (2015). When the PRP is activated, it releases a lot of growth factors and adhesive molecules implicated in the differentiation of stem cells to IPCs such as bFGF, EGF, HGF, VEGF-A, and fibronectin and Ngn3, Insulin, Glucagon, and Glut2 were analyzed at each group of differentiation into IPCs.…”

“…Platelet-rich plasma was prepared using two centrifugation steps (Lahmy et al, 2014;Tang et al, 2015;Burnouf et al, 2016;Masoudi et al, 2016). Briefly, 10 ml human fresh blood was collected into tubes containing 1 ml acid-citrate-dextrose solution as an anticoagulant.…”

Phase II Multicenter Randomized Controlled Clinical Trial on the Efficacy of Intra-articular Injection of Autologous Bone Marrow Mesenchymal Stem Cells with Platelet Rich Plasma for the Treatment of Knee Osteoarthritis

“…However, due to the different methods of production standardization is di cult and there is an open debate concerning the interpretation of the research on PRP [21,22]. In any case, the identi cation of PRP-derived anabolic growth factors (basic FGF, TGF-β1, TGF-β2, EGF, IGF-I, PDGF-AB, PDGF-BB, VEGF) and anti-in ammatory cytokines (IL-1ra, sTNF-R1, sTNF-RII, IL-4, IL-10, IL-13, IFNγ) may present therapeutic potential in the treatment of osteoarthritis, especially in combination with MSCs, through enhancing or preserving their regenerative properties [23][24][25]. In addition, a positive effect on joint lubrication through stimulation of synoviocytes-derived hyaluronic acid has been reported for PRP, thus, when administered as co-adjuvant of MSCs, PRP could increase the intra-articular retention and survival of MSCs in an anti-in ammatory environment [26,27].…”

Phase II Multicenter Randomized Controlled Clinical Trial on the Efficacy of Intra-articular Injection of Autologous Bone Marrow Mesenchymal Stem Cells with Platelet Rich Plasma for the Treatment of Knee Osteoarthritis

“…there is an open debate concerning the interpretation of the research on PRP [21,22]. In any case, the identi cation of PRP-derived anabolic growth factors (basic FGF, TGF-β1, TGF-β2, EGF, IGF-I, PDGF-AB, PDGF-BB, VEGF) and anti-in ammatory cytokines (IL-1ra, sTNF-R1, sTNF-RII, IL-4, IL-10, IL-13, IFNγ) may present therapeutic potential in the treatment of osteoarthritis, especially in combination with MSCs, through enhancing or preserving their regenerative properties [23][24][25]. In addition, a positive effect on joint lubrication through stimulation of synoviocytes-derived hyaluronic acid has been reported for PRP, thus, when administered as co-adjuvant of MSCs, PRP could increase the intra-articular retention and survival of MSCs in an anti-in ammatory environment [26,27].…”